Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,563,390 T→G intergenic (+273/‑202) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein YffP/CPZ‑55 prophage; uncharacterized protein YffQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,563,3900TG86.3% 501.2 / 62.1 175intergenic (+273/‑202)yffP/yffQCPZ‑55 prophage; uncharacterized protein YffP/CPZ‑55 prophage; uncharacterized protein YffQ
Reads supporting (aligned to +/- strand):  ref base T (14/10);  new base G (96/55);  total (110/65)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 175 total aligned reads displayed.

CCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAAC  >  NC_000913/2563252‑2563524
                                                                                                                                          |                                                                                                                                      
cccAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGcc                                                                                                                                      >  1:440040/1‑141 (MQ=255)
   aGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCccac                                                                                                                                   >  1:288294/1‑141 (MQ=255)
       aGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCaaa                                                                                                                               <  1:1061311/141‑1 (MQ=255)
          tAGCACCCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGcc                                                                                                                            <  2:1525187/141‑1 (MQ=255)
              aaCCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCcaaca                                                                                                                        >  2:1768835/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAGTTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGAt                                                                                                                    >  1:1191518/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGAt                                                                                                                    >  1:1654465/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGAt                                                                                                                    >  2:1427616/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGAt                                                                                                                    >  1:668290/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGAt                                                                                                                    >  1:303295/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGAt                                                                                                                    >  1:2254910/1‑141 (MQ=255)
                  tACCCGGGCCTTTCTCACAATCCCCGCTGACATTAAATCACCCAACTATCCTTTTTCAGGTGGGAGACAGATGGCCGCCGACGTAGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGAt                                                                                                                    >  2:2516113/1‑141 (MQ=255)
                   aCCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATg                                                                                                                   <  1:45105/141‑1 (MQ=255)
                      cGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGa                                                                                                                >  1:56562/1‑141 (MQ=255)
                      cGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGa                                                                                                                >  1:1921572/1‑141 (MQ=255)
                      cGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGa                                                                                                                >  1:2525550/1‑141 (MQ=255)
                       gggCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAg                                                                                                               <  1:1843420/141‑1 (MQ=255)
                             ttCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAAt                                                                                                         >  1:915578/1‑141 (MQ=255)
                             ttCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAAt                                                                                                         <  1:1367200/141‑1 (MQ=255)
                                     aTCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGAt                                                                                                 >  1:1620879/1‑141 (MQ=255)
                                      tCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATg                                                                                                >  2:451977/1‑141 (MQ=255)
                                            cTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATc                                                                                          <  1:1634909/141‑1 (MQ=255)
                                            cTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATc                                                                                          <  2:232181/141‑1 (MQ=255)
                                                 aTTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCTACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCttt                                                                                     <  2:763889/141‑1 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  1:1633510/1‑141 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  2:627894/1‑141 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  1:843136/1‑141 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  2:2102552/1‑141 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  1:829344/1‑141 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  1:633293/1‑141 (MQ=255)
                                                        cACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCtt                                                                              >  1:633301/1‑141 (MQ=255)
                                                         aCCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTc                                                                             >  1:2436627/1‑141 (MQ=255)
                                                         aCCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTc                                                                             >  2:201411/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTct                                                                            >  2:1390680/1‑140 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  2:1535463/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  2:1460811/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  1:1286467/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  1:1662933/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  1:465494/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  2:2238047/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  2:2451858/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  1:1720879/1‑141 (MQ=255)
                                                          cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCa                                                                            >  1:1662958/1‑141 (MQ=255)
                                                            cATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAgg                                                                          >  2:1674078/1‑141 (MQ=255)
                                                             aTCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGc                                                                         <  2:1601906/141‑1 (MQ=255)
                                                              tCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCa                                                                        >  1:1226916/1‑141 (MQ=255)
                                                              tCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCa                                                                        >  2:1196051/1‑141 (MQ=255)
                                                              tCTTTCCGTTTTCAGGGGTGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGTACATTCTAATCAACGCCCACCCAAGCCAACAAGATGGGAGGTGAATTTTGGGATGCAGATCGCGTTTTCGCTTCAGGct                                                                        >  2:2289922/1‑140 (MQ=255)
                                                                    cGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTg                                                                  >  2:1781368/1‑141 (MQ=255)
                                                                    cGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTg                                                                  >  2:188429/1‑141 (MQ=255)
                                                                        ttCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTa                                                              >  1:1109507/1‑141 (MQ=255)
                                                                        ttCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTa                                                              >  1:534873/1‑141 (MQ=255)
                                                                         tCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTaa                                                             >  2:158652/1‑141 (MQ=255)
                                                                               gggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaat                                                       >  1:1340122/1‑141 (MQ=255)
                                                                               gggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaat                                                       >  1:2323107/1‑141 (MQ=255)
                                                                                ggAGACTGATGGCCTCCGACGTTGCAAAATTGATTAATTTCTTAACATTCAAATCAACGACCACCAAATCCAACAAGATGGGAGTTGAATTTTTTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:942673/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:33100/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:2361626/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:2032191/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:2479618/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:2479580/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:417847/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:1367281/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:210713/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:1955319/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:1929915/141‑1 (MQ=255)
                                                                                ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:1226415/141‑1 (MQ=255)
                                                                                ggAGAATGATGGCCGCCGACGTTGCACAATTGATTAATTTCAGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatc                                                      <  2:446865/141‑1 (MQ=255)
                                                                                  agaCTGATGGCCGCCGACGTTGCACACGTGATTAACTTCTGAACCTACAAACCCACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCGTTTTCCCGACAGGCAATTTTGATTAATtcaatcaa                                                    <  2:1080529/141‑1 (MQ=255)
                                                                                  agaCTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaatcaa                                                    <  2:157039/141‑1 (MQ=255)
                                                                                  agaATGATGGCCGCCGACGTTGCACTATTTATTAATTTCTGAACATTCAGATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCGTTCTGGCATTTTTGTTTAATtcaatcaa                                                    <  2:2370814/141‑1 (MQ=255)
                                                                                     cTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGa                                                 <  2:1892125/141‑1 (MQ=255)
                                                                                     cTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGa                                                 <  2:915578/141‑1 (MQ=255)
                                                                                     cTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGa                                                 <  2:1586253/141‑1 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                >  2:2570447/1‑141 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                <  2:411912/141‑1 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                <  2:813083/141‑1 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                >  1:323165/1‑141 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                >  2:1851318/1‑141 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                >  1:504062/1‑141 (MQ=255)
                                                                                      tGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaa                                                <  2:747220/141‑1 (MQ=255)
                                                                                       gATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGaaa                                               >  1:332370/1‑141 (MQ=255)
                                                                                             cgccgACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGt                                         >  1:1216328/1‑141 (MQ=255)
                                                                                             cgccgACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGt                                         <  2:418958/141‑1 (MQ=255)
                                                                                             cgccgACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGt                                         <  2:2336303/141‑1 (MQ=255)
                                                                                                      tGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaa                                <  2:1654465/141‑1 (MQ=255)
                                                                                                      tGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaa                                >  1:2537406/1‑141 (MQ=255)
                                                                                                      tGCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaa                                >  1:688506/1‑141 (MQ=255)
                                                                                                      tGCACAATTGATTAATTTCTGAACATTAAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGAAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaa                                <  2:531689/141‑1 (MQ=255)
                                                                                                       gCACAATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaac                               <  1:2018100/141‑1 (MQ=255)
                                                                                                        cacaATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaaca                              <  2:2212544/141‑1 (MQ=255)
                                                                                                         acaATTGATTAATTTCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaacaa                             >  2:2529659/1‑141 (MQ=255)
                                                                                                             ttGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATa                         >  1:2424561/1‑141 (MQ=255)
                                                                                                             ttGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATa                         >  1:512530/1‑141 (MQ=255)
                                                                                                             ttGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATATTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATa                         >  1:51809/1‑141 (MQ=255)
                                                                                                                      ttCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGa                >  2:813244/1‑141 (MQ=255)
                                                                                                                      ttCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGa                >  2:1332896/1‑141 (MQ=255)
                                                                                                                      ttCTGAACATTCAAATCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGa                >  2:1332987/1‑141 (MQ=255)
                                                                                                                                   aaTCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGaaa   <  2:303295/141‑1 (MQ=255)
                                                                                                                                    aTCAACGCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAAc  <  2:2452836/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CCCAGGCAGTTAGCAACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTCAAATCAACTCCCACCAAAGCCAACAAGATGGGAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAAC  >  NC_000913/2563252‑2563524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: