Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0350GT41.7% ‑6.4 / 11.5 12noncoding (2150/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (3/2);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGC  >  NC_000913/2726918‑2727161
                                                                                                                      |                                                                                                                              
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                         >  1:285495/4‑141 (MQ=1)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                         >  1:372530/4‑141 (MQ=1)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGcc                                                                                                         >  1:1676223/4‑141 (MQ=1)
               cccacacAAACTACCCACCACACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTac                                                                                          <  1:1655041/135‑1 (MQ=0)
                        acgaCCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCCTCCCACCTATCCTACACATCAAgg                                                                                 <  1:1384143/138‑1 (MQ=1)
                                  aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                       <  1:601444/138‑1 (MQ=0)
                                       cgggccccAACCCGGAATACGGGTCAACGATACAACACCAAACACTCAAGGCTGGAATTTCAACGTCGCCTCCCTGCAGACTGGCGTCCTCAATACAAAGCCACCAACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                  <  2:47128/134‑1 (MQ=0)
                                                     gataaggggTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTaaa                                                    >  1:2429157/7‑141 (MQ=0)
                                                                                 cagtAAAGCGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACAATTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcgc                        >  2:1134417/4‑141 (MQ=0)
                                                                                     tacaGGGTGGTATTTCAAGGTCGGCTCCATGCAGACGGGCGTCCACACTTCACAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCggg                     <  2:291690/138‑1 (MQ=0)
                                                                                                       ggacGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACACTGCATCTTCACAg   <  1:2671229/138‑1 (MQ=1)
                                                                                                        atcgccaccATGCAGACTGGCGTCCACAATTCAAACCCACACAACAATCCAACACATCAACGCACAAAGATAAGAGTCAAGCAATATAAAACGATCACGGCGTCAATCCGACTTGCCGCGGTTACACTGCAACTTCACAGc  >  2:2498660/8‑141 (MQ=0)
                                                                                                                      |                                                                                                                              
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGC  >  NC_000913/2726918‑2727161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: