Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,726,996 | 0 | A | . | 42.9% | ‑6.6 / 20.8 | 14 | noncoding (2189/2904 nt) | rrlG | 23S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base A (4/4); new base . (4/2); total (8/6) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT > NC_000913/2726915‑2727103 | tcgttAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCaaa > 2:1155517/4‑141 (MQ=1) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 2:1737240/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 2:757094/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:1055481/4‑141 (MQ=1) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:1155687/4‑141 (MQ=1) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:656475/4‑141 (MQ=1) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:930823/4‑141 (MQ=1) gataCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACCTTAGAACATCAAACAATA‑AATGGTGGTATTTCAAGGTCGGCACCAAGCAGACTGGCGTCCACACTTCAAAGCCTCCCAcc > 2:24138/4‑141 (MQ=1) cacaCTACCCACCAGGCTCTGTCCGCAACCCCGATTAGGGGTCACAGTTAGAACATCATCTCTTC‑AAGGGTGTTATTTCATGGACGGCTCCACGAATTCTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACAtgt < 1:1013208/138‑3 (MQ=0) acgaCCCACCAGACCCTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAgg < 2:779635/138‑1 (MQ=1) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:950745/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:952833/138‑1 (MQ=0) gccaCCCGGATTCCGGGTCAACGTTAGAACAACAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt < 2:340632/138‑1 (MQ=0) gccaCCCGCATTACGGGTCAACGATACAACATCAACCAACA‑AAGGGTGGTATTTCAAGGTCGGCTCCAAGCAGACTGGCGTCCACACTTCAAGGCCTCCCACGATTCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt < 2:1613244/138‑1 (MQ=1) | TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT > NC_000913/2726915‑2727103 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |