Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 701,380 C→T G72E (GGG→GAG)  nagC ← DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913701,3800CT78.7% 123.6 / 31.3 62G72E (GGG→GAG) nagCDNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base C (5/8);  new base T (14/34);  total (19/43)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAG  >  NC_000913/701243‑701519
                                                                                                                                         |                                                                                                                                           
gCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCgg                                                                                                                                          >  2:1099764/1‑141 (MQ=255)
 cAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGTAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGt                                                                                                                                         <  2:1420344/141‑1 (MQ=255)
 cAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGt                                                                                                                                         <  2:983002/141‑1 (MQ=255)
 cAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGt                                                                                                                                         <  2:931226/141‑1 (MQ=255)
 cAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGt                                                                                                                                         <  2:917472/141‑1 (MQ=255)
 cAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGt                                                                                                                                         <  2:498173/141‑1 (MQ=255)
 cAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGt                                                                                                                                         <  1:1480866/141‑1 (MQ=255)
   gCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTgg                                                                                                                                       <  2:818467/141‑1 (MQ=255)
            gTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCctgct                                                                                                                              >  1:1187099/1‑141 (MQ=255)
            gTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCctgct                                                                                                                              >  1:1656241/1‑141 (MQ=255)
            gTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCctgct                                                                                                                              >  1:1656228/1‑141 (MQ=255)
             ttcttcTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCctgctg                                                                                                                             >  2:879338/1‑141 (MQ=255)
             ttcttcTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCctgctg                                                                                                                             >  2:721720/1‑141 (MQ=255)
                   tGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAAc                                                                                                                       <  1:1361026/141‑1 (MQ=255)
                      cAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGAGGGAGATAGCGCGGCGGCCCTCGATGGAGGCCTGCTGATCAActtc                                                                                                                    >  2:905475/1‑141 (MQ=255)
                             ttGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATc                                                                                                             <  1:1756322/141‑1 (MQ=255)
                                  gctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGccc                                                                                                        <  1:1253723/141‑1 (MQ=255)
                                  gctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGccc                                                                                                        >  2:316624/1‑141 (MQ=255)
                                  gctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGccc                                                                                                        <  1:1253724/141‑1 (MQ=255)
                                  gctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGccc                                                                                                        >  1:1728385/1‑141 (MQ=255)
                                      agaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGt                                                                                                    <  2:405864/141‑1 (MQ=255)
                                      agaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGt                                                                                                    <  2:520067/141‑1 (MQ=255)
                                            aaCAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATa                                                                                              >  2:350425/1‑141 (MQ=255)
                                               agagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                                           >  2:1737373/1‑141 (MQ=255)
                                               agagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                                           >  2:1016652/1‑141 (MQ=255)
                                               agagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                                           >  2:946929/1‑141 (MQ=255)
                                                gagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCt                                                                                          >  2:1154431/1‑141 (MQ=255)
                                                     agtgtggCGTCATGACCCCCAACCCGTACGCCGCTGGCGTGGAAATAGCGGGTGACGGTGACGATGGCGATAGCGCGGCGGCCCTCCGTGGAGGCCTGCTGATCAACTTCATCGATCAGCCCGCGGTCGCTAAGCTGAcgc                                                                                     <  2:51518/138‑1 (MQ=255)
                                                     aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAcgc                                                                                     <  2:51678/141‑1 (MQ=255)
                                                     aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAcgc                                                                                     <  2:51683/141‑1 (MQ=255)
                                                          ggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAt                                                                                >  1:1588943/1‑141 (MQ=255)
                                                            cGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAttt                                                                              <  1:954627/141‑1 (MQ=255)
                                                               cATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg                                                                           <  1:1374686/141‑1 (MQ=255)
                                                               cATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg                                                                           <  1:1161502/141‑1 (MQ=255)
                                                                 tGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGtt                                                                         <  1:15802/141‑1 (MQ=255)
                                                                      acCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCt                                                                    >  1:300794/1‑141 (MQ=255)
                                                                        cAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTgg                                                                  <  1:165996/141‑1 (MQ=255)
                                                                         caGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGc                                                                 <  2:375803/140‑1 (MQ=255)
                                                                         aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGc                                                                 <  1:1422166/141‑1 (MQ=255)
                                                                         aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGc                                                                 <  1:226260/141‑1 (MQ=255)
                                                                         aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGc                                                                 <  2:375777/141‑1 (MQ=255)
                                                                             cGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgggg                                                             <  1:694543/141‑1 (MQ=255)
                                                                                            tGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCACGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATATTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGc                                              <  1:1462285/141‑1 (MQ=255)
                                                                                                 aTTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGc                                         <  1:1255847/141‑1 (MQ=255)
                                                                                                              gTGACGATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCcgc                            >  2:1449009/1‑141 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  2:380830/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  1:1330028/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  1:1726698/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  1:1329944/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  2:1891169/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  2:1891150/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       >  2:1889910/1‑141 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  2:1642465/141‑1 (MQ=255)
                                                                                                                   gATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                       <  1:241279/141‑1 (MQ=255)
                                                                                                                    aTGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAtt                      <  1:1781431/141‑1 (MQ=255)
                                                                                                                    aTGGAGATAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAtt                      <  1:1781354/141‑1 (MQ=255)
                                                                                                                        agaTAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGcc                  <  1:806129/141‑1 (MQ=255)
                                                                                                                        agaTAGCGCGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGcc                  <  2:1864516/141‑1 (MQ=255)
                                                                                                                             gcgcGGCGGCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc             >  1:995021/1‑141 (MQ=255)
                                                                                                                                cggcggCCCTCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTgg          <  2:774447/141‑1 (MQ=255)
                                                                                                                                cggcggCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTgg          <  2:147692/141‑1 (MQ=255)
                                                                                                                                cggcggCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTgg          <  2:147467/141‑1 (MQ=255)
                                                                                                                                        cccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAg  <  2:18420/141‑1 (MQ=255)
                                                                                                                                        cccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAg  <  2:18385/141‑1 (MQ=255)
                                                                                                                                        cccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAg  <  2:18428/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAG  >  NC_000913/701243‑701519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: