Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT98.1% 4036.3 / ‑1.3 1365intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (4/22);  new base T (807/531);  total (812/553)
Fisher's exact test for biased strand distribution p-value = 5.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1415 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346
                                                                                                                              |                                                                                                                                
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                             <  2:2781036/128‑3 (MQ=255)
    atttAATTGTATTAATTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                            <  2:3591581/127‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                           >  1:1921294/1‑128 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                           >  1:1670973/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                         <  1:1008035/128‑1 (MQ=255)
        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                        <  1:942656/128‑1 (MQ=255)
        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                        >  1:363030/1‑128 (MQ=255)
            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                    >  2:3461948/1‑128 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                   <  1:3152680/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  >  2:1159728/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  >  1:801564/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:3101562/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:1546577/128‑1 (MQ=255)
               ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                 >  1:2135210/1‑128 (MQ=255)
                tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACt                                                                                                                <  2:1312015/128‑1 (MQ=255)
                  ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                              <  2:2955898/128‑1 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATAATTTTTTTATATGCAAATTTATAAAAAATTAATTTGAACTTGTa                                                                                                            >  1:874177/1‑128 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATat                                                                                                    >  1:536689/1‑127 (MQ=255)
                             gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                   >  1:1291136/1‑128 (MQ=255)
                             gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                   >  1:3469730/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttt                                                                                                >  1:2213701/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                >  1:2670828/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                               >  2:866652/1‑128 (MQ=255)
                                    tttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttt                                                                                            >  2:2380036/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                        >  2:286094/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttattttt                                                                                        >  2:560927/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttt                                                                                       >  1:602515/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                     >  2:3768986/1‑128 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtttttttttttttttac                                                                                  <  1:3026097/128‑3 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttt                                                                                  >  2:1518664/1‑128 (MQ=255)
                                                  tttATTGCACTTGTATGAAATAGTAACAAACAAAAAATATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCTTTTTTTTTTTTTTTTtataa                                                                              <  1:2592713/128‑2 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataa                                                                              >  1:751350/1‑127 (MQ=255)
                                                    tatatcACTTTTATGAATTATTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTtataatg                                                                            <  1:2272246/123‑4 (MQ=255)
                                                     ttCGCATTTTTATGAATTATTAATAAAATAAAAATTTTTTTTTTTTTTATATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                           <  2:3506072/127‑5 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataatttaaa                                                                         >  1:468546/1‑118 (MQ=255)
                                                         cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaatgtaaat                                                                       >  2:1765067/1‑118 (MQ=255)
                                                            attatttaaTTTGTAATAAACTTAAAATATTATATTTTTATTTATTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                  <  2:2286812/121‑1 (MQ=255)
                                                               ttttaaTTATTAAGAAAAAAAAAATATTATCTTTTTATATAATTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                               <  2:3055160/124‑1 (MQ=255)
                                                               tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATATAAAt                                                               >  2:1012859/1‑128 (MQ=255)
                                                                 tGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAAAATAAAttt                                                             >  1:3937463/1‑128 (MQ=255)
                                                                   aaattctAAGTAACTAAAAATAATATATTTTTATATATTTTTTGTTAATCCAAATTTTTAAAAAATTAATTGTAACTTGTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                           <  2:1991413/122‑1 (MQ=255)
                                                                    atttttaataaAATAAAAATATTATATTTTTTGTTTTTTTTTTTAAATTAAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                          <  2:3409215/123‑1 (MQ=255)
                                                                      tagtaatatcataaaacttTTATATTTTTTTATATTTTTTGTATAAGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                        <  2:2074035/117‑1 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:1402728/72‑1 (MQ=255)
                                                                        tttataaactaaaaattTTTTATTTTTATTTATTTTTTTTATATGCAAATTTTTAAAAAAATAATTTTTACTTGTAAAAAATACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                      <  1:3518611/123‑1 (MQ=255)
                                                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTtatttatt                                                     >  1:3359421/1‑128 (MQ=255)
                                                                        gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGAAAATATTATTTTTattta                                                       >  1:3996528/1‑124 (MQ=255)
                                                                         taataaAATAAAAATATTTTTTTTTTATTTTTTTTTTGTAATTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAG‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtt                                                     <  1:3576810/128‑1 (MQ=255)
                                                                          aaGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTCTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATTAATTTtatttattat                                                   >  2:1904180/1‑126 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATttttttt                                               >  1:2085664/1‑127 (MQ=255)
                                                                             aaaataaaaattTTTTATTTTTTTATTTTTTTTTTTTATTTAAATTTTTAAAAAATTTATTTTAAATTTTATAAAATTTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                  <  1:654049/128‑1 (MQ=255)
                                                                              taataaaaataTTATTTTTTTTTTATTTTTTTTTATATTTAAATTTTTAAAAAAATAAATTTTAATTTTATAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  1:693005/125‑1 (MQ=255)
                                                                              aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTATTTTTTTTTTTAAAATTTAAATATAAATTTTAGttttttttttt                                               >  2:2123505/1‑127 (MQ=255)
                                                                                ataaaaattTTATATTTTTTTATTTTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  2:1854155/127‑1 (MQ=255)
                                                                                ataaaaataTTATTTTTTTTTATATTTTTTTTTTATTAAAATTTTTAAAAAATTTATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgttg                                               <  2:4023946/127‑1 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTATTTATtttttttaa                                             >  1:1053436/1‑125 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATAAAATTTTTAGTTATttttttttaa                                            >  2:927433/1‑124 (MQ=255)
                                                                                 taaaaataTTATTATTTTTTTTAATTTTTTTATATTTAAATTTTTTAAAAAATAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTc                                             <  1:291544/128‑1 (MQ=255)
                                                                                  aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAAAGTAAATATAAATTTAATTAATTTTTTTTTCTa                                           >  1:411046/1‑128 (MQ=255)
                                                                                  aaaaataTTATATTTTTATATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCt                                            <  1:1450356/128‑1 (MQ=255)
                                                                                     agatttatATTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTTAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  1:3790435/124‑1 (MQ=255)
                                                                                     aatattatCTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  2:1441409/126‑1 (MQ=255)
                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGGAAATAAAATTTTATGTttttttttttaaatctt                                       >  1:2006344/1‑119 (MQ=255)
                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTAAGTTATTTTTTTTTATAATTa                                       >  2:691521/1‑128 (MQ=255)
                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTATAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATTATGAAAAAATAATTTTTATATTTTtttttgaataa                                          >  2:1650800/1‑123 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATAAAATTAAAATTTTAAGTATtttttttttaatat                                        >  2:460371/1‑120 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATttttttttatattta                                       >  1:1250719/1‑119 (MQ=255)
                                                                                       tattatATTTTTTTATATTTTTTGTAAATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                     <  2:3684429/128‑1 (MQ=255)
                                                                                        attatCTTTTTAGATATTTTTTGTATTATAAAATTTTTAAAAAATTAATTTGAACTTTTAAAAATTA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      <  1:1763139/128‑1 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTGTATAATTAta                                     >  1:2796225/1‑127 (MQ=255)
                                                                                          tttttttttATTTATTTTTTTTATATTTAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:1589530/124‑1 (MQ=255)
                                                                                          tatCTTTTTTTTTATTTTTTTTATATTATAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:1145793/128‑1 (MQ=255)
                                                                                           atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTTGTATTTTGGTGTCTAATTATCTa                                   >  2:2714898/1‑128 (MQ=255)
                                                                                              tttttAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTCTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                               <  1:2415790/128‑1 (MQ=255)
                                                                                                tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTGTTTTATAATTATATaaaaaaa                             >  1:3279309/1‑128 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTTTTTTGTTGTATAAGTATCTaaaaaaa                             >  2:609198/1‑128 (MQ=255)
                                                                                                 ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑‑‑TCTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  1:133154/128‑1 (MQ=255)
                                                                                                  tAGATATATTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAG‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                           <  1:270607/128‑1 (MQ=255)
                                                                                                   ttttatTTTTTGTATATTAAAATTTTTAAAAAATTAAATTTAACTTGTTAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  1:3698915/125‑1 (MQ=255)
                                                                                                   atatatTTTTTGTATATGAAAATTTTTAAAAAATTAATTTGTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  2:3252822/126‑1 (MQ=255)
                                                                                                   aGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAATTAg                          >  1:2319456/1‑128 (MQ=255)
                                                                                                    tttatTTTTTTTTTTTTGAAATTTTTTAAAAATTAATTTTTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                         <  1:1835104/126‑1 (MQ=255)
                                                                                                    gATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAAGTTATTTTTTTGTCTAATTATCTAAAAAAATaaa                         >  1:3422750/1‑126 (MQ=255)
                                                                                                     atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTATAGATATTTTTTTGTTTAAGTATATAAAAAAATATaa                        >  2:2583640/1‑128 (MQ=255)
                                                                                                       atTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                       <  2:1378374/128‑1 (MQ=255)
                                                                                                       atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTAAAATTATAAAAAAAAATATaaa                       >  2:776089/1‑128 (MQ=255)
                                                                                                         tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAAAAAAAATTTAATTTATTTTTTTTTCTAATAATAAAAAAAAATATaaatt                     >  1:2853060/1‑126 (MQ=255)
                                                                                                          ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTGTATAATTATCTAAAAAAATATAAAGTaa                   >  2:2222182/1‑128 (MQ=255)
                                                                                                           tttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTAAAATTATATAAAAAAATATaattaaa                   >  2:3925638/1‑123 (MQ=255)
                                                                                                            ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAAAATAAATTTTATTTATTTTTTTTTATAATTATATAaaaaaaaaaaaaataa                   >  2:1253457/1‑124 (MQ=255)
                                                                                                             tgtttttttaaaTTTTTAAAAAATTATATTGTAATTGTTAAAAAATTGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                 <  2:3137705/119‑1 (MQ=255)
                                                                                                             tgtttttGCAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                  <  2:3203045/122‑1 (MQ=255)
                                                                                                             tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATATAAAAAAATAGAAAGGaataa                >  1:2477976/1‑128 (MQ=255)
                                                                                                              ttataTTTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaaa               <  1:3860956/127‑1 (MQ=255)
                                                                                                                atttgaaaaTTTTTAAAAAATTTATTTGAAGTTTTAAAAAA‑‑‑‑TTGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat            <  2:2499724/122‑1 (MQ=255)
                                                                                                                atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATGTATTTTTTTTTATAAGTATATAAAAAAATAAAAAGTAAAAAat              >  2:3343672/1‑128 (MQ=255)
                                                                                                                      aaaTTTTTTAAAAAATAATTTTAAATTTTAAAAAATA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa       <  2:3869320/128‑1 (MQ=255)
                                                                                                                       aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATATAAAAAAATAGAAAGTAAAAAATATTTTTaa      >  2:107971/1‑128 (MQ=255)
                                                                                                                        aTTTTTTAAAAATTTATTTGAAAATTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa     <  2:1584550/128‑1 (MQ=255)
                                                                                                                           tttAAAAAAATTAATTTAACATTTAAAAAAA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt  <  1:3207880/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                                
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: