C321: C321 experiment stats

Notes

Publication:

Wannier TM, Kunjapur AM, Rice DP, McDonald MJ, Desai MM, Church GM. Adaptive evolution of genomically recoded Escherichia coli. Proc Natl Acad Sci U S A. 2018;115: 3090–3095.

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
1	1	2
2	1	2
3	1	2
4	1	2
5	1	2
6	1	2
7	1	2
8	1	2
9	1	2
10	1	2
11	1	2
12	1	2
13	1	2
14	1	2
14	14	28

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	1606	16591
Multiple Base Substitutions	14	131
Deletions	113	1451
Insertions	137	919
Mobile Element Insertions	6	29
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	1876	19121

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	260	2235
Noncoding	23	164
Pseudogene	84	1068
Synonymous	0	0
Nonsynonymous	0	0
Total	367	3467

Mutation Needle Plot

Mutation Targets