Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_021214449,0560TC47.4% ‑5.2 / 21.6 19G501G (GGA→GGGTMARI_RS02175methyl‑accepting chemotaxis protein 1
Reads supporting (aligned to +/- strand):  ref base T (6/4);  new base C (5/4);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCTTCCAAGGATCTCTCTGAATCTCTTTGTGACTTCTTCTCCCATCACCGCTATCTCGTCCACTCCTTCGAACATCTCTTTCGAGCCGTTTTCTACGTGTTCTATCGTTGTTCTCAGGCTGCTCAGCATCTTCGCTATGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTCTGACCTTTCTGTC  >  NC_021214/448840‑449290
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          
ccTTCCAAGGATCTCTCTGAATCTCTTTGTGACTTCTTCTCCCATCACCGCTATCTCGTCCACTCCTTCGAACATCTCTTTCGAGCCGTTTTCTACGTGTTCTATCGTTGTTCTCAGGCTGCTCAGCATCTTCGCTATGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCCGCTTCTATc                                                                                                                                                                                                                       >  1:979772/1‑238 (MQ=17)
                                         ccacccccGCTACTTCGTCCCCTCCTTCGAACAGCTCTTTCGAGCCGTTTTCTACGTTTTCTATCGTTGTTCTCAGGCTGCTCAGCTTCTTCGCTATGTCTTCTGCCGCCCTCTGGCTCCCTTCTGCGTGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCCCCAGCTCGAGCCGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAg                                                                                                                                                                             <  2:979772/233‑1 (MQ=11)
                                                                                         ttctatctgtttCTATCGTCGTCCACAAACTGCCCAGCTGCTTGCGTATGTTTCCTGCCGCCCTCGGACTTTCTTCTTCGAGTTTCCTTATCTCTTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCCGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCTGAGTCAACGAg                                                                                                                              <  2:1175561/228‑1 (MQ=11)
                                                                                                                             gCATCTTCGCTATGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGCGTCAACGAGTTCCCCCAGGTAGTCCCCCTGTCTTTCTGCTGACCCt                                                                                         >  2:838468/1‑239 (MQ=9)
                                                                                                                                 cTTCGCTATTCCTCCTGTCGCCCTCTGGCTCTCTTCTTCGAGTTTCCTTCTCTCGTCTGCCCCCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCTGAGTCAACGAGTTCTCTAAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGa                                                                                     <  2:29992/239‑1 (MQ=11)
                                                                                                                                   tCGCTATGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATAATCTTGGCTGAGTCAACGACTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTCAgt                                                                                   >  2:116682/1‑239 (MQ=9)
                                                                                                                                   tCGCTATGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCCGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGCGCTGATCGTGTCCACGATCTCTCCTTTCGTCTTGGCCGAGTCAACTAGGTCCCCCAGGGATTCCCTCGTTCCATTGTCTGACCCTTTGCgt                                                                                   >  2:373448/1‑239 (MQ=11)
                                                                                                                                        aTGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCTGAGTCAACGAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAgtttgtt                                                                              >  1:403799/1‑239 (MQ=14)
                                                                                                                                                                 cttctGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCTGAGTCAACGAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTAc                                                      >  2:280962/1‑238 (MQ=14)
                                                                                                                                                                      gCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCTGAGTCAACGAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTActctctc                                                <  1:280962/239‑1 (MQ=14)
                                                                                                                                                                             ttcTTATCTCTGCTCCCCCCACCTCAAAGCTCTTGCCTGCTTCTCCAGCGCGGACTGCTTCTACCGTCGCGTTCTACGCGAGACGGGTCGTCTGCTCTGCTATGGTGCTGATCGTGTCCAGTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGTTATTCCCTCTTTCTTTCTGCTGACCCTTTGAGTTTGTTTAGGATTCCTCCCACCTCTTCTCCTCTCTCTGTCCCt                                         <  2:1261475/237‑1 (MQ=11)
                                                                                                                                                                                                       aacccctttCCCGCCTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGTCCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTc               <  2:579425/232‑1 (MQ=9)
                                                                                                                                                                                                               tCCCGCCTCCCCAGCTCGGTCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTCTGACCttt       <  2:236914/239‑1 (MQ=9)
                                                                                                                                                                                                                     cTTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGa                                                                                             >  2:1279309/1‑148 (MQ=17)
                                                                                                                                                                                                                     cTTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGa                                                                                             <  1:1279309/148‑1 (MQ=17)
                                                                                                                                                                                                                     cTTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGGGTTTGTTTTTGACTCTTCCCACCCCTTTTACTTTCTCTGTCCCTTTTCTTTCCCCTTTCGTACCCCCTTCTGCCCTTTCTGTc  >  2:696834/1‑239 (MQ=11)
                                                                                                                                                                                                                     cTTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTCTGACCTTTCTGTc  >  2:1063024/1‑239 (MQ=14)
                                                                                                                                                                                                                     cTTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTCTGACCTTTCTGTc  >  2:57740/1‑239 (MQ=14)
                                                                                                                                                                                                                     cTTCCCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTCTGACCTTTCTGTc  >  1:849639/1‑239 (MQ=14)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          
CCTTCCAAGGATCTCTCTGAATCTCTTTGTGACTTCTTCTCCCATCACCGCTATCTCGTCCACTCCTTCGAACATCTCTTTCGAGCCGTTTTCTACGTGTTCTATCGTTGTTCTCAGGCTGCTCAGCATCTTCGCTATGTCTTCTGTCGCCCTCTGGCTCTCTTCTGCGAGTTTCCTTATCTCGTCTGCCACCACCGCAAAGCCTCTTCCCGCTTCTCCAGCTCGAGCTGCTTCTATCGCTGCGTTCAACGCGAGCAGGTTCGTCTGCTCTGCTATCGAGCTGATCGTGTCCACTATCTCTCCTATCGTCTTGGCCGAGTCAACTAGTTCTCTCAGGTAGTCCCTCTGTCTTTCTGCTGACCCTTTGAGTTTGTTTATGACTCCTCCCACCGCTTCTACTCTCTCTGTCCCTTCTCTTGCCGCTTTCGTCACCGCTTCTGACCTTTCTGTC  >  NC_021214/448840‑449290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: