Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,848 C→T R202C (CGT→TGT)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,8480CT83.9% 345.3 / 52.7 137R202C (CGT→TGT) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (13/9);  new base T (46/69);  total (59/78)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 138 total aligned reads displayed.

TCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCG  >  NC_000913/4181708‑4181984
                                                                                                                                            |                                                                                                                                        
tcttctTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGcc                                                                                                                                          <  1:241899/141‑1 (MQ=255)
          cTCCGACAAAGGTAAAACCCCCGCTTCGGGTAAAGTGCTGTCTAACGCGCGTATCCGCCCTGACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAActgcc                                                                                                                                <  2:1134375/141‑1 (MQ=255)
          cTCCGACAAAGGTAAAACCCACTCGTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAActgcc                                                                                                                                <  2:1134381/141‑1 (MQ=255)
            ccGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAActgcctg                                                                                                                              >  2:1696357/1‑141 (MQ=255)
            ccGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAActgcctg                                                                                                                              <  1:1513552/141‑1 (MQ=255)
              gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCg                                                                                                                            <  1:466274/141‑1 (MQ=255)
              gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCg                                                                                                                            <  1:404119/141‑1 (MQ=255)
              gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                                            <  1:1464355/141‑1 (MQ=255)
              gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                                            <  1:1464356/141‑1 (MQ=255)
                cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGAc                                                                                                                          <  1:707331/141‑1 (MQ=255)
                cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGAc                                                                                                                          <  1:1876949/141‑1 (MQ=255)
                       aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCAtt                                                                                                                   <  2:800974/141‑1 (MQ=255)
                       aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCAtt                                                                                                                   <  1:1294105/141‑1 (MQ=255)
                             cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgc                                                                                                             <  1:618498/141‑1 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1555198/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1555098/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:529119/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            <  1:1627481/141‑1 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:851982/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1610042/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1605373/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1605685/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1605804/1‑141 (MQ=255)
                              aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1408670/1‑141 (MQ=255)
                               ctctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcgc                                                                                                           >  1:316754/1‑141 (MQ=255)
                                 ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                         <  1:404872/141‑1 (MQ=255)
                                 ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                         <  1:404880/141‑1 (MQ=255)
                                    cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                      <  2:1233003/141‑1 (MQ=255)
                                    cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                      <  2:1233018/141‑1 (MQ=255)
                                    cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                      <  2:1797681/141‑1 (MQ=255)
                                         aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                                                                                 <  1:559108/141‑1 (MQ=255)
                                           aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTacac                                                                                               >  1:1750000/1‑141 (MQ=255)
                                              gCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                                            <  1:867620/141‑1 (MQ=255)
                                                     accgcgcgTATCATCCCTTCCCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1702654/139‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  1:1126179/141‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1957184/141‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1955357/141‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1886063/141‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1885806/141‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1531877/141‑1 (MQ=255)
                                                     aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:29749/141‑1 (MQ=255)
                                                      aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    >  2:546919/1‑141 (MQ=255)
                                                      aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    >  2:546938/1‑141 (MQ=255)
                                                      aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    >  1:1859794/1‑141 (MQ=255)
                                                      aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCAACATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    >  1:1567822/1‑141 (MQ=255)
                                                        gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATc                                                                                  >  2:795737/1‑141 (MQ=255)
                                                        gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATc                                                                                  >  2:1265907/1‑141 (MQ=255)
                                                        gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATc                                                                                  >  1:1489508/1‑141 (MQ=255)
                                                           cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACCACCTGTACGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                               >  2:390901/1‑141 (MQ=255)
                                                           cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                               >  1:194310/1‑141 (MQ=255)
                                                                    ccgtACCGCGGTTCCTGGCAGGACTTCGAATACGAACCGAACGACCACCAGTACGTACGTAACGACCGGCGCTGTAAACTGCCTGCGACCATCATTCAGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:708945/138‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:387667/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:231069/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1386421/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:222811/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1605491/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:229985/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:495950/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1864859/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1288370/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:54364/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:795035/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:794980/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  1:1612004/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1099095/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:633385/141‑1 (MQ=255)
                                                                    ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  1:673411/141‑1 (MQ=255)
                                                                    ccTGACCGTGGTTCCTGGCTGGACTTCGAATTCGCTCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1084998/141‑1 (MQ=255)
                                                                     cTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCt                                                                     >  1:12290/1‑141 (MQ=255)
                                                                     cTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCt                                                                     <  2:1750000/141‑1 (MQ=255)
                                                                      ttACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCtt                                                                    >  1:1877625/1‑141 (MQ=255)
                                                                       tACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                                   >  1:809665/1‑141 (MQ=255)
                                                                       tACCGTGGATCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTAACGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                                   >  1:809718/1‑141 (MQ=255)
                                                                          cGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaa                                                                >  1:1361920/1‑141 (MQ=255)
                                                                             ggTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaaa                                                             >  2:1171469/1‑141 (MQ=255)
                                                                                   tggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATc                                                       >  1:1953849/1‑141 (MQ=255)
                                                                                          aCTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGaaa                                                >  1:1168884/1‑141 (MQ=255)
                                                                                          aCTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGaaa                                                >  1:1686126/1‑141 (MQ=255)
                                                                                               gAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGt                                           <  2:1236545/141‑1 (MQ=255)
                                                                                                           aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1555198/141‑1 (MQ=255)
                                                                                                           aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1555305/141‑1 (MQ=255)
                                                                                                           aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1555098/141‑1 (MQ=255)
                                                                                                           aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1248723/141‑1 (MQ=255)
                                                                                                           aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1248728/141‑1 (MQ=255)
                                                                                                           aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1366227/141‑1 (MQ=255)
                                                                                                                cAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGAt                          <  1:370937/141‑1 (MQ=255)
                                                                                                                cAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCACCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGAt                          >  1:184737/1‑141 (MQ=255)
                                                                                                                cAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCACCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGAt                          >  1:184742/1‑141 (MQ=255)
                                                                                                                  aCCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATgg                        >  2:306853/1‑141 (MQ=255)
                                                                                                                  aCCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATgg                        >  2:800698/1‑141 (MQ=255)
                                                                                                                       ttCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTg                   >  2:1533143/1‑141 (MQ=255)
                                                                                                                          gtacgtaTCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTg                <  2:134446/141‑1 (MQ=255)
                                                                                                                          gtacgtaTCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTg                <  2:313115/141‑1 (MQ=255)
                                                                                                                            acgtaTCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGcc              >  1:684071/1‑141 (MQ=255)
                                                                                                                                 tCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAAc         <  2:545097/141‑1 (MQ=255)
                                                                                                                                 tCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAAc         <  1:1352724/141‑1 (MQ=255)
                                                                                                                                 tCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAAc         <  1:1352725/141‑1 (MQ=255)
                                                                                                                                       gTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGc   >  1:883565/1‑141 (MQ=255)
                                                                                                                                        tCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCg  >  2:1283875/1‑141 (MQ=255)
                                                                                                                                        tCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCg  >  1:1756874/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCG  >  NC_000913/4181708‑4181984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: