Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA99.0% 6386.1 / 37.7 2092intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (1281/777);  minor base . (4/17);  total (1289/803)
Fisher's exact test for biased strand distribution p-value = 1.11e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2142 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350
                                                                                                                               |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTaaaaaa                                                                                                                                >  2:1419152/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAaat                                                                                                                               <  2:3791183/128‑1 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAaat                                                                                                                               <  2:100170/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                         <  2:5500674/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                         <  1:5325911/128‑1 (MQ=255)
       tGTATTAATTTTCTAGTTGATATTATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAATACTATTTTCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                         <  2:4399974/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTg                                                                                                                        <  1:2999830/128‑1 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTTaa                                                                                                                      >  1:2476183/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGaa                                                                                                                      >  1:4513439/1‑128 (MQ=255)
              ctttactAGTTGATAATATTTAGTTTCATTATTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTg                                                                                                                  <  2:687686/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 <  2:3311582/128‑1 (MQ=255)
                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAAATTGTa                                                                                                                >  1:726450/1‑128 (MQ=255)
                 tactAGTTGATACTATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               <  1:2819487/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               >  1:2645605/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             <  2:2169759/128‑1 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                            >  1:3164262/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                           >  1:4950078/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGc                                                                                                       >  2:594992/1‑128 (MQ=255)
                          ataataTTTAGTTTCATTAATTTATAGCACTTGTATGAATTAGTAACAAACTAAAACTATTATCTTTTTATATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCt                                                                                                      <  1:1169780/128‑1 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                    >  2:271285/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttt                                                                                                   >  1:2554399/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                   >  1:3268356/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttt                                                                                            >  1:2167846/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCtatttttttttt                                                                                           >  2:1099648/1‑128 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                       >  1:5028424/1‑128 (MQ=255)
                                          ttAATTTATCTCAATTTTTTGAATTAGTAATAAAATAAAAATATTATTTTTTTATATATTTTTTT‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCtttttttttttttttat                                                                                      <  1:4669597/128‑3 (MQ=255)
                                          ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATAtttttttttttttttaa                                                                                      >  1:3445729/1‑126 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatat                                                                                  >  2:2561365/1‑128 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaaatttaaaaa                                                                         >  2:2375188/1‑118 (MQ=255)
                                                        ttGTATGTATTATTAAAAAAAATAAAATATTTTATTTTTTTATATTTTTTT‑TTTTTTAAAA‑TTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                      <  2:3432683/128‑1 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAAaata                                                                      >  2:3224251/1‑128 (MQ=255)
                                                         tGTATTAATTAGTAATAAAATAAAAATATTATATTTTTAGATATTTTTTT‑TATATGCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                     <  1:1010645/128‑1 (MQ=255)
                                                          ttttttaTTAGTAATAAAATAAAAATATTTTATTTTTTTAATATTTTTG‑TTAATTCAAT‑TTTTTAAAAAATTTATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                    <  2:5261801/122‑1 (MQ=255)
                                                          gTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                                   <  1:458893/128‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAAttt                                                                 >  1:1619416/1‑128 (MQ=255)
                                                               taTTAGTTATAAAATAAAAATATTATTTTTTTTTTTATTTTTTT‑TTTTTTTAAA‑TTTTTTAAAAATTAATTTGAAATTTTTAAAAATTGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                               <  1:3953127/127‑1 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTat                                                              >  1:5082404/1‑127 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACCATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAAtttt                                                                >  1:1813809/1‑128 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAATAAATAGCTTTTTTTTTTTTTTTTTAT‑TATTTGAATTTTAAAtttttat                                                             >  2:4157449/1‑125 (MQ=255)
                                                                   attaataaACTAAAAAAATATTATTTTTATTTATTTTTTG‑TATATTCAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                           <  2:3117510/126‑1 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTatttattt                                                        >  1:4426835/1‑128 (MQ=255)
                                                                     taataaACTAAAAATATTATTTTTTTAGATATTTTTTT‑TATATGCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                        <  1:827593/128‑1 (MQ=255)
                                                                       atatattaaaactaTTTTATTATTAGATAATTTTTT‑TATTTTTAAA‑ATTTTAAAAAATTTATTTGTTCTTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtttt                                                       <  1:3570153/122‑1 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTAttttt                                                      >  1:1430376/1‑127 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTAGTTAtttttt                                                     >  1:3076375/1‑126 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAGTTAtttttt                                                     >  1:124922/1‑126 (MQ=255)
                                                                         aaaataaaaattTTTTTTTTTTTTATATTTTTTT‑TAAATGCAAA‑ATTTTAAAAAATTAATTTTTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                    <  2:4490582/128‑1 (MQ=255)
                                                                         aaaataaaaataTTATATTTTTTTTTATTTTTTT‑TTTTTTTAAA‑TTTTTAAAAAATTTATTTTAAATTTTTAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                      <  2:1906356/128‑1 (MQ=255)
                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTATATAATATAAATAAAAATTTAAATTATtttttta                                                   >  1:556399/1‑127 (MQ=255)
                                                                            ataaaaataTTTTGTTTTTTTTTATTTTTTT‑TATATTTAAA‑TTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                  <  1:2347288/127‑1 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTA‑AATGTAAATTTAAATTTTTGGTATTTTTTTTTCt                                                >  2:3529841/1‑128 (MQ=255)
                                                                               aaactaTTATATTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                              <  1:2711666/128‑1 (MQ=255)
                                                                                  cttttttCTTTTTTTATATTTTTTG‑TATATGTAAA‑TTTTTAAAAAATTTTTTTTAACTTTGAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGt                                            <  1:1552895/122‑1 (MQ=255)
                                                                                   tattatTTTTTATTATATTTTTTT‑TATTTGTAAA‑TTTTTAAAAAATTAATTTGAACTTTTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                          <  1:3751793/128‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAGTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTTAATATTAATTTTAGTTATTTttttgtaatatttt                                          >  2:1628222/1‑120 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑‑‑ATGGAAATATAAATTTTATTTAATTttttgtaatagtat                                          >  2:5113915/1‑120 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTATAATTa                                           >  1:1980185/1‑128 (MQ=255)
                                                                                    attttttttttATATATTTTTTT‑TTTATTAAAATTTTTAAAAAAATAAATTTAAATTTTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                           <  1:2089694/122‑1 (MQ=255)
                                                                                    attatCTTTTTTGATATATTTTG‑TTTATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                          <  1:4840434/128‑1 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATTTAAATATAAATTTTATTTATttttttttatattttt                                          >  2:4941586/1‑118 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATATAATTTTTAGGTATTTTTTTGACTAAGTAt                                          >  2:3653805/1‑128 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTAta                                         >  1:3366167/1‑127 (MQ=255)
                                                                                    attatAATTTTATAAATTTTTTT‑TATATTAAAT‑TTTTTAAAAATTAAATTTTAATTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                          <  1:1914535/128‑1 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑TTTTTTTTTTTTTTTTTAATATTTTAAATATAAATTTTAGGTATTtttttgaaaaatttt                                          >  2:4270037/1‑119 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAta                                         >  2:5026375/1‑127 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTAGTTATTTTGTTTTATAATTAta                                         >  1:5455703/1‑127 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTAAAAGTAta                                         >  1:5183105/1‑127 (MQ=255)
                                                                                      tttttatttATATATTTTTTGTTATAT‑CAAA‑TTTTTAAAAAATTAATTTTAAATTTTTAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                         <  2:2845043/122‑1 (MQ=255)
                                                                                      tatTTTTTTAGATATTTTTTT‑TATATGAAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                        <  1:4301946/128‑1 (MQ=255)
                                                                                          tttttAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTTAATTAAAAATTTTAGTTATTTTTTTTTATAATTATATaaaaa                                   >  2:2069052/1‑128 (MQ=255)
                                                                                          tttttAGATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATTTATTTTTTTGTATAATTATATaaaaa                                   >  2:2470445/1‑128 (MQ=255)
                                                                                           ttttAGATATTTTTTG‑TATATTCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATAAAAATATAAATATTATTAATTTTTATTTCTAATTATATaaaaa                                   >  1:2909998/1‑128 (MQ=255)
                                                                                            tttAGAAATTTTTTT‑TGAATTGAAA‑TTTTTAAAAAATTAATTTTAATATTTAATAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                                  <  1:3647523/128‑1 (MQ=255)
                                                                                                tttatTTTTTT‑TTTATGAAAA‑TTTTTTAAAAATTTATATTAATTTTTAAAAAAAA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTTTCTAAGTATCTAAAAAATTata                             <  2:537807/126‑3 (MQ=255)
                                                                                                tattttttttt‑tttATTTAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGG‑AATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                             <  2:1218481/124‑1 (MQ=255)
                                                                                                tatatTTTTTG‑TTTATGCAAA‑TTTTTAAAAAATTAATTTTTACTTGTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                             <  2:4587706/127‑1 (MQ=255)
                                                                                                gATATTTTTTG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                          <  1:654953/128‑1 (MQ=255)
                                                                                                    ttttttG‑TTAATTCAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                           <  1:4225598/128‑1 (MQ=255)
                                                                                                        ttG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATATAAAAAATTAGAAATGaaa                      >  2:1520931/1‑128 (MQ=255)
                                                                                                         ttttttttttaaa‑TTTTTTAAAAATTAATTTTTAATTTTATAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  2:2289731/118‑1 (MQ=255)
                                                                                                         ttttataTTTAAA‑TTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  2:816482/125‑1 (MQ=255)
                                                                                                         tG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTATTTTTCTAAAAAAATATAAATGaaa                      >  1:4751913/1‑128 (MQ=255)
                                                                                                         tG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATATTTTTCTAAAAAAATATAAATAaaaa                     >  1:4058867/1‑128 (MQ=255)
                                                                                                         tG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAATAAAAAATTTTATTTATTTTTTTTTATAAATATAAAAAAAAAAATAAATAaaaa                     >  2:1962475/1‑128 (MQ=255)
                                                                                                         tG‑TATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATAAATTaaaaaa                   >  1:1332328/1‑128 (MQ=255)
                                                                                                          tttataTTCAAA‑TTTTTAAAAAAATAATTTGTACTTTTAAAAAAAA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                    <  2:4716922/126‑1 (MQ=255)
                                                                                                             ttatttaaa‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaata                  <  1:1726003/122‑3 (MQ=255)
                                                                                                              ttttttaa‑TTTTTTAAAAATTAATTTTTATTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAATGAAAAAatat                <  2:3913073/122‑1 (MQ=255)
                                                                                                              tttttaaa‑TTTTTAAAAAATTAATTTTTACTTTTTAAAAATA‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                  <  1:109808/123‑1 (MQ=255)
                                                                                                              taagtaaa‑TTTTTAAAAAATTTATTTTAAATTTT‑‑AAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat                <  1:4141669/123‑1 (MQ=255)
                                                                                                                tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTAATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAata                 >  1:2268148/1‑128 (MQ=255)
                                                                                                                tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTGTATAAGTATATAAAAAATTATAAAGTAAAAAatat                >  1:382106/1‑128 (MQ=255)
                                                                                                                  cAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTATTATAATGTAAAAATAAATTATAATAATTTTTTATTCTAAATAACTAAAAAAATAAAAATAAAAAAATAtttt             >  1:925665/1‑126 (MQ=255)
                                                                                                                   aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAAAAAAAATTTAATTTATTTTTTTGTATAATTAAAAAAAAAAATTAAAAAAAAAAAAAATTTTTa           >  2:904335/1‑128 (MQ=255)
                                                                                                                       ttttTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAg       <  2:1332328/128‑1 (MQ=255)
                                                                                                                        tttAAAA‑AATTAATTTGAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  2:2988408/128‑1 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTTTAAATGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt      <  2:1791395/126‑1 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTATAATTATATAAAAAAATATAAATTAAAAAATATTTTTaaaatt      >  1:5259258/3‑126 (MQ=255)
                                                                                                                           taaaaaaTTAATTTGAACATTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  2:4824002/127‑1 (MQ=255)
                                                                                                                           aaaaaaTTTAATTTTAAATTTTAAAAATT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  2:406255/128‑1 (MQ=255)
                                                                                                                               |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTG‑TATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑