Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,726,6510TG62.5% ‑6.5 / 15.3 24noncoding (2534/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (5/4);  new base G (0/15);  total (5/19)
Fisher's exact test for biased strand distribution p-value = 2.96e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCG  >  NC_000913/2726540‑2726765
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cccctttCAATTCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGAc                                                                                       <  1:1962240/135‑1 (MQ=1)
    tctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCg                                                                                   >  1:637278/8‑141 (MQ=1)
     ctcaactctcTAACGTCCACGGAATATAGGGACCTAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGTCCTTCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  2:209286/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  2:890118/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  2:53590/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  1:2341400/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  2:480272/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  2:1877066/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  1:547010/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  1:548015/135‑1 (MQ=1)
     ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                                                  <  2:1726903/135‑1 (MQ=1)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:1977641/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  2:917543/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:1895883/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:517459/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:258604/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:2523114/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:1977741/138‑1 (MQ=0)
       caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                                                <  1:1977583/138‑1 (MQ=0)
                               cagcgACCGAACGGACACACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGa                                                        <  1:2127253/137‑1 (MQ=0)
                                                 actacgTTCTAAACCCAGCTCGCGTACCACTTTAAAAGTATAACAGACATACCCTTGGGACCTACTTCAGCCCCAGTAAGTGATTAGCCGACATCGAGGTCCCAAACACCGCCTTCGAAATGAAATCGTGGGAGGTATCAg                                      >  2:2219750/4‑141 (MQ=1)
                                                                catcTCGCGAACCACATTAAATTTATAAAAGCCATACCCTTTGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGa                       >  1:946665/4‑141 (MQ=1)
                                                                  gcacgcgTACCACTAAAAATGGCGAACAGCCATACCCTAGGGACCTACTTCAGCCCCAGGATGTGATGATCCGACATCTACGAGCCAAACACCGCCGTCTAAATGAACTCTTGGGCGGTATCAGCATGTTATCCCCGGAGt                     >  2:1655482/4‑141 (MQ=1)
                                                                                     tgtcGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTTGGCGGTATCAGCCTTATATCCCCGGAGTACCTTTAATCCTTTGAGct  >  2:528286/4‑140 (MQ=1)
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CCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCG  >  NC_000913/2726540‑2726765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: