New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 228871 =275 (0.960)5 (0.020) 5/440 24.2 1.8% coding (104/1425 nt) TMARI_RS01105 glycine dehydrogenase
?NC_021214 229168 = 292 (1.040)coding (401/1425 nt) TMARI_RS01105 glycine dehydrogenase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGCACAACATTTTTTTCAGTTCGTACATGAGCCGAAGACTGCCCTGAACAGTTTCGACGGGCTGATACGGGTGTATCTCTCTGAATCCTTCGAGATTCGCAGCTTTTTCGTTCAGTTTGGGATTGTACTTCATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCGGAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_021214/229113‑228871
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggagAACTCACGGGAATGCTGATAGTGAGAGAGTACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGCCCACGGTACCAACCCAGCATCTGCTTCGATGGTTGGTTTTGAGGTGGTGGAGATAAAGAGCAAAAACGGTATGGTGGATGTGGAAGATTTGAAAAAACTACTGGATGAAGAAGTAGCCGCTGTGATGCTCACAAATCCAAACAC  >  NC_021214/229168‑229396
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
CGCACAACATTTTTTTCAGTTCGTACATGAGCCGAAGACTGCCCTGAACAGTTTCGACGGGCTGATACGGGTGTATCTCTCTGAATCCTTCGAGATTCGCAGCTTTTTCGTTCAGTTTGGGATTGTACTTCATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCG                                                                                                                                                                                                                                          >  2:122705/1‑240
CGCACAACATTTTTTTCAGTTCGTACATGAGCCGAAGACTGCCCTGAACAGTTTCGACGGGCTGATACGGGTGTATCTCTCTGAATCCTTCGAGATTCGCAGCTTTTTCGTTCAGTTTGGGATTGTACTTCATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCG                                                                                                                                                                                                                                          >  2:655149/1‑240
 GCACAACATTTTTTTCAGTTCGTACATGAGCCGAAGACTGCCCTGAACAGTTTCGACGGGCTGATACGGGTGTATCTCTCTGAATCCTTCGAGATTCGCAGCTTTTTCGTTCAGTTTGGGATTGTACTTCATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGGGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCGG                                                                                                                                                                                                                                         >  2:769708/1‑240
                                                                                             GATTCGCAGCTTTTTCGTTCAGTTTGGGATTGTACTTCATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCGGAGAACTCACGGGAATGCTGATAGTGAGAGAGTACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGCCCAC                                                                                                                                              <  2:69005/239‑1
                                                                                                                                  CATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCGGAGAACTCACGGGAATGCTGATAGTGAGAGAGTACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGC                                                                                                                                                  >  1:75623/1‑198
                                                                                                                                  CATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCGGAGAACTCACGGGAATGCTGATAGTGAGAGAGTACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGC                                                                                                                                                  <  2:75623/198‑1
                                                                                                                                                                                                                                      ggagctaCCGGAGAACTCACGGGAATGCTGATAGTGAGAGAGTACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGCCCACGGTACCAACCCAGCATCTGCTTCGATGGTTGGTTTTGAGGTGGTGGAGATAAAGAGCAAAAACGGTATGGTGGATGTGGAAGATTTGAAAAAACTACTGGATGAAGAAGTAGCCGCTGTGATGCTCACAAATCCAAAC    <  1:1187577/233‑1
                                                                                                                                                                                                                                        agctaCCGGAGAACTGAAGGGAAGGCGGAAAGTGAGAGAGTACTTTAAAAACAGAGGAGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGCCCACGGTACCAACCCAGCATCTGCTTCGATGGTTGGTTTTGAGGTGGTGGAGATAAAGAGCAAAAACGGTATGGTGGATGTGGAAGATTTGAAAAAACTACTGGATGAAGAAGTAGCCGCTGTGATGCTCACAAAACCAAACAC  <  1:6669/235‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
CGCACAACATTTTTTTCAGTTCGTACATGAGCCGAAGACTGCCCTGAACAGTTTCGACGGGCTGATACGGGTGTATCTCTCTGAATCCTTCGAGATTCGCAGCTTTTTCGTTCAGTTTGGGATTGTACTTCATGGTGCAGGAACCGAGGGGGTAGATTCCGAGATCCACAGAGTAGTTCTTTCTGGCAAGATTGGTGTAATGCCTCACCAAATCAGGCTCGCTCACCTCGGGAAGCTCCGGAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_021214/229113‑228871
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggagAACTCACGGGAATGCTGATAGTGAGAGAGTACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGCCCACGGTACCAACCCAGCATCTGCTTCGATGGTTGGTTTTGAGGTGGTGGAGATAAAGAGCAAAAACGGTATGGTGGATGTGGAAGATTTGAAAAAACTACTGGATGAAGAAGTAGCCGCTGTGATGCTCACAAATCCAAACAC  >  NC_021214/229168‑229396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.