Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,412,7810CG41.5% 63.9 / 15.8 41S132C (TCT→TGT) yjfMDUF1190 domain‑containing protein YjfM
Reads supporting (aligned to +/- strand):  ref base C (1/23);  new base G (17/0);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.19e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCT  >  NC_000913/4412651‑4412914
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cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGttttttt                                                                                                                             >  1:1941780/1‑140 (MQ=255)
cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGAtttttt                                                                                                                             >  1:314155/1‑140 (MQ=255)
cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGttttttt                                                                                                                             >  1:314091/1‑140 (MQ=255)
cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTg                                                                                                                             >  1:774559/1‑141 (MQ=255)
cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGAtttttt                                                                                                                             >  1:275469/1‑140 (MQ=255)
            aacGCCAAAACGGCATTTTATGCCGATGTTCCGTAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGtt                                                                                                                 <  2:832338/141‑1 (MQ=255)
              cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGGTTTTTTTTTATCCggggttt                                                                                                               >  1:539647/1‑137 (MQ=255)
                             ttATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTTTTTATGCGGGGTATTGGTTAAGATAGCGa                                                                                                >  1:1634238/1‑141 (MQ=255)
                                  ccGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTTAGCCCGGTGATTCGTAAAGAAAGCGAttatc                                                                                           >  2:116996/1‑137 (MQ=255)
                                      tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                       <  1:1171994/141‑1 (MQ=255)
                                      tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                       <  1:1808322/141‑1 (MQ=255)
                                      tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                       <  1:1808312/141‑1 (MQ=255)
                                      tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                       <  1:173196/141‑1 (MQ=255)
                                      tGTTCCGAAGAAGACGACTCAGCAGCACTGTCAGTCGAAGTACGAAAAGTGCTACTACGACAAGGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtt                                                                                       <  2:882655/141‑1 (MQ=255)
                                        ttCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtttg                                                                                     <  2:1210143/141‑1 (MQ=255)
                                        ttCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCgtttg                                                                                     <  2:1210234/141‑1 (MQ=255)
                                                           gcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGTTTTTTTTTAATACGGGGTATTAGTAAAGATAGCGATGATCCGGTTTTTTTTTAAAGcggcgggtg                                                                  >  1:1829402/1‑138 (MQ=255)
                                                           gcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGGTTTTTTTTTATCCGGGTTTTTGGTTAATATAGCGATGTGCCGTTTGTTTTTAAAAGcggcgggta                                                                  >  1:1829044/1‑138 (MQ=255)
                                                           gcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGGTTTTTTTTTAGCCGGGGTTTTAGGAAAGATAGCGGTTAGCCGTTTGTTTTTAACACcggcgggtg                                                                  >  1:1829329/1‑138 (MQ=255)
                                                           gcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTTTTTAGCCGGGGTTTTGGTAAAGATCGCGAGTTTGCGGGTGTTTATTAAAGcggcgggtg                                                                  >  1:1683935/1‑138 (MQ=255)
                                                                   gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGTTTTTTGTTTATCCCGGGTATTAGTAAATATCGCGATGAGCCGGTTGGTTTTAAAAGCGGCGGGTGCGCTTTTg                                                          >  1:805838/1‑141 (MQ=255)
                                                                   gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGATTTTTGTTAAGACGGGGTATTAGTAAAGATAGCGGTGTGCCGGTTTTTTATAAAAGCGGCGGGTCCACttttt                                                          >  1:349828/1‑140 (MQ=255)
                                                                       gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTc                                                      <  1:887241/141‑1 (MQ=255)
                                                                       gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTc                                                      <  1:887275/141‑1 (MQ=255)
                                                                       gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTGTGATTAGAACAGCGGCGGTTCCTCTTTTGCtgc                                                      <  2:494078/141‑3 (MQ=255)
                                                                        tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcg                                                     <  2:1258321/141‑1 (MQ=255)
                                                                        tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcg                                                     <  2:165958/141‑1 (MQ=255)
                                                                        tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcg                                                     <  2:1259610/141‑1 (MQ=255)
                                                                                     aTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTTTAATCCGGGTTATTAGTAAATATAGCGTTGAGCCGTTTGTTTATAACAACGGCGGTTACTCTTTTTGTTAGCGCCCaggcaggc                                        >  2:1842691/1‑141 (MQ=255)
                                                                                        gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgca                                     <  2:1958566/141‑1 (MQ=255)
                                                                                        gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgca                                     <  2:788503/141‑1 (MQ=255)
                                                                                        gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgca                                     <  2:786356/141‑1 (MQ=255)
                                                                                        gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTACTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgca                                     <  2:788476/141‑1 (MQ=255)
                                                                                          tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcagc                                   <  1:1078625/141‑1 (MQ=255)
                                                                                              acGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACtt                               <  2:1634238/141‑1 (MQ=255)
                                                                                                  cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg                           <  2:414754/141‑1 (MQ=255)
                                                                                                      gTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTGTTAATCCGGGTTATTCGTAAAGATAGCGATGAGCCGTTTGTTTATAAAAGCGGCGGTTACACTTTTGCGTAGCGCCCACTCTGGCGCACCACTTATGGTGAt                       >  2:1602057/1‑141 (MQ=255)
                                                                                                                        ccGGTCGTGTGTGGTTTTTTGTTTATCCGGGTTATTCGTAAATATAGCGATGAGCCGTTTGTTTATAACAGCGGCGGGTGCTCTTTTTCTTAGCGGCCAGTCTTGGGCAGCACAGCTGGGGTTTAATACAGGCGCTCCCGt     >  2:492207/1‑141 (MQ=255)
                                                                                                                        ccGGTCGTGTGTGGATTTTTTTTAAACCGGGTTATTCGTAAAGATCGCGATGTGCCGTTTGGTTATTAAAACGGCCGTTACTATTTTGCATACACCCCAATATTGCCCACCACATCTGGGGATTACACCAGGCCCACCGGt     >  2:1869877/1‑141 (MQ=255)
                                                                                                                           gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGTTGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt  <  2:987806/141‑1 (MQ=255)
                                                                                                                           gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt  <  2:1794194/141‑1 (MQ=255)
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CGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCT  >  NC_000913/4412651‑4412914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑