New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 = 453135405 (1.440)6 (0.020) 6/392 12.4 1.6% coding (496/1272 nt) TMARI_RS02190 carbohydrate ABC transporter substrate‑binding protein
?NC_021214 = 453205 338 (1.250)coding (426/1272 nt) TMARI_RS02190 carbohydrate ABC transporter substrate‑binding protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAATAGATTAACAAAATCCTCAAGAGCTTTGATTGCTTTTGGATCCTTATCGAGAACAAGATTCAGATTCTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_021214/452926‑453135
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtccagggTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAGGA  <  NC_021214/453205‑452969
                                                                                                                                                                                                                                                                                                                                                                                                                                                               
GAATAGATTAACAAAATCCTCAAGAGCTTTGATTGCTTTTGGATCCTTATCGAGAACAAGATTCAGATTCTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGGTCTATGGGTCAATGTGGATTACTTCTAAAA                                                                                                                                                                                                                 >  2:1060650/1‑240
                                 TGCTTTTGGATCCTTATCGAGAACAAGATTCAGATTCTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAG                                                                                                                                                                                <  1:1060650/240‑1
                                            CCTTATCGAGAACAAGATTCAGATTCTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGG                                                                                                                                                                                                                                                >  1:1011885/1‑165
                                            CCTTATCGAGAACAAGATTCAGATTCTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGG                                                                                                                                                                                                                                                <  2:1011885/165‑1
                                                                     CTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGT                                                                                                                                                                                                          <  2:325019/178‑1
                                                                     CTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGT                                                                                                                                                                                                          >  1:325019/1‑178
                                                                                                                                             AGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGG                                                                                                                                                                                                           <  1:1002365/105‑1
                                                                                                                                             AGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGG                                                                                                                                                                                                           >  2:1002365/1‑105
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTACCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGAT       >  1:457448/1‑240
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGAGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCAAAAGTGCGGGATGAGAATGTGAATCTTGTTCTCGAT       >  2:1228582/1‑240
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGA                                                                                                                                     >  2:998896/1‑114
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGA                                                                                                                                     >  2:262585/1‑114
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGA                                                                                                                                     <  1:998896/114‑1
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGA                                                                                                                                     <  1:262585/114‑1
                                                                                                                                                                                                          GTCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGATGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATAAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGAT       >  2:806657/1‑240
                                                                                                                                                                                                           TCCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATA      >  2:1265665/1‑240
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAA     >  2:931831/1‑240
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATA      <  2:532204/239‑1
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATA      <  1:386357/239‑1
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTG                                                                                 >  1:90792/1‑164
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTG                                                                                 <  2:90792/164‑1
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCAT                                                                                                         >  2:1143894/1‑140
                                                                                                                                                                                                            CCAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCAT                                                                                                         <  1:1143894/140‑1
                                                                                                                                                                                                             CAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAG    <  1:436385/240‑1
                                                                                                                                                                                                             CAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAG    <  1:491248/240‑1
                                                                                                                                                                                                             CAGGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAG    <  1:806657/240‑1
                                                                                                                                                                                                               GGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTC            >  2:755812/1‑230
                                                                                                                                                                                                               GGGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTC            <  1:755812/230‑1
                                                                                                                                                                                                                GGTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAGGA  >  1:361786/1‑239
                                                                                                                                                                                                                 GTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAGG   <  1:187302/237‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                               
GAATAGATTAACAAAATCCTCAAGAGCTTTGATTGCTTTTGGATCCTTATCGAGAACAAGATTCAGATTCTCATCCAGCACTTTGGCTCCTCTGATTGCAAGATAGTTCGCTATTCTTTCAGCAGAATACTGCATGGACATAGCGTATGGCAGGTTATTTGCCTCTTTCACCTTTCTCAAAACTTCCACGAACTCTTCCCATGTCCAGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_021214/452926‑453135
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtccagggTCTATGGGTCAATGTGGATTACTTCGAAAAAGCTGGTATACCTTTCCCACCACTTGGTGGAAGAGAAGAGCCCTGGACATGGGAAGAGTTCGTGGAAGTTTTGAGAAAGGTGAAAGAGGCAAATAACCTGCCATACGCTATGTCCATGCAGTATTCTGCTGAAAGAATAGCGAACTATCTTGCAATCAGAGGAGCCAAAGTGCTGGATGAGAATCTGAATCTTGTTCTCGATAAGGA  <  NC_021214/453205‑452969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.