Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.8% 6171.9 / 0.7 2103intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (7/40);  new base T (1290/766);  total (1297/806)
Fisher's exact test for biased strand distribution p-value = 3.20e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2167 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347
                                                                                                                             |                                                                                                                                 
tttACTTTAATTGAATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  1:3609027/128‑1 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                             <  2:4161988/128‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            <  2:3791183/128‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  1:3006386/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  1:3864977/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  2:4055158/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  1:1724391/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                        <  2:1018780/128‑1 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTATAAAAaattaat                                                                                                                        >  1:2169328/1‑128 (MQ=255)
          ttGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaatt                                                                                                                       >  1:5188143/1‑128 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:3682930/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:5500674/128‑1 (MQ=255)
           tGTAATACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:2580167/128‑1 (MQ=255)
            gtttttatttactAGTTGATAATATTTTTTTTAATTAATTTTTAGCACATGTTTTAATTTGTAATAAAAAAAAAATAATATCTTATTTTATATTATTTTTTTATTCAAATTTTTATAAAATTAAtttt                                                                                                                     >  2:1455922/8‑127 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGa                                                                                                                    >  1:738849/1‑128 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAtttaa                                                                                                                    >  1:2649057/1‑126 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                    <  2:5022266/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   >  1:4261198/1‑128 (MQ=255)
                  ctttactAGTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                               <  1:503751/128‑1 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                             >  2:3351728/1‑128 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTATaaa                                                                                                           >  2:1915052/1‑128 (MQ=255)
                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                          <  2:3004002/128‑1 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAAATAATTTGAACTTTTAAAAAATa                                                                                                      >  1:3985028/1‑128 (MQ=255)
                             gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGc                                                                                                    >  2:594992/1‑128 (MQ=255)
                               taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                  >  2:27399/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTATTTGTATATGCTAATTTTTAATAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                 >  2:2186300/1‑128 (MQ=255)
                                 atatTTATTTTAATTAATTTATCGCACTTGTATGTATTATTAATAAACTAAAACTATTATCTTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCtttt                                                                                                <  1:486607/128‑1 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  1:5265152/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  1:4437422/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  2:323927/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  2:3912684/1‑128 (MQ=255)
                                  tatttataatCATTAATTTAACGCAATTGGATGAATTAGTAAGAAACTAAAACAATAATCTTTTTAGAAATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACATGTAAAAAATAGCttttt                                                                                               <  1:2482971/119‑1 (MQ=255)
                                    tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                             >  1:154406/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                         >  2:4392734/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                         >  1:3192681/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtattttttttttt                                                                                       >  2:4487181/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                    >  1:4525663/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                    >  1:5094172/1‑128 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTATTTTTAACTTGTAAAAAATATAttttttttttttttttt                                                                                   >  2:1121618/1‑128 (MQ=255)
                                              ttAATTTATAGTAATTGTATTAATTATTAATAAAATAAAACTATTATATTTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAAATTGAAATTTTAAAAAATAGCttttttttttttttata                                                                                   <  1:1608976/128‑4 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttattttttttttttaataat                                                                               >  1:1335185/1‑122 (MQ=255)
                                                   ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttatat                                                                              >  2:4940222/1‑124 (MQ=255)
                                                    tATCGCAATTGTATTAATTAGTAATAAAATAAAACTATTATTTTTTTATATATTTTTTTTATATGAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCttttttttttttttttataatgg                                                                             <  2:4263413/128‑8 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAATTTTTTAAAAAATTAATTTGAACTTGTATAAAATATCtatttttttttttatttaaatttata                                                                          >  1:2726619/1‑119 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatttaaatat                                                                      >  1:665889/1‑118 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatttaaaaat                                                                      >  1:849746/1‑118 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAatat                                                                    >  1:4783386/1‑128 (MQ=255)
                                                             tGTATTAATTAGTAATAAAATAAAAATATTATATTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                  <  1:1010645/128‑1 (MQ=255)
                                                             tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatgtaattaaaa                                                                    >  1:3090473/1‑116 (MQ=255)
                                                              ttttttatttataataaAATAAAAATAATTTTTTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTTATTTGTACTTTTAAAAAATTGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                 <  2:313061/117‑1 (MQ=255)
                                                               tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATTTAAATaaaa                                                                  >  1:1985534/1‑128 (MQ=255)
                                                               tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGTAAATATaaa                                                                 >  1:22814/1‑128 (MQ=255)
                                                                tttatatAGTAAAAAACAAAAACTATTAACTTTTTATATTTTTTTTGTATAAGCAAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  1:2837870/122‑1 (MQ=255)
                                                                    aTTAGTAAGAAACTAAAACCATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAAtttt                                                             >  1:1813809/1‑128 (MQ=255)
                                                                      tactaataaAATAAAACATTTTTATTTTTTTTTTTTTTTTTTATATTTAAATTTTTAAAAAATTAATTTGAACTTTTTAAAAAATTCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                           <  1:2926161/125‑1 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATTTATATTTTAgtt                                                         >  2:1984883/1‑126 (MQ=255)
                                                                       atttatataataaaaataATATCTTTTTTTTTATTTTTTTTATTTTTAAATTTTTTAAATATTAATTTTAATTTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                         <  1:2091019/118‑1 (MQ=255)
                                                                       attatctaattaaaacttTTTTATTTTTTTTTTTTTTTTTTTTATGCAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAAT‑‑TTGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                        <  2:5095935/118‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATAATAATTTTTGGt                                                         >  2:1569237/1‑128 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATtatatttat                                                       >  1:2557270/1‑123 (MQ=255)
                                                                        ttaatataaaaaaaataTTATATTTTTATTTTTTTTTTTTTTTATTAAATTTTTTAAAAATTAATTTTTAATTTTAAAAAAAA‑‑TCCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                       <  1:4177269/118‑1 (MQ=255)
                                                                        agaaataacaaaaaataTTATCTTTTTAGATATTTTTTGTTTATTCAAATTTTTAAAAAAATAATTTTTACTTGTAAAAAATAGGGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                        <  1:4683219/122‑1 (MQ=255)
                                                                          aattaaataaaactaTTTTTTTTTTATTTATTATTTGTATTTTTAAATTTTTAAAAATTTATTTTGAACTTTTAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                     <  2:3657156/121‑1 (MQ=255)
                                                                            gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTAttttt                                                   >  1:1430376/1‑127 (MQ=255)
                                                                            gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTTAAAAATAAATTTTATATAttt                                                     >  1:4098172/1‑128 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGGAAATATAAATTTTAGTTATtttttt                                                 >  2:5261477/1‑128 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATTTAAATATAAATTTTAGTTATtttttt                                                 >  2:3542043/1‑128 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATTAAAATTTTatttttttttttt                                                >  2:3436987/1‑116 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAATTAAAAATTTTAGGTATTTTTTTTTc                                              >  2:2408230/1‑128 (MQ=255)
                                                                                 aaaaaataTTATTTTTTTATTTATTTTTTGTATATGAAAAATTTTAAAAAATTAATTCGAACTTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                            <  1:3283400/127‑1 (MQ=255)
                                                                                  aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAAAAAAAATTTTAGGTATTTtgttgta                                              >  1:4610362/1‑127 (MQ=255)
                                                                                      attatatCTTTTTTTTTATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                          <  2:5375580/124‑1 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGTAAATTAAAATTTTTGTTATTTTTTTGTATaatt                                         >  2:4714589/1‑126 (MQ=255)
                                                                                        atttttttttttTATATTTTTTGTTTTTTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                      <  1:5481616/122‑1 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGtatatttat                                       >  1:952312/1‑123 (MQ=255)
                                                                                            tCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAGGGAAATATAATTTTTAGTTTTTTTTTTGTATaattttatta                                   >  2:1818980/1‑120 (MQ=255)
                                                                                              tttttAATTTTTTTTTTATATGTAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAAAA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                               <  1:4359052/128‑1 (MQ=255)
                                                                                               ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATCTTAGTTATTTTGTTTTATAATTTTATaaaaa                                >  2:2485883/1‑128 (MQ=255)
                                                                                                tttttatatTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                              <  1:5351281/123‑1 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTAAAAATTTAAATATAAATTTTAATTATTTTTTTTTAAAAATATATaaaaaaa                              >  1:3513333/1‑128 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTGTTTTATAAGTATATaaaaaaa                              >  1:1251117/1‑128 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAAt                             >  1:3770391/1‑128 (MQ=255)
                                                                                                    tatatTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                          <  2:4587706/127‑1 (MQ=255)
                                                                                                    gttatTTTTTGTATTTGTAAATTTTTAAAAAATTAAATTTAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  2:1682162/126‑1 (MQ=255)
                                                                                                           tttttattttaaaaTTTTTTAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  1:49663/117‑1 (MQ=255)
                                                                                                            ttttttttttaaaTTTTTTAAAAATTTATTTTAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  1:1170461/118‑1 (MQ=255)
                                                                                                            ttttataTTTAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  2:816482/125‑1 (MQ=255)
                                                                                                               ttattaaaatttttATAAAATTTAATTTTAATTTTTAAAAAAA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat               <  2:679269/118‑1 (MQ=255)
                                                                                                               tataTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTATATAATGGAAATATAAATTTTAGGTATTTTTTTGTATAATTTTATAAAAAAATATAAATGAAAAAata              >  1:1911767/1‑128 (MQ=255)
                                                                                                                 tttttaaaTTTTTAAAAAATTAATTTTTACTTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat               <  1:109808/123‑1 (MQ=255)
                                                                                                                  atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTATTTTTTTTGTATATTTATATAAAAAAATAGAAATGAAAAAAtttttt          >  2:2303037/1‑126 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATTTATATTTTAAGAATTTTTTTTTAAAATATTATAAAAAAATAaaaaaaaaaaaaatt             >  2:1274082/1‑125 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATTATGAAAAAATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAAAATAAATGAAAAAATAtt            >  2:1346188/1‑128 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGTAATTTTTTTTTGAATTTATATAAAAAAATATAAAGTaaaaaaatttttt         >  1:2348487/1‑121 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAAAAAAAATTTAATTTATTTTTTTGTATAATTAAAAAAAAAAATTAAAAAAAAAAAAAATTTTTa        >  2:904335/1‑128 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATAGAAAGTAAAAAAAATTTTTaa       >  2:2093559/1‑128 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGGTGTCTAATTATCTAAAAAAATAGAAAGTAAAAAATATTGTaaa       >  2:2881606/1‑128 (MQ=255)
                                                                                                                         ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTAGTTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATTTTTTTaaa      >  1:3411771/1‑128 (MQ=255)
                                                                                                                         ttttAAAAAATTTATTTGAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaaa     <  2:4820999/128‑1 (MQ=255)
                                                                                                                         ttttAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGGAAATATAAATTTTAGGTATTTTGTTGTCTATGTATCTAAAAAAATATAAAGTAAAAAATATTTTTaaa      >  1:558215/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                                 
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165093‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: