Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0330CA66.7% ‑6.7 / 12.4 18noncoding (2152/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (9/3);  total (12/6)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAG  >  NC_000913/2726915‑2727160
                                                                                                                       |                                                                                                                               
tcgttAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCaaa                                                                                                           >  2:1155517/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                        >  2:757094/4‑141 (MQ=0)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                        >  2:1737240/4‑141 (MQ=0)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:1055481/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:930823/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:656475/4‑141 (MQ=1)
   ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                        >  1:1155687/4‑141 (MQ=1)
          gataCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACCTTAGAACATCAAACAATAAATGGTGGTATTTCAAGGTCGGCACCAAGCAGACTGGCGTCCACACTTCAAAGCCTCCCAcc                                                                                                 >  2:24138/4‑141 (MQ=1)
                        cacaCTACCCACCAGGCTCTGTCCGCAACCCCGATTAGGGGTCACAGTTAGAACATCATCTCTTCAAGGGTGTTATTTCATGGACGGCTCCACGAATTCTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACAtgt                                                                                   <  1:1013208/138‑3 (MQ=0)
                           acgaCCCACCAGACCCTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAgg                                                                                <  2:779635/138‑1 (MQ=1)
                                     aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                      <  1:950745/138‑1 (MQ=0)
                                     aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                      <  1:952833/138‑1 (MQ=0)
                                                gccaCCCGGATTCCGGGTCAACGTTAGAACAACAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt                                                           <  2:340632/138‑1 (MQ=0)
                                                gccaCCCGCATTACGGGTCAACGATACAACATCAACCAACAAAGGGTGGTATTTCAAGGTCGGCTCCAAGCAGACTGGCGTCCACACTTCAAGGCCTCCCACGATTCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt                                                           <  2:1613244/138‑1 (MQ=1)
                                                                                               tgttATTTAAAGGTCGGCTCAAATCAGACTGGCGTCCACACTTCAAAGCCTCCCACATATCATAAACATCAAGGCAGAATGTTCAGAGTCAAGCGATAGTAAAGGTACACGGGGTATTTCCTTATTGCCGCGGGTACTCTg             >  2:1574166/4‑141 (MQ=1)
                                                                                                        aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac    >  1:1511170/7‑141 (MQ=0)
                                                                                                        aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac    >  1:1511128/7‑141 (MQ=0)
                                                                                                          ggacGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACACTGCATCTTCACAg  >  2:1651760/4‑141 (MQ=1)
                                                                                                                       |                                                                                                                               
TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAG  >  NC_000913/2726915‑2727160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: