Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0460AT46.2% ‑6.7 / 14.1 13noncoding (2139/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base T (3/3);  total (7/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGT  >  NC_000913/2726918‑2727180
                                                                                                                                 |                                                                                                                                      
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                                            >  1:285495/4‑141 (MQ=1)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                                            >  1:372530/4‑141 (MQ=1)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGcc                                                                                                                            >  1:1676223/4‑141 (MQ=1)
               cccacacAAACTACCCACCACACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTac                                                                                                             <  1:1655041/135‑1 (MQ=0)
                        acgaCCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCCTCCCACCTATCCTACACATCAAgg                                                                                                    <  1:1384143/138‑1 (MQ=1)
                                  aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                                          <  1:601444/138‑1 (MQ=0)
                                       cgggccccAACCCGGAATACGGGTCAACGATACAACACCAAACACTCAAGGCTGGAATTTCAACGTCGCCTCCCTGCAGACTGGCGTCCTCAATACAAAGCCACCAACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                     <  2:47128/134‑1 (MQ=0)
                                                     gataaggggTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTaaa                                                                       >  1:2429157/7‑141 (MQ=0)
                                                                                 cagtAAAGCGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACAATTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcgc                                           >  2:1134417/4‑141 (MQ=0)
                                                                                     tacaGGGTGGTATTTCAAGGTCGGCTCCATGCAGACGGGCGTCCACACTTCACAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCggg                                        <  2:291690/138‑1 (MQ=0)
                                                                                                       ggacGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACACTGCATCTTCACAg                      <  1:2671229/138‑1 (MQ=1)
                                                                                                        atcgccaccATGCAGACTGGCGTCCACAATTCAAACCCACACAACAATCCAACACATCAACGCACAAAGATAAGAGTCAAGCAATATAAAACGATCACGGCGTCAATCCGACTTGCCGCGGTTACACTGCAACTTCACAGc                     >  2:2498660/8‑141 (MQ=0)
                                                                                                                           gcttCCACACTTCAAAGCCTCCCACCTATCCAAAACATCAAGGCTCAAAGTTCAGTGTCAAGCTTTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGCGTACTCTGCATCTTCACAGCGAGATCAATATCACTGAGt  >  2:2729688/4‑141 (MQ=1)
                                                                                                                                 |                                                                                                                                      
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGT  >  NC_000913/2726918‑2727180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: