BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.8%	5604.4 / NA	1746	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (854/888); minor base A (4/0); total (858/888)
Fisher's exact test for biased strand distribution p-value = 5.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1766 total aligned reads displayed.

TATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281490‑281740

   gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                          <  1:328540/128‑1 (MQ=255)

   gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                          <  2:526623/128‑1 (MQ=255)

      tCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGATAAATAatatat                                                                                                                       >  1:3349921/1‑128 (MQ=255)

      tCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATaa                                                                                                                            <  1:1716436/123‑1 (MQ=255)

       cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGAAAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      <  2:928003/128‑1 (MQ=255)

         aTTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaa                                                                                                                    >  2:1004299/1‑128 (MQ=255)

             cTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGt                                                                                                                <  2:3251338/128‑1 (MQ=255)

              tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                               <  1:2314865/128‑1 (MQ=255)

                 aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGTATTTACTAATTTCTCTAATTAATAAAATTGATACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            >  1:4037527/1‑128 (MQ=255)

                 aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            <  1:284524/128‑1 (MQ=255)

                 aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            <  2:2240034/128‑1 (MQ=255)

                 aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAAAAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                            <  1:881298/128‑1 (MQ=255)

                  aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:2795839/128‑1 (MQ=255)

                  aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  1:1911180/128‑1 (MQ=255)

                  aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTATaaa                                                                                                           >  2:3835543/1‑128 (MQ=255)

                   cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                          <  2:1607556/128‑1 (MQ=255)

                   cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                          <  1:1172678/128‑1 (MQ=255)

                    tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         <  1:3057860/128‑1 (MQ=255)

                      gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       >  2:174040/1‑128 (MQ=255)

                      gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         <  1:1617303/126‑1 (MQ=255)

                           ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                  >  1:552130/1‑128 (MQ=255)

                             attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaa                                                                                                                     <  2:603014/107‑1 (MQ=255)

                             attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                >  2:3005161/1‑128 (MQ=255)

                              ttattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaa                                                                                                                    >  2:2183585/1‑107 (MQ=255)

                                  ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           <  2:2078778/128‑1 (MQ=255)

                                     atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAAGAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                        <  2:1540521/128‑1 (MQ=255)

                                     atttaGAGACAAAATTAAATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                        >  2:756593/1‑128 (MQ=255)

                                      tttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGt                                                                                       <  1:1360059/128‑1 (MQ=255)

                                              cAAAATTACATTTATAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  1:731138/128‑1 (MQ=255)

                                              cAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  2:1314444/128‑1 (MQ=255)

                                              cAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  2:3582516/128‑1 (MQ=255)

                                              cAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGa                                                                               <  1:1556934/128‑1 (MQ=255)

                                               aaaaTTACATTTAAAACAAAAACAGGATTTACTATTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAATTAATTTATAAAAACGTTAAAAGATGTGAGTTGATGATAGTATTAATGAt                                                                              >  2:1112714/1‑128 (MQ=255)

                                               aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGa                                                                                                   <  1:3417335/107‑1 (MQ=255)

                                               aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  2:2367171/1‑128 (MQ=255)

                                               aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  1:617590/1‑128 (MQ=255)

                                               aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  1:667241/1‑128 (MQ=255)

                                                aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATTAAAAGTTTAAAAGAGGTGAGTTGATGAAAGTTTTATTGATc                                                                             >  2:2531882/1‑128 (MQ=255)

                                                 aaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATct                                                                            >  1:2520624/1‑127 (MQ=255)

                                                    tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGgag                                                                         <  1:3976052/128‑1 (MQ=255)

                                                    tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGgag                                                                         >  2:807805/1‑128 (MQ=255)

                                                      cagttAAAACAAATCCCGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATTAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGAt                                                                       <  2:1181397/125‑1 (MQ=255)

                                                      cATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTCATGAAAGTATTAATGATCGGAGAt                                                                       >  1:3900979/1‑128 (MQ=255)

                                                       aTTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATg                                                                      <  1:359575/128‑1 (MQ=255)

                                                        tttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     <  2:3910617/128‑1 (MQ=255)

                                                         ttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGATAGTATTAATGATCGGAGATGtt                                                                    >  1:3527056/1‑128 (MQ=255)

                                                          tAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGttt                                                                   <  2:12614/128‑1 (MQ=255)

                                                           aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTATAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:2467506/1‑128 (MQ=255)

                                                           aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  <  2:486681/128‑1 (MQ=255)

                                                            aaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTAt                                                                 >  1:1798430/1‑128 (MQ=255)

                                                             aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAATAGTTGATTTGATGAAAGTATTAATGATCTGAGATGTTTATg                                                                >  2:885443/1‑128 (MQ=255)

                                                             aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                >  1:2672037/1‑128 (MQ=255)

                                                                aaaaacaTGATTTACTAATTTCTCTAATTAATAAAATTGAATATAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaa                                                             <  2:1462811/128‑1 (MQ=255)

                                                                 aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaaa                                                            <  1:1427432/128‑1 (MQ=255)

                                                                   ccgatcttttACTAATTTCTCTAATTAATAAAATTGAAACAAATAAAATAGATGAAAAATAATTTATAAAAAGTATAAAAGATGTGATTTGATGAAAGTATTAATGATCGGAGATGTATATCCaaatc                                                          >  2:2657608/8‑126 (MQ=255)

                                                                   aacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAc                                                          <  1:1854522/128‑1 (MQ=255)

                                                                   aacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAc                                                          <  1:125015/128‑1 (MQ=255)

                                                                    acaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAcc                                                         <  1:1077736/128‑1 (MQ=255)

                                                                      aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCgg                                                       >  2:4006337/1‑128 (MQ=255)

                                                                       ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAACATAATTTATAAAAAGTATAAAAGAGGTGATTTGATGAAAGTATTATTGATCGGAGATATTTATCAAAAAACgcg                                                      >  1:2191553/1‑126 (MQ=255)

                                                                       ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCggg                                                      <  1:2466120/128‑1 (MQ=255)

                                                                         aTTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGaa                                                    <  1:1304256/128‑1 (MQ=255)

                                                                         aTTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGaa                                                    >  2:2923046/1‑128 (MQ=255)

                                                                          tttACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAag                                                   <  2:3122721/128‑1 (MQ=255)

                                                                          tttACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATAAATGATCGGAGATGTTTATGCTAAACCGGGAag                                                   >  2:1674715/1‑128 (MQ=255)

                                                                           ttACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaaa                                                            <  1:2415671/118‑1 (MQ=255)

                                                                            tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAagag                                                 <  1:3225469/128‑1 (MQ=255)

                                                                             aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                <  1:2261463/128‑1 (MQ=255)

                                                                              cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAATGTATTAATGATCTGAGATGTTTATGCAAAACCGGGAAGAGaa                                               >  2:413788/1‑128 (MQ=255)

                                                                              cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               >  1:3682788/1‑128 (MQ=255)

                                                                              cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               >  2:3542649/1‑128 (MQ=255)

                                                                              cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               >  2:1812736/1‑128 (MQ=255)

                                                                                 aTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATg                                            >  2:2866692/1‑128 (MQ=255)

                                                                                      tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGTGAAGAGAAATGCTAGa                                       >  2:3187637/1‑128 (MQ=255)

                                                                                         aattaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAATTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaa                                    <  2:1993904/128‑1 (MQ=255)

                                                                                          attaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaaa                                   <  1:2980113/128‑1 (MQ=255)

                                                                                             aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  2:3744803/128‑1 (MQ=255)

                                                                                              atatAATTGAAACTAATCAAATAGATGTAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:149767/128‑1 (MQ=255)

                                                                                              ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  2:3466917/128‑1 (MQ=255)

                                                                                              ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  2:1384492/128‑1 (MQ=255)

                                                                                              ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  2:260201/128‑1 (MQ=255)

                                                                                              ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:2124276/128‑1 (MQ=255)

                                                                                               taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAttt                              >  2:3677520/1‑128 (MQ=255)

                                                                                                  aaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaa                           <  2:306512/128‑1 (MQ=255)

                                                                                                     tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaaa                        >  1:2616647/1‑128 (MQ=255)

                                                                                                      gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       >  2:2306990/1‑128 (MQ=255)

                                                                                                      gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       >  1:938770/1‑128 (MQ=255)

                                                                                                      gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       >  1:459277/1‑128 (MQ=255)

                                                                                                       aaaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:2734129/119‑1 (MQ=255)

                                                                                                        aaCAAATCAAATAGATGAAAAATAGTTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAatat                     >  2:663237/1‑128 (MQ=255)

                                                                                                        aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCTGAGATGTTTATGCATATCCGGGAAGAGAAATGCTAGAATAAAATTTAAAAAttac                     >  1:825655/1‑127 (MQ=255)

                                                                                                         aCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAtt                    >  1:3778501/1‑128 (MQ=255)

                                                                                                             atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  2:2726487/1‑128 (MQ=255)

                                                                                                             atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  1:3141644/1‑128 (MQ=255)

                                                                                                             atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  1:2007585/1‑128 (MQ=255)

                                                                                                                  atAGATGAAAAATATTATATAAAAAGTTTAAAAGATGAGTTTTGATTAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaa           <  2:1892045/128‑1 (MQ=255)

                                                                                                                        gAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaa     <  1:2924006/128‑1 (MQ=255)

                                                                                                                         aaaaaTTATTTATAAAAAGTTTAAATGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  2:3403312/128‑1 (MQ=255)

                                                                                                                         aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  1:1004299/128‑1 (MQ=255)

                                                                                                                           aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATa  <  2:3337422/128‑1 (MQ=255)

TATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281490‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`