Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,726,6510TG50.0% ‑4.6 / 52.7 30noncoding (2534/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (3/12);  new base G (2/13);  total (5/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTT  >  NC_000913/2726517‑2726735
                                                                                                                                      |                                                                                    
cgctCCACTCGAACAAGGAGCAGCCCCCCTCAGTACACCAGCGACCACGGCAGATAGGGACCGAACTGACTAACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACATACTTCa                                                                                >  2:576863/4‑141 (MQ=1)
      tcacGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCTCCCACTGCAGATATTGACCGCACTGTCTCACTACGTTCTAAACCCACCTCGCGTAACACTTTAATTGTCTAACAGCCATACCCTTGGGACCTACTTCAGCCCCa                                                                          <  2:2133160/138‑1 (MQ=1)
                    catccccccTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCTAACTGTCTCACGACGTTCTAAACCCAGCTAGCGTACAACTTTAAATGGCTAACAGCCATACCCTTGGGACCTACTTCAGACCCAGGATGTGATGAGcc                                                            <  1:91551/138‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  2:1894597/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  2:1437003/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  2:1167246/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  2:373294/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  2:2388485/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  1:2460713/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  1:2544/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  1:1859935/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  1:1175231/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  1:123491/135‑1 (MQ=1)
                            ctcaaatctcAAACGTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAGCCGACATCGa                                                    <  1:123482/135‑1 (MQ=1)
                              caattCTCCAACGCCCACGGCAGATCGGGACCGAACTGTCTCACGACGTTCTACACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  2:1728662/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  2:2386907/138‑1 (MQ=1)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  2:997146/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  2:286461/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  2:286469/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  2:501188/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  1:2383221/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  1:1690248/138‑1 (MQ=0)
                              caattCTCCAACGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAgg                                                  <  1:1690240/138‑1 (MQ=0)
                                     caaacgtccACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCaaa                                           >  1:2060371/8‑141 (MQ=1)
                                      caacgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAac                                          >  1:892963/4‑141 (MQ=1)
                                      caacgcCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAac                                          >  1:2325868/4‑141 (MQ=1)
                                      aaacgtccACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAac                                          <  2:371697/135‑1 (MQ=1)
                                                                accgTCTCCCGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGc                <  1:1322975/138‑1 (MQ=1)
                                                                             gtgcTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACCGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGAGGTGCCGACATCGAGGTGCCAAACACCGCCGTCGATAGGAACTCTTGGGCGGGATCAGCCTGt   >  2:1605858/4‑141 (MQ=1)
                                                                              ctccaaaCCCAGCACGCGTACCACTTCACATGCCGAACAGCCATACCCTGGGGACCTACTTCAGCACCAGGATGTGCAGAGCCGCCATCGAGGTGCCAAACCCCGCCGTCGATATGACCTCTTGGGCGGTATCAGCCTggt  <  2:1395287/137‑3 (MQ=1)
                                                                                                                                      |                                                                                    
CGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTT  >  NC_000913/2726517‑2726735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑