Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,849 G→T R202L (CGT→CTT)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,8490GT80.0% 275.6 / 70.5 120R202L (CGT→CTT) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (10/14);  new base T (35/61);  total (45/75)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 120 total aligned reads displayed.

GACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCG  >  NC_000913/4181722‑4181984
                                                                                                                               |                                                                                                                                       
gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                                            <  1:1301184/141‑1 (MQ=255)
gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCg                                                                                                                            <  2:206247/141‑1 (MQ=255)
gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCg                                                                                                                            <  2:344878/141‑1 (MQ=255)
 aCAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGa                                                                                                                           <  1:1380217/141‑1 (MQ=255)
  cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGAc                                                                                                                          <  2:1142670/141‑1 (MQ=255)
  cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGAc                                                                                                                          <  2:1142648/141‑1 (MQ=255)
         aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCAtt                                                                                                                   <  1:271058/141‑1 (MQ=255)
         aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCAtt                                                                                                                   <  2:1816908/141‑1 (MQ=255)
         aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCAtt                                                                                                                   >  1:1659906/1‑141 (MQ=255)
         aaaaCCCACTCTTCGGGTAAAGGGCTGTATAACGCGCGTATCATCCCTTCCCGTGGTTCCTGGCTGGACTTCGACTTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCAtt                                                                                                                   <  2:1294038/141‑1 (MQ=255)
              ccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcg                                                                                                              <  1:585523/141‑1 (MQ=255)
               cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgc                                                                                                             <  1:1309313/141‑1 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTATGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:631557/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTATGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1294042/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGTCCAGCTTTCTgcgcg                                                                                                            >  2:1106043/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1106299/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1106274/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1390759/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1423164/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1423229/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:579658/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:579651/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1443416/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:640433/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:72868/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:641452/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:1692725/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  2:126794/1‑141 (MQ=255)
                aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                            >  1:1716794/1‑141 (MQ=255)
                   ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                         <  2:1749071/141‑1 (MQ=255)
                      cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                      <  1:1900352/141‑1 (MQ=255)
                      cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                      <  2:1953599/141‑1 (MQ=255)
                      cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                                      <  2:195839/141‑1 (MQ=255)
                           aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                                                                                 <  2:1594097/141‑1 (MQ=255)
                           aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGGTCCGGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                                                                                 <  2:1767969/141‑1 (MQ=255)
                           aaaGTGCTGTATAACGCGCGTATCATCCCTGACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                                                                                 <  2:1767952/141‑1 (MQ=255)
                             aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTacac                                                                                               >  2:1785035/1‑141 (MQ=255)
                                 cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACcaca                                                                                           <  1:811127/141‑1 (MQ=255)
                                 cTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACcaca                                                                                           <  1:811171/141‑1 (MQ=255)
                                       tccgctcgCATCATCCCTTACCGCGGTTCCTGGCTGGACTTCGAGTTCGACCCGAAGGACTGCGTGTTCGTGCGTATCGACCGGCGCCGCAAACTGCCGGCCTCGGTGCTGCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1635192/139‑1 (MQ=255)
                                       tccgctcgCATCATCCCTTACCGCGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:895132/139‑1 (MQ=255)
                                       cgcgcacgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:714608/139‑1 (MQ=255)
                                       accgcgcgTATCAGCCCTTACCGTGGTTCCTGGCTGGACTTCGACTTCGCTCCGACGGACAACCTGTTCGTACGTAGCGACCGTCGCCGTAACCTGCCTGCGACCTTCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1095525/139‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTCTCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:377458/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1926901/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1646020/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:50970/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1535856/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:1438977/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  1:746923/141‑1 (MQ=255)
                                       aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                     <  2:399071/141‑1 (MQ=255)
                                        aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    >  1:769269/1‑141 (MQ=255)
                                        aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    <  2:342856/141‑1 (MQ=255)
                                        aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                                    <  2:342847/141‑1 (MQ=255)
                                         cgcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGAt                                                                                   <  1:1787596/141‑1 (MQ=255)
                                         cgcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGAt                                                                                   <  1:1850520/141‑1 (MQ=255)
                                         cgcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGAt                                                                                   <  1:1850528/141‑1 (MQ=255)
                                             cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                               >  2:1413460/1‑141 (MQ=255)
                                             cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                               >  1:366376/1‑141 (MQ=255)
                                                      ccTTCCCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1010285/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:428373/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:428227/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:10997/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1137442/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:10462/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1924101/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:415826/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1838087/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACGACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1257395/141‑1 (MQ=255)
                                                      ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                      <  2:1719149/141‑1 (MQ=255)
                                                         tACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                                   >  1:1781666/1‑141 (MQ=255)
                                                            cGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaa                                                                >  1:1849774/1‑141 (MQ=255)
                                                              tGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaa                                                              >  1:987586/1‑141 (MQ=255)
                                                              tGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaa                                                              >  1:792025/1‑141 (MQ=255)
                                                                  tCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGtt                                                          >  2:1357226/1‑141 (MQ=255)
                                                                          ggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGa                                                  >  1:1009077/1‑141 (MQ=255)
                                                                                    ttCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGAt                                        <  2:366376/141‑1 (MQ=255)
                                                                                    ttCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGAt                                        >  2:1201300/1‑141 (MQ=255)
                                                                                    ttCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGAt                                        >  1:1907028/1‑141 (MQ=255)
                                                                                            gAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCt                                >  1:1551330/1‑141 (MQ=255)
                                                                                             aaGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1326708/141‑1 (MQ=255)
                                                                                             aaGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:579651/141‑1 (MQ=255)
                                                                                             aaGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1776347/141‑1 (MQ=255)
                                                                                             aaGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1777048/141‑1 (MQ=255)
                                                                                             aaGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                               <  2:1280970/141‑1 (MQ=255)
                                                                                                       tGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAAc                     <  2:474215/141‑1 (MQ=255)
                                                                                                        gTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACt                    >  1:1727109/1‑141 (MQ=255)
                                                                                                         ttCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTg                   >  2:138327/1‑141 (MQ=255)
                                                                                                         ttCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTg                   >  2:138318/1‑141 (MQ=255)
                                                                                                         ttCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTg                   >  2:570270/1‑141 (MQ=255)
                                                                                                            gtacgtaTCGACCGTCGCCGTAAACTGCCTGCGACCATCAGTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTg                <  1:1480921/141‑1 (MQ=255)
                                                                                                              acgtaTCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTGCACCACAGAGCAGATTCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGcc              >  1:1954343/1‑141 (MQ=255)
                                                                                                              acgtaTCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGcc              >  1:1655133/1‑141 (MQ=255)
                                                                                                                 taTCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGa           <  1:1166797/141‑1 (MQ=255)
                                                                                                                 taTCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGa           <  1:1784653/141‑1 (MQ=255)
                                                                                                                 taTCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGa           <  1:428372/141‑1 (MQ=255)
                                                                                                                     gACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGc       >  1:36795/1‑141 (MQ=255)
                                                                                                                         gTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGc   >  2:397115/1‑141 (MQ=255)
                                                                                                                         gTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGc   <  2:1754420/141‑1 (MQ=255)
                                                                                                                          tCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCg  >  2:557930/1‑141 (MQ=255)
                                                                                                                               |                                                                                                                                       
GACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGCCTGCG  >  NC_000913/4181722‑4181984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: