Notes

Publication: None

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 2
1 4 8
2 4 8
3 4 8
4 5 10
5 4 9
6 4 10
7 4 11
8 15 43
9 4 9
10 5 11
11 54 129

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 97 1754
Multiple Base Substitutions 3 111
Deletions 16 487
Insertions 9 321
Mobile Element Insertions 0 0
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 125 2673

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 17 0 42.9 1517158 91.4% 1386682 144.5
A0 F0 I1 R1 17 0 42.9 1517158 91.4% 1386682 144.5
A1 F9 I1 R1 17 0 79.4 2860930 94.7% 2709300 140.2
A1 F9 I1 R1 17 0 79.4 2860930 94.7% 2709300 140.2
A1 F22 I1 R1 18 0 65.8 2366602 94.8% 2243538 141.0
A1 F22 I1 R1 18 0 65.8 2366602 94.8% 2243538 141.0
A1 F68 I1 R1 19 0 60.9 2122464 94.2% 1999361 141.6
A1 F68 I1 R1 19 0 60.9 2122464 94.2% 1999361 141.6
A1 F94 I1 R1 22 0 49.8 1741778 94.3% 1642496 141.8
A1 F94 I1 R1 22 0 49.8 1741778 94.3% 1642496 141.8
A2 F7 I1 R1 18 0 27.8 999924 94.5% 944928 141.4
A2 F7 I1 R1 18 0 27.8 999924 94.5% 944928 141.4
A2 F18 I1 R1 19 0 24.1 859622 94.0% 808044 143.1
A2 F18 I1 R1 19 0 24.1 859622 94.0% 808044 143.1
A2 F74 I1 R1 22 0 34.8 1210890 93.9% 1137025 142.6
A2 F74 I1 R1 22 0 34.8 1210890 93.9% 1137025 142.6
A2 F101 I1 R1 22 0 40.0 1400210 94.5% 1323198 141.2
A2 F101 I1 R1 22 0 40.0 1400210 94.5% 1323198 141.2
A3 F8 I1 R1 18 0 36.6 1323556 94.6% 1252083 141.2
A3 F8 I1 R1 18 0 36.6 1323556 94.6% 1252083 141.2
A3 F24 I1 R1 20 0 33.3 1199384 94.7% 1135816 140.6
A3 F24 I1 R1 20 0 33.3 1199384 94.7% 1135816 140.6
A3 F71 I1 R1 22 0 42.5 1502764 94.6% 1421614 143.6
A3 F71 I1 R1 22 0 42.5 1502764 94.6% 1421614 143.6
A3 F96 I1 R1 20 0 54.6 1923186 94.8% 1823180 139.7
A3 F96 I1 R1 20 0 54.6 1923186 94.8% 1823180 139.7
A4 F4 I1 R1 17 0 32.4 1141670 89.0% 1016086 148.6
A4 F6 I0 R1 31 0 40.7 1457906 90.0% 1312115 147.3
A4 F6 I1 R1 17 0 41.2 1476706 94.7% 1398440 141.7
A4 F6 I1 R1 17 0 41.2 1476706 94.7% 1398440 141.7
A4 F26 I1 R1 18 0 45.8 1652442 94.6% 1563210 140.6
A4 F26 I1 R1 18 0 45.8 1652442 94.6% 1563210 140.6
A4 F73 I1 R1 19 0 59.7 2078612 94.8% 1970524 141.5
A4 F73 I1 R1 19 0 59.7 2078612 94.8% 1970524 141.5
A4 F100 I1 R1 20 0 53.2 1816442 95.1% 1727436 143.4
A4 F100 I1 R1 20 0 53.2 1816442 95.1% 1727436 143.4
A5 F9 I1 R1 18 0 48.0 1726820 94.1% 1624937 141.3
A5 F9 I1 R1 18 0 48.0 1726820 94.1% 1624937 141.3
A5 F9 I1 R2 17 0 54.6 2010650 88.2% 1773393 147.8
A5 F23 I1 R1 20 0 46.9 1668438 94.0% 1568331 143.6
A5 F23 I1 R1 20 0 46.9 1668438 94.0% 1568331 143.6
A5 F70 I1 R1 21 0 34.3 1196984 94.5% 1131149 141.4
A5 F70 I1 R1 21 0 34.3 1196984 94.5% 1131149 141.4
A5 F95 I1 R1 21 0 58.4 2051072 94.9% 1946467 140.4
A5 F95 I1 R1 21 0 58.4 2051072 94.9% 1946467 140.4
A6 F7 I1 R1 18 0 35.0 1243976 94.7% 1178045 141.0
A6 F7 I1 R1 18 0 35.0 1243976 94.7% 1178045 141.0
A6 F21 I1 R1 17 0 37.6 1357466 94.5% 1282805 141.2
A6 F21 I1 R1 17 0 37.6 1357466 94.5% 1282805 141.2
A6 F76 I1 R1 19 0 39.6 1377188 94.6% 1302819 141.8
A6 F76 I1 R1 19 0 39.6 1377188 94.6% 1302819 141.8
A6 F102 I1 R1 24 0 66.2 2339220 94.4% 2208223 139.9
A6 F102 I1 R1 24 0 66.2 2339220 94.4% 2208223 139.9
A6 F102 I2 R1 23 0 60.6 2096682 94.6% 1983461 142.4
A6 F102 I2 R1 23 0 60.6 2096682 94.6% 1983461 142.4
A7 F7 I1 R1 17 0 60.9 2196714 94.7% 2080288 140.8
A7 F7 I1 R1 17 0 60.9 2196714 94.7% 2080288 140.8
A7 F7 I1 R2 14 0 50.8 1798960 92.6% 1665836 149.0
A7 F26 I1 R1 19 0 46.3 1682450 94.6% 1591597 140.5
A7 F26 I1 R1 19 0 46.3 1682450 94.6% 1591597 140.5
A7 F74 I1 R1 20 0 64.4 2271604 93.9% 2133036 140.9
A7 F74 I1 R1 20 0 64.4 2271604 93.9% 2133036 140.9
A7 F74 I2 R1 19 0 131.5 4629764 93.9% 4347348 140.7
A7 F74 I2 R1 19 0 131.5 4629764 93.9% 4347348 140.7
A7 F101 I1 R1 21 0 59.7 2076202 94.9% 1970315 141.3
A7 F101 I1 R1 21 0 59.7 2076202 94.9% 1970315 141.3
A8 F4 I1 R1 18 0 223.2 8559992 87.0% 7447193 140.1
A8 F6 I0 R1 27 0 48.2 1684932 90.0% 1516438 148.3
A8 F6 I0 R1 27 0 48.2 1684932 90.0% 1516438 148.3
A8 F6 I1 R1 18 0 29.1 1014672 94.7% 960894 141.3
A8 F6 I1 R1 18 0 29.1 1014672 94.7% 960894 141.3
A8 F9 I0 R1 23 0 38.9 1378630 88.6% 1221466 148.7
A8 F9 I0 R1 23 0 38.9 1378630 88.6% 1221466 148.7
A8 F9 I1 R1 20 0 33.7 1169952 95.0% 1111454 141.2
A8 F9 I1 R1 20 0 33.7 1169952 95.0% 1111454 141.2
A8 F11 I1 R1 24 0 137.4 5410678 94.8% 5129322 125.4
A8 F23 I0 R1 29 0 39.7 1391800 89.8% 1249836 148.3
A8 F23 I0 R1 29 0 39.7 1391800 89.8% 1249836 148.3
A8 F23 I1 R1 20 0 23.8 838950 93.2% 781901 142.9
A8 F23 I1 R1 20 0 23.8 838950 93.2% 781901 142.9
A8 F29 I0 R1 32 0 37.4 1321382 89.2% 1178672 148.7
A8 F29 I0 R1 32 0 37.4 1321382 89.2% 1178672 148.7
A8 F29 I1 R1 24 0 93.6 4397600 75.9% 3337778 132.4
A8 F29 I1 R1 24 0 93.6 4397600 75.9% 3337778 132.4
A8 F29 I2 R1 23 0 91.3 4036674 75.1% 3031542 140.9
A8 F29 I2 R1 23 0 91.3 4036674 75.1% 3031542 140.9
A8 F34 I0 R1 33 0 34.5 1234658 88.6% 1093906 148.9
A8 F34 I0 R1 33 0 34.5 1234658 88.6% 1093906 148.9
A8 F34 I1 R1 26 0 24.1 841528 92.8% 780937 143.9
A8 F34 I1 R1 26 0 24.1 841528 92.8% 780937 143.9
A8 F34 I2 R1 25 0 76.7 2803508 92.8% 2601655 136.5
A8 F34 I2 R1 25 0 76.7 2803508 92.8% 2601655 136.5
A8 F34 I3 R1 22 0 160.6 5925712 94.3% 5587946 134.6
A8 F34 I3 R1 22 0 160.6 5925712 94.3% 5587946 134.6
A8 F39 I1 R1 24 0 223.1 8367050 94.6% 7915229 131.7
A8 F39 I2 R1 24 0 134.7 4907092 95.3% 4676458 134.3
A8 F40 I0 R1 23 0 39.3 1381890 89.2% 1232645 148.7
A8 F40 I0 R1 23 0 39.3 1381890 89.2% 1232645 148.7
A8 F40 I1 R1 23 0 91.3 3308928 94.5% 3126936 136.1
A8 F57 I0 R1 79 0 61.5 1961902 96.7% 1897159 149.9
A8 F57 I0 R1 79 0 61.5 1961902 96.7% 1897159 149.9
A8 F57 I1 R1 26 0 103.0 3733936 94.6% 3532303 135.4
A8 F57 I1 R1 26 0 103.0 3733936 94.6% 3532303 135.4
A8 F60 I1 R1 23 0 39.0 1343410 93.4% 1254744 145.1
A8 F62 I1 R1 25 0 209.8 7907456 95.1% 7519990 130.4
A8 F62 I2 R1 25 0 70.4 2611778 95.2% 2486412 132.0
A8 F72 I1 R1 25 0 118.9 4191528 95.5% 4002909 138.5
A8 F99 I1 R1 27 0 107.4 3844252 95.8% 3682793 135.9
A8 F130 I1 R1 27 0 84.7 3265602 94.7% 3092525 127.9
A9 F7 I1 R1 17 0 47.2 1653014 94.5% 1562098 141.0
A9 F7 I1 R1 17 0 47.2 1653014 94.5% 1562098 141.0
A9 F7 I1 R2 18 0 65.2 2131072 95.6% 2037304 148.7
A9 F24 I1 R1 18 0 54.3 1979358 94.3% 1866534 141.4
A9 F24 I1 R1 18 0 54.3 1979358 94.3% 1866534 141.4
A9 F75 I1 R1 21 0 60.9 2110098 94.8% 2000372 141.7
A9 F75 I1 R1 21 0 60.9 2110098 94.8% 2000372 141.7
A9 F101 I1 R1 23 0 42.5 1457892 94.3% 1374792 144.1
A9 F101 I1 R1 23 0 42.5 1457892 94.3% 1374792 144.1
A10 F7 I1 R1 18 0 67.0 2419028 94.8% 2293238 140.3
A10 F7 I1 R1 18 0 67.0 2419028 94.8% 2293238 140.3
A10 F7 I1 R2 17 0 42.2 1409708 97.0% 1367416 148.3
A10 F23 I1 R1 19 0 45.6 1631062 94.6% 1542984 141.1
A10 F23 I1 R1 19 0 45.6 1631062 94.6% 1542984 141.1
A10 F29 I1 R1 20 0 72.4 2486344 94.1% 2339649 144.0
A10 F29 I1 R1 20 0 72.4 2486344 94.1% 2339649 144.0
A10 F76 I1 R1 22 0 48.9 1690474 94.9% 1604259 142.0
A10 F76 I1 R1 22 0 48.9 1690474 94.9% 1604259 142.0
A10 F104 I1 R1 23 0 42.3 1438752 95.3% 1371130 143.4
A10 F104 I1 R1 23 0 42.3 1438752 95.3% 1371130 143.4

Mutation Needle Plot

Mutation Targets

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