Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,163 2 bp→TT intergenic (‑446/+396) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1630AT99.5% 6203.2 / NA 1967intergenic (‑446/+397)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base T (1055/901);  total (1059/908)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1640AT98.5% 5548.0 / NA 1950intergenic (‑447/+396)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/25);  new base T (1049/871);  total (1054/896)
Fisher's exact test for biased strand distribution p-value = 1.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1969 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420041‑420285
                                                                                                                          ||                                                                                                                                 
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                             <  2:1868041/128‑1 (MQ=255)
    catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                           <  2:1122131/128‑1 (MQ=255)
      tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                         <  2:802077/128‑1 (MQ=255)
          tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                     <  1:984261/128‑1 (MQ=255)
           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                    <  1:4482552/128‑1 (MQ=255)
           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                    >  1:3225514/1‑128 (MQ=255)
           ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                    >  1:2391/1‑128 (MQ=255)
             aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                                  >  1:3326580/1‑128 (MQ=255)
                ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                               <  2:688544/128‑1 (MQ=255)
                ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                               <  2:1279027/128‑1 (MQ=255)
                ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                               >  2:2143939/1‑128 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                              <  1:4098240/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                              <  2:4529572/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                              <  1:2235730/128‑1 (MQ=255)
                     aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAATTCAgttattaaaattaaaa                                                                                                          <  2:1503890/128‑11 (MQ=255)
                     aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                          >  1:4646988/1‑128 (MQ=255)
                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                         <  1:4745347/128‑1 (MQ=255)
                       aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                        >  1:610050/1‑128 (MQ=255)
                       aTCTAAGTTTCTAAAATTATATTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                        <  2:3750265/128‑1 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                     <  1:4829349/128‑1 (MQ=255)
                           aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                    <  2:2700477/128‑1 (MQ=255)
                             gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGATAAatta                                                                                                  >  2:3036727/1‑128 (MQ=255)
                               atCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                                <  2:2560547/127‑1 (MQ=255)
                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                               <  2:2455513/128‑1 (MQ=255)
                                 cTAAAATTCTTATATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              <  2:4115404/128‑1 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATAGTTAAATTAtttt                                                                                              >  1:3476927/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  1:2834664/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  1:3627062/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  2:347163/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                              >  1:5094862/1‑128 (MQ=255)
                                  tAAAATTATATTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                             <  1:1000427/128‑1 (MQ=255)
                                   aaaaTTATTTTATGATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                            <  1:4181835/128‑1 (MQ=255)
                                    aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                           >  1:5259794/1‑128 (MQ=255)
                                    aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                           <  1:2021872/128‑1 (MQ=255)
                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                          <  1:1892436/128‑1 (MQ=255)
                                       ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                        >  1:4561419/1‑128 (MQ=255)
                                        tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                       >  1:1593971/1‑128 (MQ=255)
                                        tattTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATATTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                       >  1:1341824/1‑128 (MQ=255)
                                          ttttATAATTTAAAAATATAATACTTTTTTTAGTTGATATATTAGTAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAAATATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                     <  2:5359405/128‑1 (MQ=255)
                                             tATCATTAAAAAAAATATTATTTTTATATGTTTAAATATTAGAAAAATAGTAGCAATTTTTTAAAAAATTTTTAATTTTAATACCTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTaaa                                                                                  <  2:5437532/128‑3 (MQ=255)
                                              aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                                 <  1:4422038/128‑1 (MQ=255)
                                                cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                               <  2:4629322/128‑1 (MQ=255)
                                                   ttAATAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                            <  2:39199/128‑1 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATAAGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCTAATCATTATCTATTaa                                                                            >  2:2900133/1‑128 (MQ=255)
                                                    tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                           >  1:3864749/1‑128 (MQ=255)
                                                       aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTGTTAAATTATATTCAAATCATTAACTATTAATCaa                                                                        >  2:5272501/1‑128 (MQ=255)
                                                        aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                                       >  2:1397145/1‑128 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                     <  2:5010947/128‑1 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                     <  2:3118548/128‑1 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                    <  1:5024013/128‑1 (MQ=255)
                                                           ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                                    <  1:2673036/128‑1 (MQ=255)
                                                            taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTa                                                                   >  1:2476912/1‑128 (MQ=255)
                                                              ataCTTTTATTATTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATAATCTATTAATCAATTTTATg                                                                 <  1:3722435/128‑1 (MQ=255)
                                                                  ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATCg                                                           >  1:5443785/1‑128 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑t                                                        >  2:1984742/1‑128 (MQ=255)
                                                                    ttattaGTTGATATATTAGAAAACTACTAGCACTATTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAATATCTATTAATCAATTTTATGCTCt‑‑tt                                                       >  1:3415504/1‑128 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATCG‑‑ttt                                                      >  1:1201164/1‑128 (MQ=255)
                                                                      attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttt                                                     >  2:4367590/1‑128 (MQ=255)
                                                                           ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttt                                              >  1:5513949/1‑128 (MQ=255)
                                                                              atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑‑tttttttttttt                                           >  1:2766250/1‑128 (MQ=255)
                                                                                tttttATAAAACTACTATCACTTTTTTAAAAAATTTTTTATTTTAAAAAATGTATTATTTTTTAAATTTTTAAATTTTTTTTAAAAAATTATAAATTTATAAAATTTTTTGTCG‑‑tttttttttttttt                                        <  2:3555039/125‑1 (MQ=255)
                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttt                                        >  1:4339199/1‑128 (MQ=255)
                                                                                 tattttaaaaATACTATCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTATTTTAAAATAATTATATATTAATCAAATTTATGCTCG‑‑ttttttttttttttt                                       <  1:1429147/122‑1 (MQ=255)
                                                                                 taatataaaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTTTTTTTCAAATTTTTAAATTATTTTCAAATCAATATATATTAATCAATTTTATGCTCG‑‑ttttttttttttttt                                       <  1:4902770/122‑1 (MQ=255)
                                                                                  atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttttt                                      >  1:1951581/1‑128 (MQ=255)
                                                                                  atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttttt                                      >  1:2134918/1‑128 (MQ=255)
                                                                                    tAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑tttttttttttttttttt                                    >  1:2316527/1‑127 (MQ=255)
                                                                                     aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑ttttttttttttttttttt                                   >  1:4255752/1‑126 (MQ=255)
                                                                                     aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTaa                                  >  2:3268353/1‑127 (MQ=255)
                                                                                       aaaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCt‑‑tttttttttttttttttttta                                 >  2:2791887/1‑124 (MQ=255)
                                                                                          actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTACAAAAat                            >  2:4684789/1‑128 (MQ=255)
                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTTTaaaaaa                            >  1:5175354/1‑127 (MQ=255)
                                                                                           ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAat                            >  2:4475858/1‑128 (MQ=255)
                                                                                           ataatttcaattttttAAAAAAATTTTAATTTTAATAAATTTATTATTTTTAAAATTTTTTAAATTTTTTAAAATAATTATAAATTAATAAATTTTATGCTCTT‑‑TTTTTTTTTTTTTTTTTACaaaaa                             <  1:869926/118‑1 (MQ=255)
                                                                                            tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCT‑‑TTTTTTTTATTTTTTTTTAaaaaaaa                            >  1:5338572/1‑127 (MQ=255)
                                                                                              ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTTTAAAAAaata                         >  1:1801404/1‑128 (MQ=255)
                                                                                               tatatattttttTAAAAATTTTTAATTTTAATAAATTTATTATTTTTTAAATTTTTAAATTATTTTAAAATAATTTTTTATTTATAAATTTTATGTTTG‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:5513949/122‑1 (MQ=255)
                                                                                               taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTATATTTTTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTTTAAAAattaaa                        >  2:4274182/1‑124 (MQ=255)
                                                                                                 taaattttttAAAAAATTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATCTATTAATAAATTTTATTCTCTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAat                       <  1:3040290/124‑1 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTAG‑‑TTTTTTTTTTTTATTTTAACAAAAAaataat                       >  1:3546279/1‑128 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTAAAATAATTTTCAAATCATTATCTATTAATCAATTTTATGATCG‑‑TTTTTTATATTATTTTTTAAAAAAATATAat                       >  1:3119180/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACAAAAATATAatt                      >  1:77293/1‑128 (MQ=255)
                                                                                                   aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATAattaa                    >  1:1682010/1‑126 (MQ=255)
                                                                                                    tttttttAAAATATTTTTAAATATAATAAATTTATTATTTTTTAAATTTTTAAATTTTTTTAAAATAATTATATATTAATTAATTTTATTATATT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAattta                    <  1:2571648/127‑1 (MQ=255)
                                                                                                    cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                               <  1:1096192/92‑1 (MQ=255)
                                                                                                    cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAataatatattt                   >  2:1721026/1‑120 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATAatttat                   >  2:4853119/1‑128 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAAAAAATTAATTTTTTTTAAAATTTTTAAATTTTTTTAAAATAATTAAATATTAATAAATTTTATTCTCGTTTTTTTTTTTTTTTTTTACAAAAATATAatttat                   <  1:680893/128‑1 (MQ=255)
                                                                                                      tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATTAAATTTATTTg                >  2:419602/1‑128 (MQ=255)
                                                                                                        tttAAAAAATTTTTAATTTTTAAAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTATGCT‑‑TGTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  2:5204536/128‑1 (MQ=255)
                                                                                                        tttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATAatttatttt                >  2:4931442/1‑127 (MQ=255)
                                                                                                          tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAATAAAATttattttatt             >  1:1500413/1‑128 (MQ=255)
                                                                                                          nnnnanaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATAATttattttatt             >  2:2310191/5‑128 (MQ=255)
                                                                                                             aaaaTTTTTAATTTTAATAACTTAATTTTTTTTTAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTTTTCTCGTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaa           <  1:399143/128‑1 (MQ=255)
                                                                                                             aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTTTTttatta            >  1:2942866/1‑128 (MQ=255)
                                                                                                             aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATATTTTATTTTATTaa           >  1:1276398/1‑128 (MQ=255)
                                                                                                              aaaTTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAAATATTTTAAAATAATTATATATTAATCAATTTTATGTTTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAc         <  2:1631905/128‑1 (MQ=255)
                                                                                                              aaaTTTTTAATTTTAATAAATGAATTATTTTTGAAATTGTTAAAATATTTTCAAATAATTATATATTAATCAATTTTATTCTCG‑‑TTGTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAc         <  2:509539/128‑1 (MQ=255)
                                                                                                               aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTTTTTTATTAAAc         >  2:4756451/1‑128 (MQ=255)
                                                                                                               aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTAAAAAAAATAAATATTATTTTATTAAAc         >  2:595063/1‑128 (MQ=255)
                                                                                                                          ||                                                                                                                                 
CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420041‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: