Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,037,324	A→T	noncoding (65/76 nt)	alaT →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,037,324	0	A	T	85.7%	13.9 / ‑2.8	7	noncoding (65/76 nt)	alaT	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (6/0);  total (7/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTT  >  NC_000913/4037192‑4037385                                                                                                                                     |                                                              tgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                       >  1:418488‑M1/69‑141 (MQ=255)                     gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa                                   >  2:1327229‑M1/49‑141 (MQ=255)                            tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1661623‑M1/42‑141 (MQ=255)                            tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1662491‑M1/42‑141 (MQ=255)                            tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa                            >  2:1664013‑M1/42‑141 (MQ=255)                                                      tcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGtt  >  2:2487‑M1/16‑141 (MQ=255)                                                      tcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGtt  >  2:389271‑M1/16‑141 (MQ=255)                                                                                                                                     |                                                              TGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTT  >  NC_000913/4037192‑4037385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑