PGI: PGI experiment stats

Notes

Publication:

Charusanti P, Conrad TM, Knight EM, Venkataraman K, Fong NL, Xie B, et al. Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene. PLoS Genet. Public Library of Science; 2010;6: e1001186

Long CP, Gonzalez JE, Feist AM, Palsson BO, Antoniewicz MR. Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme in Escherichia coli. Proc Natl Acad Sci U S A. 2018;115: 222–227

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
0	1	1
1	1	1
2	1	1
3	1	1
4	1	1
5	1	1
6	1	1
7	1	1
8	1	1
9	1	1
10	1	1
11	11	11

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	30	39
Multiple Base Substitutions	1	1
Deletions	12	12
Insertions	5	5
Mobile Element Insertions	5	8
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	53	65

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	9	12
Noncoding	0	0
Pseudogene	0	0
Synonymous	0	0
Nonsynonymous	0	0
Total	9	12

Mutation Needle Plot

Mutation Targets