Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 537,971 A→T G113G (GGA→GGT ybbW → putative allantoin transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913537,9710AT84.9% 470.7 / 65.9 166G113G (GGA→GGTybbWputative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base A (14/11);  new base T (101/39);  total (116/50)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

CTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTT  >  NC_000913/537834‑538108
                                                                                                                                         |                                                                                                                                         
cTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTAtt                                                                                                                                        >  2:2296714/1‑141 (MQ=255)
  cAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTgc                                                                                                                                      >  1:1474404/1‑141 (MQ=255)
     cgcCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTgccgc                                                                                                                                   >  2:2042791/1‑141 (MQ=255)
     cgcCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTgccgc                                                                                                                                   >  2:1915489/1‑141 (MQ=255)
       ccTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCca                                                                                                                                 <  1:2346761/141‑1 (MQ=255)
       ccTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCca                                                                                                                                 <  1:2034336/141‑1 (MQ=255)
        cTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCcat                                                                                                                                <  2:1626202/141‑1 (MQ=255)
                 tGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGtggtt                                                                                                                       >  2:468583/1‑141 (MQ=255)
                  gCCGCGGTAATGGTATTAAACGGTGCTGCGGGCATTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGtggttt                                                                                                                      >  2:388459/1‑141 (MQ=255)
                              gTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGt                                                                                                          >  2:2126701/1‑141 (MQ=255)
                                  tAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTAcg                                                                                                      >  2:1867611/1‑141 (MQ=255)
                                  tAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTAcg                                                                                                      >  1:447286/1‑141 (MQ=255)
                                   aaaCGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTAcgc                                                                                                     >  2:452479/1‑141 (MQ=255)
                                     aCGGTGCTGCGGGCAGTAAAGACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgg                                                                                                   <  2:114784/141‑1 (MQ=255)
                                      cGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCggg                                                                                                  <  2:734151/141‑1 (MQ=255)
                                      cGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCggg                                                                                                  <  1:288501/141‑1 (MQ=255)
                                      cGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCggg                                                                                                  >  2:1823391/1‑141 (MQ=255)
                                      cGGTGCGGCGGGCAGTACATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCggg                                                                                                  <  2:594140/141‑1 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTCTTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  2:2425881/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  1:957902/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  2:1039758/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  2:1593420/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCAGGGGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  1:644376/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTAc                                                                                                       >  1:532197/1‑135 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  2:505366/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  1:2237982/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCgggg                                                                                                 >  2:505771/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGGGCTTCGTTCGGTGTACGTGGTGCACTGTGTGCCGGTGTCTGTCGGGGCGGGCTTGCCGCGGTCAGGGGGGTTGGGGTGCTATGTGACGCgggg                                                                                                 >  2:540988/1‑141 (MQ=255)
                                       ggTGCTGCGGGCAGTAAATACGGTGTGCATTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGAGTATTAAGGGGCGGTATTGCCGCCATCATGTGGTGTGGTTTGCAATGTTACGCgggg                                                                                                 >  2:967383/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:1019658/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  2:628315/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  2:583493/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:1464776/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:1831244/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:2562896/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:1301880/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:2422734/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  2:1807615/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:1254669/1‑141 (MQ=255)
                                        gTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGt                                                                                                >  1:1151293/1‑141 (MQ=255)
                                          gctgcGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCa                                                                                              >  1:2345759/1‑141 (MQ=255)
                                             gcGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTg                                                                                           >  2:2411954/1‑141 (MQ=255)
                                                ggCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                        <  2:692410/141‑1 (MQ=255)
                                                ggCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                        <  1:1616152/141‑1 (MQ=255)
                                                ggCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                        <  1:821033/141‑1 (MQ=255)
                                                ggCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                        <  1:2169986/141‑1 (MQ=255)
                                                ggCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                        <  1:2208489/141‑1 (MQ=255)
                                                ggCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGcc                                                                                        <  1:362814/141‑1 (MQ=255)
                                                  cAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTg                                                                                      >  1:2217233/1‑141 (MQ=255)
                                                  cAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTg                                                                                      >  1:1680327/1‑141 (MQ=255)
                                                        aTACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGAt                                                                                <  1:1340086/141‑1 (MQ=255)
                                                          aCGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTc                                                                              >  1:248798/1‑141 (MQ=255)
                                                          aCGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTc                                                                              >  2:916884/1‑141 (MQ=255)
                                                          aCGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTc                                                                              >  2:456488/1‑141 (MQ=255)
                                                              tgtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          >  1:44467/1‑141 (MQ=255)
                                                              tgtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          >  1:1647480/1‑141 (MQ=255)
                                                              tgtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          >  2:171995/1‑141 (MQ=255)
                                                              tgtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          <  1:1593420/141‑1 (MQ=255)
                                                              tgtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          <  1:1347346/141‑1 (MQ=255)
                                                              tgtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          >  1:1169065/1‑141 (MQ=255)
                                                              tgtgCCTTTTGCAATGATCCTGCGTGCTTCTTACGTTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAt                                                                          <  2:1856679/141‑1 (MQ=255)
                                                               gtgCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGAtt                                                                         >  1:2370425/1‑141 (MQ=255)
                                                                 gCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTgg                                                                       >  1:733633/1‑141 (MQ=255)
                                                                  ccTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGc                                                                      >  1:2480791/1‑141 (MQ=255)
                                                                     tttGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCaaa                                                                   >  1:1041354/1‑141 (MQ=255)
                                                                           aTGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTgg                                                             >  2:2166344/1‑141 (MQ=255)
                                                                                       gCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCGGGCCGTATGGAATGCACTGTAACGACGGCTAACTGGAGGCCATGATTCTGAGGTTCAATATCTGGCCggggatatta                                                 >  1:1485695/1‑134 (MQ=255)
                                                                                         ttcttACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAAc                                               <  1:710498/141‑1 (MQ=255)
                                                                                           cttACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAActc                                             <  2:1306407/141‑1 (MQ=255)
                                                                                                 gtgtACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtg                                       >  2:1966996/1‑141 (MQ=255)
                                                                                                 gtgtACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtg                                       <  1:1265396/141‑1 (MQ=255)
                                                                                                 gtgtACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtg                                       >  1:1551873/1‑141 (MQ=255)
                                                                                                 gtgtACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtg                                       <  1:2445297/141‑1 (MQ=255)
                                                                                                 gtgtACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtg                                       >  2:1689824/1‑141 (MQ=255)
                                                                                                         ggtgCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTc                               >  1:1274677/1‑141 (MQ=255)
                                                                                                           tgCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAc                             <  2:2236331/141‑1 (MQ=255)
                                                                                                           tgCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAc                             <  1:1518474/141‑1 (MQ=255)
                                                                                                           tgCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAc                             <  1:1823391/141‑1 (MQ=255)
                                                                                                           tgCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAc                             >  2:1506446/1‑141 (MQ=255)
                                                                                                           tgCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAc                             >  1:241579/1‑141 (MQ=255)
                                                                                                            gCACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAct                            <  1:2124675/141‑1 (MQ=255)
                                                                                                             cACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCActc                           >  2:868294/1‑141 (MQ=255)
                                                                                                             cACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCActc                           >  2:2049867/1‑141 (MQ=255)
                                                                                                             cACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCActc                           >  2:2538372/1‑141 (MQ=255)
                                                                                                             cACTGTTTCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGAGGTGATTTCActc                           >  2:2134047/1‑141 (MQ=255)
                                                                                                                tGTTTCCCGGATTATTCTGGGGCGGAATTTCCGCGTTCATGTGTTTTGGTTTTCATTCTTACGCGGGGTCACTGGCCTGCTGGTTTCTGATTGGCCATCTCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCTCTCTGt                        <  2:1894450/141‑1 (MQ=255)
                                                                                                                    tCCCGGATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTCGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAgg                    >  1:493387/1‑141 (MQ=255)
                                                                                                                         gATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCttt               >  2:305346/1‑141 (MQ=255)
                                                                                                                         gATTATTAAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCttt               >  2:1029387/1‑141 (MQ=255)
                                                                                                                           ttattaAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtct             >  1:1707747/1‑141 (MQ=255)
                                                                                                                           ttattaAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtct             >  2:2464701/1‑141 (MQ=255)
                                                                                                                           ttattaAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtct             >  2:2342991/1‑141 (MQ=255)
                                                                                                                           ttattaAGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtct             >  2:2234515/1‑141 (MQ=255)
                                                                                                                                 aGGGGCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCg       >  1:290158/1‑141 (MQ=255)
                                                                                                                                  ggggCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCgg      >  2:2116135/1‑141 (MQ=255)
                                                                                                                                  ggggCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCgg      >  2:798914/1‑141 (MQ=255)
                                                                                                                                   gggCGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCggg     >  2:323340/1‑141 (MQ=255)
                                                                                                                                   gggCGGTATTGCCGCCATCATCTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCATAAACGGGCCGGGATTTTTAACTCTCTGTGGTGATGTCAGTCTCTAAGGCCGTTCTATAGCggg     >  2:2401834/1‑141 (MQ=255)
                                                                                                                                     gCGGTATTGCCGCCATCATGGGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCt   >  2:1082501/1‑141 (MQ=255)
                                                                                                                                      cGGTATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCtt  >  1:2114036/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CTCAGCGCCTTTTTCATTGCCGCGGTAATGGTATTAAACGGTGCTGCGGGCAGTAAATACGGTGTGCCTTTTGCCATGATCCTGCGTGCTTCTTACGGTGTACGTGGTGCACTGTTTCCCGGATTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGGCTT  >  NC_000913/537834‑538108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: