Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681634,9451.A44.1% 126.8 / inf 1466intergenic (+49/+117)JCVSYN2_00105/JCVSYN2_00110ABC transporter permease/cell division protein FtsH
Reads supporting (aligned to +/- strand):  ref base . (293/525);  new base A (599/47);  total (893/573)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-20

Only 100 of 1658 total aligned reads displayed.

CTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGT  >  CP016816/34818‑35069
                                                                                                                                      |                                                                                                                                      
  ttACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑aa                                                                                                                                    >  1:1002043/1‑128 (MQ=255)
  ttACTTATTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGCTTTTATTATTTACTATT‑‑T‑‑‑aa                                                                                                                                    >  1:2658968/1‑128 (MQ=255)
      ttcttTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAATATTAAACCAAATACAATGTATATTTTTTTTTTATTTAATATT‑‑T‑‑‑aaaaaa                                                                                                                              >  2:1732057/1‑128 (MQ=255)
            aTGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATGATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑aaaaaaaaaaaa                                                                                                                      >  1:3182009/1‑128 (MQ=255)
              ttcctttataaaaaaTAGTAATAAATTCTTAACCAATTAAAATTCTAAGTTAAGTTTATTAATAATAAAATATTAAACAAAATAAATTGTGTTTGGTTTTTTTATTTAATATT‑‑T‑‑‑aaaaaaaaaaaaaa                                                                                                                    <  2:460239/118‑1 (MQ=255)
              gAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGATAAGTGAAGTTGATGAATAATAAAATATTAAACCAAATACATTGTATTTGTTTTTTTTATTTACTATT‑‑T‑‑‑aaaaaaaaaaaaaa                                                                                                                    >  1:522522/1‑128 (MQ=255)
               tttttAAACAAAAATAGTATGAAATTCTGAACCAAGTAAAATGATAAGTTAATTTGTTTAATAAAAAAACATTAAACCAAAAAAATTGTTTTTGGTTTTTTTATTAACTATT‑‑G‑‑‑aaaaaaaaaaaaaaa                                                                                                                   <  1:3316037/126‑1 (MQ=255)
               tgTTTCAAAAAAAACTTTAATAAATGATTAACCTATTAAAATCGTAATTTAATTTGATTAAAAAAAAAATTTTAAACAAAAAAAATTTTGTTTGGTTTTTTTATTTAATATT‑‑T‑‑‑aaaaaaaaaaaaaaa                                                                                                                   <  2:289792/127‑1 (MQ=255)
                 tttCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑aaaaaaaaaaaaaaaaa                                                                                                                 >  1:3864848/1‑126 (MQ=255)
                   tCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAataata                                                                                                              >  1:3417388/1‑128 (MQ=255)
                    caacaaAAAGAGTAAGAAATGCTGAACCAAGTACAATTCTAAGTTAAGTTTATGAATAATAAAAGATTAAACCAAATACATAATAATTATTTTTTTTATATACAATA‑‑G‑‑‑aaaaaaaaaaaaaaaaaaaa                                                                                                              >  1:2905161/1‑126 (MQ=255)
                    caacaaAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑aaaaaaaaaaaaaaaataat                                                                                                              >  1:3963728/1‑123 (MQ=255)
                        aaaaaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTTGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAATAATA‑‑TAAtt                                                                                                       >  1:1981260/1‑128 (MQ=255)
                          aaaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAATATTAAACCAAAAACATTAATTATAATAATATTATTTACAATa‑‑a‑‑‑aaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                        >  2:1641311/1‑120 (MQ=255)
                           aaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAaaaataatataaa                                                                                                       >  1:3009627/1‑121 (MQ=255)
                               gTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑T‑‑‑AAAAAAAAAAAAAAAAAAAA‑‑AAAttttaaaa                                                                                                 >  2:4028874/1‑123 (MQ=255)
                               gTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAAAA‑‑TAAtttaaa                                                                                                   >  1:3364759/1‑125 (MQ=255)
                                  ataaaTGAGGAACCAATTACAATGCTAATTGAAGTTTTTTAATAATAAAATATTAAACCAAAAACATTGTGTTTGGTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACt                                                                                              <  2:3284869/126‑1 (MQ=255)
                                   gAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATA‑‑TAATTTTAATAatat                                                                                            >  1:1444937/1‑128 (MQ=255)
                                    aaaTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACtaaa                                                                                           >  2:3768391/1‑126 (MQ=255)
                                    aaaTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAAAA‑‑TAATTTAAATAAtata                                                                                           >  2:3332071/1‑128 (MQ=255)
                                     aattcttacccAATTACAATGGTATGTGTATTTTTTTAAAAATAAAATATTAAACCAAATACATTGTTTTTGGTTTTTTTATTTACATTTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACtata                                                                                           <  1:326945/119‑1 (MQ=255)
                                     aaTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATaa                                                                                          >  1:1077575/1‑128 (MQ=255)
                                      aTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATA‑‑TAATTTGCATAATATaaa                                                                                         >  1:4006129/1‑128 (MQ=255)
                                        gCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑T‑‑‑AAAAAAAAAAAAAAAAAAAA‑‑AAATTTTAAAAATATaaaaa                                                                                       >  1:2946755/1‑128 (MQ=255)
                                        gCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATA‑‑TAATTTGCAtaaaataaaa                                                                                        >  1:457956/1‑128 (MQ=255)
                                           taaaaaaGTAAAATGATATTTGAATTTTATTAATAATAAAATATTAAAAAAAATAAATTTTGTTTGGTTTTTTTTTTTACTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAAt                                                                                     <  1:1370511/123‑1 (MQ=255)
                                             aCCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGTTTTTTTTATTTACTATTAA‑‑‑AAAAAAAAAAAAAAATAATA‑‑AAATTAGCATAATATAAAAAAttt                                                                                   >  1:2445758/1‑127 (MQ=255)
                                                  gTACAATGCTAAGTGAATTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTAATATT‑‑G‑‑‑AAGAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGttttt                                                                              <  2:1391593/128‑1 (MQ=255)
                                                   tACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTTCATAAAAAAAAAAAATtttttta                                                                             >  2:846767/1‑127 (MQ=255)
                                                      aatgttattttaaGTTGTTAAATATTAAAATATTAAAACAAATAAATTTTTTTTGTTTTTTTTATTTAATTTTTAA‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCaa                                                                           <  2:3467768/117‑1 (MQ=255)
                                                      aaTGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATA‑‑AAATTTGAATAATATAAAAAATttttttaaaa                                                                           >  1:3114557/1‑124 (MQ=255)
                                                      aaTGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAAAA‑‑AAATTTTAATAAATTAAAAAAATTTTTTTaaaa                                                                          >  1:1290327/1‑128 (MQ=255)
                                                       aTGCAAAGTTTATTTTATTAAAAATAAAATAATAAACCAAATAAATTTTTTTTGGTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAAt                                                                         <  2:1304261/128‑1 (MQ=255)
                                                        tGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAAAA‑‑AATTTTGAATAATAAAAAAAATTTTTTTTaaaaa                                                                         >  2:3976578/1‑127 (MQ=255)
                                                        tGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATATTTATTTTGCATAAAATAAAAAATTGTTTTTAAAAt                                                                         >  1:1706850/1‑128 (MQ=255)
                                                         gCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAAAA‑‑TAATTTCAAAAAAATAAAAAATTTTTTTACaaaaaa                                                                       >  2:2112952/1‑128 (MQ=255)
                                                            aaGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAAATTTAATAATATAAAAAATttttttaaaaaaaataa                                                                    >  1:2345932/1‑117 (MQ=255)
                                                             aGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAAAA‑‑TAATTTTCAAACTATAAAAAATTATTTTTCAAATAAGTAt                                                                   >  2:3406297/1‑128 (MQ=255)
                                                              gTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGAATACTAAAAAAAAATGTATTTAAAATAattatt                                                                  >  1:95770/1‑128 (MQ=255)
                                                                gatttttattaataaaAAAATATTAAAACAAATAAATTGTGTTTTGTTTTTTTATTTAATATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAg                                                               <  1:1543384/118‑1 (MQ=255)
                                                                  tttttattaaaaataaAATATTAAACCAAATAAATTGTGTTTGGTTTTTTTATTTAATATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGa                                                             <  2:3835128/117‑1 (MQ=255)
                                                                    ttGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTATATTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAAAA‑‑TAATTTGCTTACTATAAAAAATTGATTTAAAAAAAATTATAAAGGAg                                                            >  1:3601167/1‑128 (MQ=255)
                                                                     tgatgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAAAA‑‑TAATTTTAATAATAAAAAAAATTTTTTTTAAAATAattattaataaata                                                          >  2:1516530/1‑120 (MQ=255)
                                                                       atgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGAATAATATAAAAAATTTTTTTTAAAAAAATTATTaagaagaat                                                         >  1:870518/1‑125 (MQ=255)
                                                                        tgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATA‑‑TAATTTAAATAAAAAAAAAAATTATTTTAAAAAAAATAATTCaaaattaa                                                         >  1:2813085/1‑121 (MQ=255)
                                                                          attttaatattaTTAAAACAAATAAATTTGTTTTTGTTTTTTTATTTAAATTTTAA‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAAt                                                      <  1:2144753/117‑1 (MQ=255)
                                                                             aataaAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGAATAATATAAAAAATTTTTTTTAAAATATTTATTAATTATTAAATTTaa                                                  >  1:3810553/1‑128 (MQ=255)
                                                                             aataaAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGAATAATATAAAAAATAGTTTTTAAATTAAGTATTAATTATTAAATTTaa                                                  >  1:3524014/1‑128 (MQ=255)
                                                                                     aTTAAAAAAAATAATTTTTGTTTGGTTTTTTTTTTTAATTTT‑‑T‑‑‑AAAAAAAATAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAag                                         <  1:2503015/128‑1 (MQ=255)
                                                                                      ttAAACAAAATACATTGTTGTTGGTGTTTTTTTTTACTATT‑‑T‑‑‑AAATAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAag                                         <  1:3566256/128‑1 (MQ=255)
                                                                                      ttAAAAAAAAAAAATTTTTTTTGGTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAag                                         <  2:318161/128‑1 (MQ=255)
                                                                                       tAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTTGATAATTTAAAAAATTTTTTTTAAAATAATTATTAATGATTTAATTTAAAAAACaaaaa                                        >  1:3742609/1‑126 (MQ=255)
                                                                                        aaaCCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAAAAAATT‑‑TAATTTCAATAAAAAAAAAAATTTTTTTTAAAAAAATTAAAAATAATTAAAATTAAAAAACaaaa                                         >  2:2506376/1‑127 (MQ=255)
                                                                                        aaaCCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTCAATAATATAAAAAATTTTTTTAAAAATAATTATAAAGTATTAAATTTAAAAAACaaatat                                       >  2:3897217/1‑125 (MQ=255)
                                                                                        aaaCCAAATACAATGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATAATATAAAAAATTTATTTTAAAAAAATTATAAAGAATTAAATATAAAAAACAAaaag                                       >  1:676271/1‑128 (MQ=255)
                                                                                          aacataTACATTTTTTTTGGTTTTTTTTTTTTATATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTta                                    <  2:1940571/123‑1 (MQ=255)
                                                                                           ccAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATA‑‑AAATTTGAAAAATAAAAAAAATTTTTTTAAAAATAATTTTACACATTAAAATTTaaaaaaaaaatattaa                                    >  1:2586465/1‑118 (MQ=255)
                                                                                           ccAAATAAATTGTGTTTTGTTTTTTTATTTACTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTta                                    <  2:753121/128‑1 (MQ=255)
                                                                                           acAAATAATTTGTGTTTGTTTTTTTTATTTACTTTT‑‑G‑‑‑TAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                  <  2:651931/127‑1 (MQ=255)
                                                                                            cAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAAAA‑‑AAATTTGACAAAAAAAAAAAAATTTTTTTAAAAAAAAAATAAAGAATTAAATTAAAAAAACCAAAAGTtat                                   >  2:3703390/1‑128 (MQ=255)
                                                                                            aaattACATTTTGTTTGGTTTTTTTATTTAATATTTAA‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTGAATTTAAAAAACCAAGATTta                                    <  1:1904296/124‑1 (MQ=255)
                                                                                             aaataaatTGTGTTTGGTTTTTTTTTTTACTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                  <  2:1836218/128‑1 (MQ=255)
                                                                                             aaaTATATTGGGTTTGGTTTTTTTATTTATTATT‑‑GA‑‑AAAAAAAAAAAAAAATAAAA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTAATCAGGAGTAAATTTAAAAAACCAAGAGTtata                                  <  2:2062716/128‑1 (MQ=255)
                                                                                             aaaTACATTGTGTTTTTTTTTTTTTTTTATTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                  <  2:280688/128‑1 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAAAA‑‑TAATTTTCAAAAAATAAAAAATTTTTTTTAAAAAAATAATAAAAGATTAAATTTAAAAAACAAATATTtata                                  >  1:812205/1‑128 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATT‑‑TAATTTGCATAATAAAAAAAATTTTTTTTAAATAAATTATAAATAATTAAATTTAAAAAACAATTATTTATaa                                 >  1:346591/1‑128 (MQ=255)
                                                                                              aatattttGTGTTTGGTTTTTTTTTTTAATATTTAA‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                  <  2:3078838/122‑1 (MQ=255)
                                                                                              aaTACATTGTGTTTGGTTTTTTTATTTACTATA‑‑G‑‑‑AAAAAATAAAAAAAAATAAAA‑‑TAATTTGAATACTATAAAAAATTGATTTTAAAATAATTATTCACTAGTAAAATTTaaaaaaaatgatttaata                                  >  1:181052/1‑116 (MQ=255)
                                                                                               ttaaaTTGTTTTTGTTTTTTTTTTTTATTATTTAA‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTTATaa                                 <  2:582256/124‑1 (MQ=255)
                                                                                               atattttgttttttgttttTTTATTTTATTTTT‑‑AAAAAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                  <  2:2207075/123‑1 (MQ=255)
                                                                                                taaattttttttGGTTTTTTTATTTAAATTT‑‑T‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATc                              <  1:17663/119‑1 (MQ=255)
                                                                                                tACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAAAAAAA‑‑TAATTTGAATAATATAAAAAAATTTTTTTCAAATAAGTATAAGGGAGTAATTTAAAAAAACCAAGAGTtaataa                                >  1:2093160/1‑124 (MQ=255)
                                                                                                  tatttgttttGGTTTTTTTTTTTACAATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCt                             <  2:2648673/121‑1 (MQ=255)
                                                                                                   aTTTTGTTTGGTTTTTTTATTTAAAATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTTATAACTCtt                            <  2:3625368/128‑1 (MQ=255)
                                                                                                   aTTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATA‑‑TATTTTACATAATATAAAAAATTGTTTTTAAAAAAAGTATTCCGGAGTAAATTTAAAAAAAATAGAGTTatatatat                             >  1:2007887/1‑126 (MQ=255)
                                                                                                    ttttttttttttttttttttttttttt‑‑t‑‑‑TAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTgg                          <  2:2333200/118‑1 (MQ=255)
                                                                                                    ttGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑AAATTTGAATACTATAAAAAATTTTTTTTAAAAAAACTATTCAGTAGTAAATTTAAAAAACCAATAATTATAAATATTg                           >  2:3011376/1‑128 (MQ=255)
                                                                                                    atgtaTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAAAA‑‑TAATTTGCATACAAAAAAAAATTGTTTTTCAAATAAGTATTAAGTAGTAAATTAAAAAAACAAAAAGTTAAAAAACTTg                           >  2:2726979/2‑128 (MQ=255)
                                                                                                     tgtgTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑AAATATTAATAAAATAAAAAATTTTTTTTAAAATAATTATTATTAATTAAATTAAAAAAAACAATATTTAaaaaaattgt                          >  2:243401/1‑122 (MQ=255)
                                                                                                     tgtgTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATA‑‑TAATTTGCATAATTTAAAAAATTGTTTTTAAAATAATTATTCAGTATAAAATTTAAAAAACAAATAGTTATAAATATTgt                          >  1:1154614/1‑127 (MQ=255)
                                                                                                     tgtaTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATA‑‑TAATTTGAATAATATAAAAAATTTTTTTAAAAATAATAATTAATTAATAAATTTAAAAAACAAAGAATTATAATTATTgg                          >  1:1630439/1‑128 (MQ=255)
                                                                                                      gtatttGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATAATAAAAAAAATTTTTTTTAAAATAAGTATTAAGGATTAAATTTAAAAAACCAAAATTTATAAATCTTGGt                         >  2:929833/4‑128 (MQ=255)
                                                                                                       tatttGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAAAA‑‑AAAATTGCATACAATAAAAAATGTTTTTTCAAATAAGTATTCCGGAGTAAATTTAAAAAACCAAGCGTGATAAAACATGGtag                       >  2:2276967/3‑126 (MQ=255)
                                                                                                         tttGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATA‑‑TAATTTGAATAATATAAAAAATTGTTTTTCAAAAAATTATTATTTTGTAAATTTAAAAAACAAATATTTATAAATAttttttt                       >  2:3560083/1‑123 (MQ=255)
                                                                                                         tttGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAAAA‑‑TAATATACAAAAAATAAAAATTATTTTTTAAAATAATAAATCATAAGAAAATTTAAAAAAAAAATATTTATAAAAATTTGtttt                      >  1:272732/1‑128 (MQ=255)
                                                                                                          ttGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATA‑‑TAATTTTCAAAATATAAAAAATTGTTTTTCAAATAATTTTTAATTATTAAATTTAAAACACAAAAATATATATATcatagatat                      >  2:2000758/1‑120 (MQ=255)
                                                                                                          ttGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TATTTTGAATAATATAAAAAATTTTTTTTAAAATATTTATAAATGATTAAATTTAAAAAACAATAAGTTATAAATATTGtttttt                     >  1:597545/1‑128 (MQ=255)
                                                                                                           tGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAAAAATA‑‑TAATTTAAAAAAAAAAAAAAATTTTTTTAAAAATAATTATAAAGTATAAAATTAAAAAAACAAAAATTTAAAAATTTTGtttttt                     >  2:1211422/1‑128 (MQ=255)
                                                                                                              tttttttATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAAA‑‑AAATTTGCATAATATAAAAAATTTTTTTTAAAATAATTTTTAATGTTTTAATTTTAAAAACCAACATTTAAAAATAATGTTTTTTTTTTta               >  1:3697264/1‑128 (MQ=255)
                                                                                                               ttttttATTTTATATT‑‑T‑‑‑AAAAAAATAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGttt                <  2:3524014/128‑1 (MQ=255)
                                                                                                               ttttttATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGttt                <  1:3063488/128‑1 (MQ=255)
                                                                                                                tttttATTTAATTATT‑‑AA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGttt                <  2:1056549/128‑1 (MQ=255)
                                                                                                                 ttttATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTTAATAATATAAAAAATTTTTTTTAAAATAAATATTAATTATTAAATTTAAAAAACAAAGATTTATAAATATTGTTTTTTTGTTtat              >  1:2272361/1‑128 (MQ=255)
                                                                                                                 ttttATTTACTATT‑‑‑‑‑TAAAAAAAAAAAAATAATA‑‑TAATTTTCAAAATATAAAAAATTTTTTTTAAAATAATTATTAAGGATTAAATTTAAAAAACCAAGAGTTATAAATATTTGTTTTTTTTTTAtatt           >  2:1148665/1‑128 (MQ=255)
                                                                                                                    tatttaCTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑AAATTTGCATAATATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAACATTTATAAAACTTGTTTTTTTGTTTAtatta          >  2:2591317/1‑128 (MQ=255)
                                                                                                                     atttaCTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATAATATAAAAAATTGTTTTTCAAATAATTATTCAGGATTAAATTTAAAAAACAAAGATTTATAAATATTGTTTTTTTGTTTAtattat         >  1:739494/1‑128 (MQ=255)
                                                                                                                           aCTATT‑‑G‑‑‑AAAAAAAAAAAAAATAATA‑‑TAATTTGAATACTATAAAAAATTGTTTTTCAAATAATTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTAttt    >  1:3910813/1‑128 (MQ=255)
                                                                                                                              tATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTg   <  1:2211579/128‑1 (MQ=255)
                                                                                                                               aTT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGt  <  1:1215369/128‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATA‑‑TAATTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGT  >  CP016816/34818‑35069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: