42C: 42C experiment stats

Notes

Publication:

Sandberg TE, Pedersen M, LaCroix RA, Ebrahim A, Bonde M, Herrgard MJ, et al. Evolution of Escherichia coli to 42 °C and subsequent genetic engineering reveals adaptive mechanisms and novel mutations. Mol Biol Evol. 2014;31: 2647–2662

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
0	1	1
1	1	1
2	1	1
3	1	1
4	1	1
5	1	1
6	1	1
7	1	1
8	1	1
9	1	1
10	1	1
11	11	11

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	120	150
Multiple Base Substitutions	1	1
Deletions	21	42
Insertions	18	23
Mobile Element Insertions	5	12
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	165	228

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	45	67
Noncoding	3	4
Pseudogene	5	6
Synonymous	0	0
Nonsynonymous	0	0
Total	53	77

Mutation Needle Plot

Mutation Targets