| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,727,078 | 0 | C | G | 46.2% | ‑5.7 / 17.3 | 13 | noncoding (2107/2904 nt) | rrlG | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base G (4/2); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGG > NC_000913/2726942‑2727189 | acgaCCCACCAGACCCTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAgg < 2:779635/138‑1 (MQ=1) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:950745/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:952833/138‑1 (MQ=0) gccaCCCGGATTCCGGGTCAACGTTAGAACAACAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt < 2:340632/138‑1 (MQ=0) gccaCCCGCATTACGGGTCAACGATACAACATCAACCAACA‑AAGGGTGGTATTTCAAGGTCGGCTCCAAGCAGACTGGCGTCCACACTTCAAGGCCTCCCACGATTCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCt < 2:1613244/138‑1 (MQ=1) tgttATTTAAAGGTCGGCTCAAATCAGACTGGCGTCCACACTTCAAAGCCTCCCACATATCATAAACATCAAGGCAGAATGTTCAGAGTCAAGCGATAGTAAAGGTACACGGGGTATTTCCTTATTGCCGCGGGTACTCTg > 2:1574166/4‑141 (MQ=1) aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac > 1:1511128/7‑141 (MQ=0) aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac > 1:1511170/7‑141 (MQ=0) ggacGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGGTACACTGCATCTTCACAg > 2:1651760/4‑141 (MQ=1) tgtcGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGa > 1:1114094/4‑141 (MQ=1) tcaacacTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGtctc < 2:471711/138‑1 (MQ=1) ccccacTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCg > 1:666097/4‑141 (MQ=1) ctacAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGATCACGGTGTCTTTCCGTCTTGCCGCGGGAACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTgg > 2:1147078/4‑141 (MQ=0) | ACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGG > NC_000913/2726942‑2727189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |