New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_021214	848026 =	237 (0.840)	6 (0.020)	6/400	12.5	2.7%	coding (605/2367 nt)	TMARI_RS04210	phenylalanine‑‑tRNA ligase subunit beta
	?	NC_021214	848219 =	208 (0.760)					coding (798/2367 nt)	TMARI_RS04210	phenylalanine‑‑tRNA ligase subunit beta
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACCCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_021214/848268‑848026 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgaCCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCA  >  NC_021214/848219‑848456                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   CACCCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACAT                                                                                                                                                                                                                                                   <  1:1254026/240‑1  ACCCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATC                                                                                                                                                                                                                                                  >  2:773987/1‑240  ACCCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATC                                                                                                                                                                                                                                                  >  1:943193/1‑240    CCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCGGGTCGAACGCATGAACGGGATGTCCCGGCTCGATCATCACGTAGTTCGGGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA                                                                                                                                                                                                                                                <  1:281591/240‑1    CCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA                                                                                                                                                                                                                                                >  2:1317734/1‑240    CCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA                                                                                                                                                                                                                                                >  1:563132/1‑240      TTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA                                                                                                                                                                                                                                                >  2:417418/1‑238      TTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA                                                                                                                                                                                                                                                >  1:868995/1‑238       TTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA                                                                                                                                                                                                                                                >  1:1176581/1‑237                                                           ATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGAACTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAA                                                                                                                                                                                         >  1:706240/1‑240                                                                          CAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTC                                                                                                                                                                          >  2:1062246/1‑240                                                                          CAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTC                                                                                                                                                                          <  1:1062246/240‑1                                                                            GCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGTGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGA                                                                                                                                                                        >  1:1270314/1‑240                                                                                                                                                                         CTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATC                                                                                                                                                                                                                                                  >  2:1280514/1‑73                                                                                                                                                                         CTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATC                                                                                                                                                                                                                                                  <  1:1280514/73‑1                                                                                                                                                                                                                                  CTTCTATCTCCACATCGAACTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGT                                                                                                                       >  1:292478/1‑139                                                                                                                                                                                                                                  CTTCTATCTCCACATCGAACTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGT                                                                                                                       <  2:292478/139‑1                                                                                                                                                                                                                                                TCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGG    >  2:324119/1‑240                                                                                                                                                                                                                                                TCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAG     >  1:488016/1‑239                                                                                                                                                                                                                                                  GACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCA  <  1:324119/240‑1                                                                                                                                                                                                                                                   ACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTG                   >  1:751496/1‑222                                                                                                                                                                                                                                                   ACCTGAACAGGCTCAAAAATAAAAGAATCGTTGGGAAATCTGCAAAGGGTGGGGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTG                   <  2:751496/222‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   CACCCTTTGCAGATTTCACAACGATTCTTTTATTTTTGAGCCTGTTCAGGTCGAACGCATGAACGGGATGTCCCAGCTCGATCATCACGTAGTTCGTGGCGTCGACCACGTTGTTCAGAGAACGAACCCCTGCCGCTACGAGCCTCGCCTTCATCCAGAGCGGTGAATCTTTCACGGTAACACCTTTCATTATTCGAGCAGAGTATCTGGGACAACCCTCCACGTCTTCTATCTCCACATCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_021214/848268‑848026 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgaCCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCA  >  NC_021214/848219‑848456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.