BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_021214	253,934	G→A	D165N (GAT→AAT)	TMARI_RS01225 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_021214	253,934	0	G	A	99.4%	1088.2 / ‑4.9	349	D165N (GAT→AAT)	TMARI_RS01225	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (167/180); total (167/182)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

TGCCATGTTAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAGATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACAGGGCTATAGACTTCACTTTGAGCAACATGGTGAATTCCGGCAT  >  NC_021214/253699‑254169

tgCCATGTTAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGCTTCAAACACCAATTTCCTTGTAAAtt                                                                                                                                                                                                                                          >  2:1382775/1‑239 (MQ=255)

 gCCATGTTAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATCTCCGGATTCTAACAGCACTTTCATTGTAAAttt                                                                                                                                                                                                                                         >  2:235372/1‑239 (MQ=255)

 gCCATGTTAGAACTCTTTGCCAGACGTTAGAGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTCGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAAttt                                                                                                                                                                                                                                         <  2:731913/239‑1 (MQ=255)

   cATGTTAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTAGTGAGGGAATATCCGGATTCTAACACCAATTTAATTGTAAAttt                                                                                                                                                                                                                                         >  2:911057/1‑237 (MQ=255)

     tGTTAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGGGAGAGTTTATCTTGTCGTGAGGGAATATCCGGGTTCTAACACCAATTTCATTGTAAATTTACTg                                                                                                                                                                                                                                     >  2:1077129/1‑239 (MQ=255)

       ttAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAATTTTACTGaa                                                                                                                                                                                                                                   >  2:581971/1‑239 (MQ=255)

          gAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGaaaaa                                                                                                                                                                                                                                >  1:444055/1‑239 (MQ=255)

           aaCTCTTTTCCAGACGTTATGGAGTAAGGCTCGCAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTAATTGTAAATTTACTGaaaaa                                                                                                                                                                                                                                >  1:953017/1‑238 (MQ=255)

               ctTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATTGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACtt                                                                                                                                                                                                                            >  1:781416/1‑238 (MQ=255)

               ctTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACtt                                                                                                                                                                                                                            >  2:300361/1‑238 (MQ=255)

               ctTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACtt                                                                                                                                                                                                                            >  2:656948/1‑238 (MQ=255)

                ttttCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTc                                                                                                                                                                                                                          >  2:285539/1‑239 (MQ=255)

                 tttCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCg                                                                                                                                                                                                                         >  1:1155382/1‑239 (MQ=255)

                   tCCAGACGTTATGGAGTAAGGCTCGACGACATGTTCGATCTGTCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGAATTTCTGATCGTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  2:521130/239‑1 (MQ=255)

                   tCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  1:154985/239‑1 (MQ=255)

                   tCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  1:295410/239‑1 (MQ=255)

                   tCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       >  1:627093/1‑239 (MQ=255)

                   tCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  2:119954/239‑1 (MQ=255)

                   tCCAGAAGTGATTGAGTAAGGGTCGAGGAAATGTTCGATCTGCCAGACGTCGAACCAGAAGAGCTCGATTTTACTACTGGTGAGTTTCTGATCGTCCCCGACATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  2:1245469/239‑1 (MQ=255)

                          gTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  1:927208/232‑1 (MQ=255)

                          gTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGtttt                                                                                                                                                                                                                >  2:1069390/1‑239 (MQ=255)

                          gTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGtttt                                                                                                                                                                                                                >  2:829223/1‑239 (MQ=255)

                          gTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGtttt                                                                                                                                                                                                                >  1:884930/1‑239 (MQ=255)

                            tATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTtat                                                                                                                                                                                                              >  2:673582/1‑239 (MQ=255)

                            tATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCAAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTta                                                                                                                                                                                                               <  2:163147/238‑1 (MQ=255)

                              tGGAGTAATGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATag                                                                                                                                                                                                            <  1:572470/239‑1 (MQ=255)

                                 aaaaaGTCTCAAAGAAATGTTCGCGCTGCCAGACTTCGAAACAGAAGAACTCGATTTTCCTTCTGGTGATGTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGGTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATagaag                                                                                                                                                                                                         <  1:1191788/236‑1 (MQ=255)

                                   tAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGACGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAgaagaa                                                                                                                                                                                                       >  2:1264647/1‑239 (MQ=255)

                                   tAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATag                                                                                                                                                                                                            <  1:223603/234‑1 (MQ=255)

                                   tAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAgaagaa                                                                                                                                                                                                       >  1:1394901/1‑239 (MQ=255)

                                   tAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAgaagaa                                                                                                                                                                                                       >  2:1133508/1‑239 (MQ=255)

                                     aGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGaaaa                                                                                                                                                                                                     >  1:866120/1‑239 (MQ=255)

                                     aGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGaaaa                                                                                                                                                                                                     >  1:12152/1‑239 (MQ=255)

                                      ggCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAg                                                                                                                                                                                                    >  1:208021/1‑239 (MQ=255)

                                        cTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGt                                                                                                                                                                                                   <  2:934657/238‑1 (MQ=255)

                                        cTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTg                                                                                                                                                                                                  >  2:509155/1‑239 (MQ=255)

                                            aagaaATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAgg                                                                                                                                                                                              >  1:130833/1‑239 (MQ=255)

                                                 aTGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTGCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCGGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAAGTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATa                                                                                                                                                                                         <  1:360640/239‑1 (MQ=255)

                                                   gTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGa                                                                                                                                                                                       <  1:1204705/239‑1 (MQ=255)

                                                      cGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTTATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATGTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAAtt                                                                                                                                                                                    <  1:749451/239‑1 (MQ=255)

                                                         tCTGCCAGACTTCGAACCAGAAAAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGGGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGACAAAACTTTCGGACGGTTTTATAGAAGAAAAGAGGCGGTGCTAGACTTcat                                                                                                                                                                                 >  2:1219521/1‑237 (MQ=255)

                                                          cTGCCAGACTTCGAACCAGAATAACTCGATTTTCCTTCTGGTGATTTTCTTATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCttt                                                                                                                                                                                <  2:204108/239‑1 (MQ=255)

                                                              cAGTCTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTg                                                                                                                                                                            <  2:969322/239‑1 (MQ=255)

                                                                   ttCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACga                                                                                                                                                                       <  2:1037971/239‑1 (MQ=255)

                                                                   ttCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACga                                                                                                                                                                       <  1:484369/239‑1 (MQ=255)

                                                                   ttCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACga                                                                                                                                                                       <  1:1133508/239‑1 (MQ=255)

                                                                   ttCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACga                                                                                                                                                                       <  2:368439/239‑1 (MQ=255)

                                                                     cGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACgaag                                                                                                                                                                     >  1:980799/1‑239 (MQ=255)

                                                                        aCCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACgaaggaa                                                                                                                                                                  <  2:26849/239‑1 (MQ=255)

                                                                          cAGAAGAACTCGATTTTCCTTCTTGTGTTTTTTTGATCTTCCCAGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAaga                                                                                                                                                                <  2:566087/239‑1 (MQ=255)

                                                                             aagaaCTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAagagag                                                                                                                                                             >  1:650220/1‑239 (MQ=255)

                                                                                  cTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTCGGAGAGAGTGTTTATCTTGTCGTGAGTGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGACAAGTGGAGGTGAAAGAATTATTTAGTGAACGAAGGAAACGAGGtgtg                                                                                                                                                        >  2:1106123/1‑239 (MQ=255)

                                                                                        tttCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAAt                                                                                                                                                  <  2:444055/239‑1 (MQ=255)

                                                                                                 ggTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  2:17631/161‑1 (MQ=255)

                                                                                                  gTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCtt                                                                                                                                        >  1:164364/1‑239 (MQ=255)

                                                                                                      ttttCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGga                                                                                                                                     <  2:627093/238‑1 (MQ=255)

                                                                                                            gATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGGGAAGCTTTGGAGCGAGAGTTTATCTTGTCGTGAGGGGATATCAGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGTAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGATGGCAGAGAGGTGTGAAGAATGAGGGGGCCTGgattgatta                                                                                                                              >  2:595521/1‑238 (MQ=255)

                                                                                                                 tCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAAtt                                                                                                                                                                                    >  1:553025/1‑180 (MQ=255)

                                                                                                                 tCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGACAAGATTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACGCCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAAGGAGGGTGCTTGGATTGATTCTTGCt                                                                                                                         <  2:1159234/239‑1 (MQ=255)

                                                                                                                 tCCCCGAAATGATCGGTCACGGTCGCAGGGTGATGGGATTAATGTCTGCCATGAGAAGTTTTGGATAGAGATTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCt                                                                                                                         <  2:79574/239‑1 (MQ=255)

                                                                                                                            aTCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAg                                                                                                              >  1:1239350/1‑239 (MQ=255)

                                                                                                                             tCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATGTTGTCGTGAGGGAATATCCGGAGTCTAACACCAATTTCATCGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGCAGAGAGGTGGGCAGACTGCGGGTGCTTGGCTTGATTCTTGCTGGGGGAAAAAGt                                                                                                             >  2:1299149/1‑239 (MQ=255)

                                                                                                                              cGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  2:1212370/132‑1 (MQ=255)

                                                                                                                                    cGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       >  1:1065285/1‑126 (MQ=255)

                                                                                                                                     gTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAAAGAAGGAAGAGAGGAGTGGAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGGAACAGAGATAGttt                                                                                                     >  2:860637/1‑239 (MQ=255)

                                                                                                                                       tctcAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGGCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTtgt                                                                                                   <  2:717322/239‑1 (MQ=255)

                                                                                                                                        ctcAGGGTGATGGGATTCATGTCTGCCGTGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTtgtg                                                                                                  <  2:884930/239‑1 (MQ=255)

                                                                                                                                              gTGATTGGATTAATTTCTGCCATGGGAATCTTTGGATAGAGAATTTATCTTATAGTGAGGGAATATCCGGATTCTAATATCAATTTCATTGTAAATTTACTGAAAATACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTATAATGAGCGAAGGAAGAGAGGTGTTAAGAATGATGATCCTCGGATTGACTCTTGCTGGTGGAAAAAGTGATCGTTTgggggc                                                                                               <  2:225054/236‑3 (MQ=255)

                                                                                                                                                 aTGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGAt                                                                                                          >  1:713967/1‑222 (MQ=255)

                                                                                                                                                        tCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTg                                                                                  >  1:966633/1‑239 (MQ=255)

                                                                                                                                                               tGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGAt                                                                                                          <  1:430675/208‑1 (MQ=255)

                                                                                                                                                               tGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGagag                                                                              >  1:40728/1‑236 (MQ=255)

                                                                                                                                                               tGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGGGATCGTTTGTGGCCTCTGACGAAGGTGGAAGCCa                                                                           >  2:461374/1‑239 (MQ=255)

                                                                                                                                                                 ccATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGAGCTTGGATTGATTTTTGCTGGGGGAAAAAGGGATGGGTTGTGGCCTTGGACGAAGGGGAGAGGCAGt                                                                         >  2:19371/1‑239 (MQ=255)

                                                                                                                                                                    tGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGTAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTgcg                                                                      >  2:418642/1‑239 (MQ=255)

                                                                                                                                                                    tGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTgcg                                                                      >  1:846129/1‑239 (MQ=255)

                                                                                                                                                                      agaAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGGGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTgcggc                                                                    >  1:528737/1‑239 (MQ=255)

                                                                                                                                                                           cTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGAGGAGGTGATAGAATTCTTTAGTGAACGATGGAAGAGAGGGGTGAAGAATGAGGGTGATTGGCTTGATTCTTGCTGGGGGACAAAGTGACGGTTGGTGGCTTTTGACGAAGGTGGGTCCCAGTGcggcggat                                                                >  2:1337527/1‑236 (MQ=255)

                                                                                                                                                                            tttGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAGAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTcc                                                              <  1:101176/239‑1 (MQ=255)

                                                                                                                                                                                  gagagagTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTgtgt                                                         <  1:267825/238‑1 (MQ=255)

                                                                                                                                                                                  gagaaaaTTTATCTTGGCGTGAGGGAATATCCGGATTCTAACCCCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTgtgt                                                         <  1:1077129/238‑1 (MQ=255)

                                                                                                                                                                                     agagTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGt                                                                                                                                                                                                   >  1:301311/1‑97 (MQ=255)

                                                                                                                                                                                     agagTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGCGGAGGTAATAGAATTCTTTCGTGAACGCAGGATGAGAGGTGAGAAGAAGGAGGGAGCTTGTATTGATTATAGCTGGGGGAATAAGTGATCGTTTGTGGCCACTGATGAAGGTGTGCGCCAGGGCGGTGATTctagttgttgt                                                     >  2:1058908/1‑229 (MQ=255)

                                                                                                                                                                                      gagTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTg                                                      <  1:184642/237‑1 (MQ=255)

                                                                                                                                                                                        gTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGaa                                                   <  1:693693/238‑1 (MQ=255)

                                                                                                                                                                                        gTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGa                                                    <  2:1370928/237‑1 (MQ=255)

                                                                                                                                                                                        gTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGa                                                    <  1:465831/237‑1 (MQ=255)

                                                                                                                                                                                           tATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTa                                               <  1:237049/239‑1 (MQ=255)

                                                                                                                                                                                           tATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTa                                               <  1:911057/239‑1 (MQ=255)

                                                                                                                                                                                           tATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTa                                               <  1:582913/239‑1 (MQ=255)

                                                                                                                                                                                             tCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAGATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACa                                             <  1:767705/239‑1 (MQ=255)

                                                                                                                                                                                             tCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACa                                             <  1:1261979/239‑1 (MQ=255)

                                                                                                                                                                                             tCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCAGCGGTTCCTGTGTTTGGAAAGTACa                                             <  1:659949/239‑1 (MQ=255)

                                                                                                                                                                                                       aGGGAATATCCGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACAGGGCTATAGa                                   >  1:791270/1‑239 (MQ=255)

                                                                                                                                                                                                                ccGGATTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACAGGGCTATAGACTTCACttt                          <  1:133733/239‑1 (MQ=255)

                                                                                                                                                                                                                    aTTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGa                                                                                                                                                                                                                       <  1:797646/46‑1 (MQ=255)

                                                                                                                                                                                                                    aTTCTAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACAGGGCTATAGACTTCACTTTGAg                       <  2:255695/238‑1 (MQ=255)

                                                                                                                                                                                                                        tAACACCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACga                                                                                                                                                                       <  1:137684/90‑1 (MQ=255)

                                                                                                                                                                                                                            acCAATTTCATTGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACAGGGCTATAGACTTCACTTTGAGCAACATGGt              <  2:77944/239‑1 (MQ=255)

                                                                                                                                                                                                                                       tGTAAATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTTGAAAGTACCGGGCTATAGACTTTACTTTTAGCAAAAAGGGGAATTTCGGCa   >  1:722753/1‑239 (MQ=255)

TGCCATGTTAGAACTCTTTTCCAGACGTTATGGAGTAAGGCTCGAAGAAATGTTCGATCTGCCAGACTTCGAACCAGAAGAACTCGATTTTCCTTCTGGTGATTTTCTGATCTTCCCCGAAATGATCGGTCACGTTCTCAGGGTGATGGGATTCATGTCTGCCATGAGAAGCTTTGGAGAGAGAGTTTATCTTGTCGTGAGGGAATATCCGGATTCTAACACCAATTTCATTGTAGATTTACTGAAAAAACTTTCGGACGGTTTTATAGAAGAAAAGTGGAGGTGATAGAATTCTTTAGTGAACGAAGGAAGAGAGGTGTGAAGAATGAGGGTGCTTGGATTGATTCTTGCTGGGGGAAAAAGTGATCGTTTGTGGCCTCTGACGAAGGTGAGAGCCAGTGCGGCGGTTCCTGTGTTTGGAAAGTACAGGGCTATAGACTTCACTTTGAGCAACATGGTGAATTCCGGCAT  >  NC_021214/253699‑254169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`