C321: C321.∆A.earlyfix experiment stats

Notes

Publication:

Wannier TM, Kunjapur AM, Rice DP, McDonald MJ, Desai MM, Church GM. Adaptive evolution of genomically recoded Escherichia coli. Proc Natl Acad Sci U S A. 2018;115: 3090–3095.

ALE, flask, and isolate counts

ALE replicate	Flask count	Isolate count
0	1	1
1	1	2
2	1	2
3	1	2
4	1	2
5	1	2
6	1	2
7	1	2
8	1	2
9	1	2
10	1	2
11	1	2
12	1	2
13	1	2
14	1	2
15	15	29

ALE experiment mutation counts

Mutation Type	Unique	Observed
Single Base Substitutions	1340	19105
Multiple Base Substitutions	9	147
Deletions	119	1752
Insertions	160	1115
Mobile Element Insertions	6	39
Amplifications	0	0
Gene Conversions	0	0
Inversions	0	0
Total	1634	22158

ALE experiment functional change counts

Functional Change Type	Unique	Observed
Intergenic	233	2616
Noncoding	21	108
Pseudogene	72	1190
Synonymous	0	0
Nonsynonymous	0	0
Total	326	3914

Mutation Needle Plot