Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,227,218 C→T C18Y (TGC→TAC)  yohF ← putative oxidoreductase YohF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,227,2180CT82.1% 265.4 / 47.2 113C18Y (TGC→TAC) yohFputative oxidoreductase YohF
Reads supporting (aligned to +/- strand):  ref base C (1/19);  new base T (27/65);  total (28/85)
Fisher's exact test for biased strand distribution p-value = 2.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 113 total aligned reads displayed.

GATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCAC  >  NC_000913/2227081‑2227351
                                                                                                                                         |                                                                                                                                     
gATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTAct                                                                                                                                    >  2:369747/1‑141 (MQ=255)
  ttGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTActct                                                                                                                                  >  2:1916461/1‑141 (MQ=255)
  ttGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTActct                                                                                                                                  >  1:1025154/1‑141 (MQ=255)
  ttGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTActct                                                                                                                                  >  1:1025155/1‑141 (MQ=255)
              aGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATcccc                                                                                                                      >  2:812675/1‑141 (MQ=255)
              aGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATcccc                                                                                                                      <  2:105338/141‑1 (MQ=255)
              aGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATcccc                                                                                                                      <  2:105191/141‑1 (MQ=255)
                  gCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAAt                                                                                                                  >  1:1001400/1‑141 (MQ=255)
                   cACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATc                                                                                                                 <  1:1747487/141‑1 (MQ=255)
                    aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCg                                                                                                                <  1:975109/141‑1 (MQ=255)
                    aCGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCg                                                                                                                <  1:975294/141‑1 (MQ=255)
                     cGATCTCCGCTCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACTCGTACTCTTTGCCGATCCCCGAATCgg                                                                                                               <  1:1428874/141‑1 (MQ=255)
                     cGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCgg                                                                                                               <  1:166618/141‑1 (MQ=255)
                               aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTATTAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                                                                                                     >  1:266423/1‑141 (MQ=255)
                               aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGGACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                                                                                                     <  1:673047/141‑1 (MQ=255)
                               aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                                                                                                     <  1:420151/141‑1 (MQ=255)
                               aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                                                                                                     <  1:635398/141‑1 (MQ=255)
                               aCGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTAAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGtaat                                                                                                     <  1:772463/141‑1 (MQ=255)
                                 gTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGtaataa                                                                                                   >  1:1360056/1‑141 (MQ=255)
                                 gTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGtaataa                                                                                                   >  1:1360294/1‑141 (MQ=255)
                                     gCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGc                                                                                               >  2:1522568/1‑141 (MQ=255)
                                     gCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGc                                                                                               >  2:428104/1‑141 (MQ=255)
                                              aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGc                                                                                      >  2:162482/1‑141 (MQ=255)
                                              aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGc                                                                                      <  2:1818620/141‑1 (MQ=255)
                                              aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGc                                                                                      <  1:1706230/141‑1 (MQ=255)
                                                   ccTCCCGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCATTGCCGAGCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  2:1490826/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  2:438895/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  1:1662197/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  2:438871/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  1:1791179/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTGCCCCGTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  1:1178535/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTCCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  2:779682/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTTTTCCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  2:779812/141‑1 (MQ=255)
                                                   ccTCACGCGCGGTATCTATTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCg                                                                                 <  1:1128265/141‑1 (MQ=255)
                                                    cTCCCGCGCGGTATCTTTTGCCCCATCTCCATCTGAGTGCCAGGTAATACCACTATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGa                                                                                <  2:1305538/141‑1 (MQ=255)
                                                    cTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGa                                                                                <  1:1374539/141‑1 (MQ=255)
                                                    cTCACGCGCGGTATCTTTTGCCCCTACTTCATCTGAGTGCCAGGTAATACCACTATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGa                                                                                <  2:1336131/141‑1 (MQ=255)
                                                          cgcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtctc                                                                          <  1:882949/141‑1 (MQ=255)
                                                          cgcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtctc                                                                          <  1:882948/141‑1 (MQ=255)
                                                          cgcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtctc                                                                          <  1:882895/141‑1 (MQ=255)
                                                          cgcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtctc                                                                          >  1:87536/1‑141 (MQ=255)
                                                          cgcgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTtctc                                                                          >  1:87552/1‑141 (MQ=255)
                                                            cgGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCa                                                                        >  2:1947017/1‑141 (MQ=255)
                                                                  cTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAAc                                                                  <  2:720965/141‑1 (MQ=255)
                                                                  cTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAAc                                                                  <  1:994013/141‑1 (MQ=255)
                                                                   ttttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACg                                                                 <  2:1209941/141‑1 (MQ=255)
                                                                   ttttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACg                                                                 <  2:436554/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:1612167/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:1612386/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:364356/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:1615073/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  2:579887/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:1612158/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:93598/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:554890/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:176234/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:1339406/141‑1 (MQ=255)
                                                                    tttGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGc                                                                <  1:446450/141‑1 (MQ=255)
                                                                      tGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTg                                                              >  2:1645881/1‑141 (MQ=255)
                                                                      tGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTg                                                              >  2:1645855/1‑141 (MQ=255)
                                                                            ttcttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATaaa                                                        <  2:290340/141‑1 (MQ=255)
                                                                                tcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGtt                                                    <  1:813824/141‑1 (MQ=255)
                                                                                tcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGtt                                                    <  1:813817/141‑1 (MQ=255)
                                                                                 cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTa                                                   <  1:1556252/141‑1 (MQ=255)
                                                                                 cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTa                                                   <  1:1381786/141‑1 (MQ=255)
                                                                                 cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTa                                                   <  1:1003787/141‑1 (MQ=255)
                                                                                 cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTa                                                   <  1:529857/141‑1 (MQ=255)
                                                                                 cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTa                                                   <  1:978489/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATGACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:1422137/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  2:363574/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:1374877/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:917141/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:1520659/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:1810817/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:1813086/141‑1 (MQ=255)
                                                                                  aTCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCGGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTaa                                                  <  1:923317/141‑1 (MQ=255)
                                                                                   acTGAGTGCCAGGTAATACCACTATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAg                                                 <  1:1493764/140‑1 (MQ=255)
                                                                                        gTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAg                                            >  2:1338578/1‑141 (MQ=255)
                                                                                             aGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGgc                                       >  2:1679115/1‑141 (MQ=255)
                                                                                                tAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCa                                    <  2:1925506/141‑1 (MQ=255)
                                                                                                  aTACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCata                                  >  2:750088/1‑141 (MQ=255)
                                                                                                       aaTATCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATatcatc                             >  1:328475/1‑141 (MQ=255)
                                                                                                        atatCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATAtcatca                            <  2:1352516/141‑1 (MQ=255)
                                                                                                        atatCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATAtcatca                            <  1:1875897/141‑1 (MQ=255)
                                                                                                        atatCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATAtcatca                            <  1:1876068/141‑1 (MQ=255)
                                                                                                        atatCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATAtcatca                            >  2:707106/1‑141 (MQ=255)
                                                                                                         tatCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAg                           >  2:161809/1‑141 (MQ=255)
                                                                                                         tatCAAACCCCTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAg                           >  2:161647/1‑141 (MQ=255)
                                                                                                            cAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGt                        <  1:1856708/141‑1 (MQ=255)
                                                                                                                 cccTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTa                   <  2:684665/141‑1 (MQ=255)
                                                                                                                 cccTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTa                   <  2:325982/141‑1 (MQ=255)
                                                                                                                 cccTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTa                   <  2:684453/141‑1 (MQ=255)
                                                                                                                 cccTGCTGCGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTa                   <  2:325988/141‑1 (MQ=255)
                                                                                                                      ctgcGCCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTAccccc              <  1:1458002/141‑1 (MQ=255)
                                                                                                                        gcgcCAGTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCg            >  2:262279/1‑141 (MQ=255)
                                                                                                                           ccAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGccc         <  1:1158090/141‑1 (MQ=255)
                                                                                                                           ccAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGccc         <  1:1040349/141‑1 (MQ=255)
                                                                                                                              gTAATAACGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAAc      <  1:1947017/141‑1 (MQ=255)
                                                                                                                                aataaCGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGc    <  1:704133/141‑1 (MQ=255)
                                                                                                                                  taaCGCGTACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCAc  >  2:1002405/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCAC  >  NC_000913/2227081‑2227351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: