Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,726,9960A.42.9% ‑6.6 / 20.8 14noncoding (2189/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base . (4/2);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT  >  NC_000913/2726915‑2727103
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tcgttAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCaaa                                                  >  2:1155517/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                               >  2:1737240/4‑141 (MQ=0)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                               >  2:757094/4‑141 (MQ=0)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                               >  1:1055481/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                               >  1:1155687/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                               >  1:656475/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                               >  1:930823/4‑141 (MQ=1)
          gataCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACCTTAGAACATCAAACAATAAATGGTGGTATTTCAAGGTCGGCACCAAGCAGACTGGCGTCCACACTTCAAAGCCTCCCAcc                                        >  2:24138/4‑141 (MQ=1)
                        cacaCTACCCACCAGGCTCTGTCCGCAACCCCGATTAGGGGTCACAGTTAGAACATCATCTCTTCAAGGGTGTTATTTCATGGACGGCTCCACGAATTCTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACAtgt                          <  1:1013208/138‑3 (MQ=0)
                           acgaCCCACCAGACCCTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAgg                       <  2:779635/138‑1 (MQ=1)
                                     aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc             <  1:950745/138‑1 (MQ=0)
                                     aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc             <  1:952833/138‑1 (MQ=0)
                                                gccaCCCGGATTCCGGGTCAACGTTAGAACAACAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt  <  2:340632/138‑1 (MQ=0)
                                                gccaCCCGCATTACGGGTCAACGATACAACATCAACCAACAAAGGGTGGTATTTCAAGGTCGGCTCCAAGCAGACTGGCGTCCACACTTCAAGGCCTCCCACGATTCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt  <  2:1613244/138‑1 (MQ=1)
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TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT  >  NC_000913/2726915‑2727103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: