Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0310TC53.3% ‑6.0 / 15.8 18noncoding (2154/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (5/2);  new base C (5/3);  total (12/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAG  >  NC_000913/2726915‑2727160
                                                                                                                     |                                                                                                                                 
tcgttAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCaaa                                                                                                           >  2:1155517/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                        >  2:757094/4‑141 (MQ=0)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                        >  2:1737240/4‑141 (MQ=0)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:1055481/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:930823/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:656475/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:1155687/4‑141 (MQ=1)
          gataCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACCTTAGAACATCAAACAATAAATGGTGGTATTTCAAGGTCGGCACCAAGCAGACTGGCGTCCACACTTCAAAGCCTCCCAcc                                                                                                 >  2:24138/4‑141 (MQ=1)
                        cacaCTACCCACCAGGCTCTGTCCGCAACCCCGATTAGGGGTCACAGTTAGAACATCATCTCTTCAAGGGTGTTATTTCATGGACGGCTCCACGAATTCTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACAtgt                                                                                   <  1:1013208/138‑3 (MQ=0)
                           acgaCCCACCAGACCCTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAgg                                                                                <  2:779635/138‑1 (MQ=1)
                                     aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                      <  1:950745/138‑1 (MQ=0)
                                     aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                      <  1:952833/138‑1 (MQ=0)
                                                gccaCCCGGATTCCGGGTCAACGTTAGAACAACAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt                                                           <  2:340632/138‑1 (MQ=0)
                                                gccaCCCGCATTACGGGTCAACGATACAACATCAACCAACAAAGGGTGGTATTTCAAGGTCGGCTCCAAGCAGACTGGCGTCCACACTTCAAGGCCTCCCACGATTCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt                                                           <  2:1613244/138‑1 (MQ=1)
                                                                                               tgttATTTAAAGGTCGGCTCAAATCAGACTGGCGTCCACACTTCAAAGCCTCCCACATATCATAAACATCAAGGCAGAATGTTCAGAGTCAAGCGATAGTAAAGGTACACGGGGTATTTCCTTATTGCCGCGGGTACTCTg             >  2:1574166/4‑141 (MQ=1)
                                                                                                        aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac    >  1:1511170/7‑141 (MQ=0)
                                                                                                        aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac    >  1:1511128/7‑141 (MQ=0)
                                                                                                          ggacGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACACTGCATCTTCACAg  >  2:1651760/4‑141 (MQ=1)
                                                                                                                     |                                                                                                                                 
TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAG  >  NC_000913/2726915‑2727160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: