| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,412,781 | 0 | C | G | 35.4% | 86.7 / 12.4 | 52 | S132C (TCT→TGT) | yjfM | DUF1190 domain‑containing protein YjfM |
| Reads supporting (aligned to +/- strand): ref base C (4/27); new base G (17/0); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCT > NC_000913/4412642‑4412914 | aTTGGCGCGCGGGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGTACTGTCAGTCTAAGTACGCAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCt < 2:1271679/141‑1 (MQ=255) cgcgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑tt < 1:1045212/141‑1 (MQ=255) cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑tttttt > 1:152400/1‑140 (MQ=255) acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGGGTGA‑TTTTTTTTTATCCGGGttt > 1:595474/1‑140 (MQ=255) acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGT‑TGTTTTTTTATCCggggtt > 1:786310/1‑138 (MQ=255) acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGG‑TTTTTTTTTATCCGGGTTa > 1:255967/1‑141 (MQ=255) acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGA‑TTTTTGTTAAGCCggggtt > 1:786202/1‑138 (MQ=255) cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTAt < 2:765131/141‑1 (MQ=255) aTGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGT‑TTTTTGTTTATCCGGGTTATTAGTAAAGATCGCGATg > 2:1689771/1‑141 (MQ=255) tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt < 2:1233718/141‑1 (MQ=255) tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt < 2:983163/141‑1 (MQ=255) tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt < 2:177252/141‑1 (MQ=255) tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt < 2:1233725/141‑1 (MQ=255) tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt < 1:295288/141‑1 (MQ=255) tGTTCCGAAGAATAGGACTCAGCAGAACTGTCAGTCTAAGTACGTAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGAGGAGCC‑gtt < 2:1135617/141‑1 (MQ=255) tGTTCCGAACAATATGACTCAGCAGAACTGTCAGTCTAAGTACGTAAATAGCTACTACGCCAATGTTGAACAGAGATGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGCGTTATTCGTAAAGATCGCGATGAGCC‑gtt < 2:1135611/141‑1 (MQ=255) agaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGA‑ATTTTTGTAATCCGGGTTAATCGTAAATATCGCGATGAGCC‑GTTTGGTtata > 2:386867/1‑141 (MQ=255) atatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTTTTTATGCCGGG‑TGTTTGTAAATATCGCGATTAGCCGGTGTGTTTaaaaa > 1:893427/1‑140 (MQ=255) atatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGT‑TTTTTTTTAATCCGGGGTTTTCGTTAAGATAGCGATGAGCC‑GTTTGTTTTTaaaa > 1:816866/1‑139 (MQ=255) atatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTTTTTAGCCCGGGTATTAGTAAAAATCGCGGTTAGCC‑GGTTTTTTTTaaaa > 1:893426/1‑139 (MQ=255) tGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAACGATCGCGATGAGCC‑GTTTGTTTATAACAgcg < 2:352807/141‑1 (MQ=255) tGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACGACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAgcg < 2:352610/141‑1 (MQ=255) gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGA‑TTTTTGTTAATCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAgcgg > 2:1579350/1‑141 (MQ=255) cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGt < 1:650381/141‑1 (MQ=255) cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGG‑TTTTTTTTTAGGCGGGGTTTTAGGAAAGATCGCGGTGATCC‑GGTTTTTTTTTAAAGcggcggg > 1:1368854/1‑140 (MQ=255) cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGA‑TTTTTTTTAATCCGGGTTATTAGTAAAGATCGCGAAGATCC‑GGTTGTTTTTAAAAGcggcggg > 1:1922392/1‑140 (MQ=255) gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGGGGT‑TTTTTTTTAATCCGGGGTTTTCGTAAAGATCGCGATGATCCGGTTTGTTT‑TAAAAGCGGCGGGTTCTAttttt > 1:1393071/1‑140 (MQ=255) cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATAGCGATGAGCC‑GTTTGTTTATAAAAGCGGCGGTTCCTCTTTTTCt > 2:1592361/1‑141 (MQ=255) cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGT‑TTTTTGTTAATCCGGGTTATTCGTTAATATAGCGATGAGCC‑GGTTGTTTATAACAGCGGCGGTTACTCtttttgt > 2:1592430/1‑138 (MQ=255) gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTc < 1:1796721/141‑1 (MQ=255) gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTc < 1:571012/141‑1 (MQ=255) tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTTATCCGGGTTATTAGTAAAGAACGCGATGTGCC‑GGTTGTTTTTTACAGCGGCGGTTACTCTGTTTCttgg > 2:577047/1‑139 (MQ=255) aaGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCgcgc < 2:430375/141‑1 (MQ=255) gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGccc < 2:1193415/141‑1 (MQ=255) gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTAATCCGGGGTATTGGTAAAGATAGCGATTAGCC‑GTTTGTTTATAACATCGGCGGTTGCTCTTTTTGTTAGCGccc > 2:713841/1‑141 (MQ=255) gAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGA‑TTTTTTTTTAGCCCGGGTAATAGTAAAAATCGCCAAGAAAC‑GTTTTTTTATAAAAGCGGCGGGTACTATTTTTCTTAGCTCCCAgac > 2:326923/1‑139 (MQ=255) aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCt < 2:1712780/141‑1 (MQ=255) gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgca < 1:1633283/141‑1 (MQ=255) gACAATGTTGAACAGAGTTGGATCCCGGACGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt < 1:484369/141‑1 (MQ=255) cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg < 2:1355922/141‑1 (MQ=255) cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg < 2:1698407/141‑1 (MQ=255) cAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGA‑TTTTTGTTAAACCGGGGTATTCGGAAATAACGCGAATAGCC‑GGTTGGTTATAAAAACGGCCGTTGCGCAGTTGGATAGCGCCCCGGCAGGCGCAGCACATCTgg > 2:1325756/1‑141 (MQ=255) tGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCCGGGTATTCGTAAAGATCGCGATTAGCC‑CTTTGTTTATAACAGCGGCGGTTACTCTTTTTCTTCGCGCCCAGTCTGGCGCCGCACATCTGGTGATTa > 2:1181980/1‑141 (MQ=255) tGAACAGAGTTGGATCCCGGTCGTTTATGGA‑TTATTGTTAAGCCGGGTTATTCATAAATATCCCGTTGATCC‑GTTTTTTTATAACACCGGCGGTTCCAAATTTTCGTAGCCCCCAGTCTGGCGCCTCACTTCTGGTGATTa > 2:1181979/1‑141 (MQ=255) gagTTGGATCCCGGTCGTTTGTGGA‑TTTTTTTTAATCCGGGGTTTTGGTTAATATCGCGGTGAGCC‑GGTTTTTTATAAAAGCGGCGGTTCCTATTTTTGTTAGCGCCCAGTCTGGCGCAGCCCGTCTGGTGATTACTCATg > 2:10157/1‑141 (MQ=255) gTTGGATCCCGGTCGGTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGgc < 2:653771/141‑1 (MQ=255) aTCCCGGTCGTGTGTGGA‑TTTTTGTTTATCCGGGTTTTTCGTAAAGATCGCCATGAGCC‑GGTTTTTTATAAAAGCGGCGGTTCCTCTTTTGCTTAGCGCCCAGTCTGGCGCCGCACTTATGGTGATTAATCCAGGCGCGcc > 2:123922/1‑141 (MQ=255) ccGGTCGTTGAGGGA‑TTTTTTTTAAGCCGGGTTATTCGTGAAGTTCGCGTTGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCACGTTTGGTTCGCCCCCAGTAGGGGGCAGCACGGCGGGGGAGTACAACGGGCGCTCCCGt > 2:1389990/1‑141 (MQ=255) gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt < 2:1146952/141‑1 (MQ=255) gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt < 2:519422/141‑1 (MQ=255) gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt < 2:1614310/141‑1 (MQ=255) gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt < 2:1146889/141‑1 (MQ=255) | ATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCT > NC_000913/4412642‑4412914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |