Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,037,291	T→C	noncoding (32/76 nt)	alaT →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,037,291	0	T	C	100.0%	42.5 / NA	13	noncoding (32/76 nt)	alaT	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/1);  total (12/1)

AGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTT  >  NC_000913/4037162‑4037385                                                                                                                                  |                                                                                               tctgtagctcagttggttagagcgcacccctgatacgggtgaggtcggcagctcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCagg                                                                                     >  1:547349‑M1/99‑141 (MQ=255)         tcagttggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGc                                                                             >  2:1848642‑M1/91‑141 (MQ=255)         tcagttggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGc                                                                             >  2:78582‑M1/91‑141 (MQ=255)         tcagttggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGc                                                                             >  2:78679‑M1/91‑141 (MQ=255)              tggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTc                                                                        >  1:530626‑M1/86‑141 (MQ=255)                 ttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGAt                                                                     <  1:1363712‑M1/59‑1 (MQ=255)                               tgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                       >  1:418488‑M1/69‑141 (MQ=255)                                                   gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa                                   >  2:1327229‑M1/49‑141 (MQ=255)                                                          tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1661623‑M1/42‑141 (MQ=255)                                                          tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1662491‑M1/42‑141 (MQ=255)                                                          tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1664013‑M1/42‑141 (MQ=255)                                                                                    tcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGtt  >  2:2487‑M1/16‑141 (MQ=255)                                                                                    tcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGtt  >  2:389271‑M1/16‑141 (MQ=255)                                                                                                                                  |                                                                                               AGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTT  >  NC_000913/4037162‑4037385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑