Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,037,308	T→A	noncoding (49/76 nt)	alaT →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,037,308	0	T	A	75.0%	17.3 / 1.0	12	noncoding (49/76 nt)	alaT	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base A (8/1);  total (11/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTT  >  NC_000913/4037170‑4037385                                                                                                                                           |                                                                              tcagttggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGc                                                                             >  2:1848642‑M1/91‑141 (MQ=255) tcagttggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGc                                                                             >  2:78582‑M1/91‑141 (MQ=255) tcagttggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGc                                                                             >  2:78679‑M1/91‑141 (MQ=255)      tggttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTc                                                                        >  1:530626‑M1/86‑141 (MQ=255)         ttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGAt                                                                     <  1:1363712‑M1/59‑1 (MQ=255)                       tgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                       >  1:418488‑M1/69‑141 (MQ=255)                                           gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa                                   >  2:1327229‑M1/49‑141 (MQ=255)                                                  tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1661623‑M1/42‑141 (MQ=255)                                                  tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1662491‑M1/42‑141 (MQ=255)                                                  tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1664013‑M1/42‑141 (MQ=255)                                                                            tcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGtt  >  2:2487‑M1/16‑141 (MQ=255)                                                                            tcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGtt  >  2:389271‑M1/16‑141 (MQ=255)                                                                                                                                           |                                                                              CCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTT  >  NC_000913/4037170‑4037385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑