Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA5 ← / → uspC	IS1 family protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	7 (0.510)	7/400	NT	100%	intergenic (‑271/‑267)	insA5/uspC	IS1 family protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	15 (1.090)	15/400	NT	100%	intergenic (‑274/‑264)	insA5/uspC	IS1 family protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCA  >  NC_000913/2101520‑2101989                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 tCATGATGAGCATAATGTATCGAATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGAAACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTTTTTTCgtgtttctcaattt                                                                                                                                                                                                     >  2:145018/1‑262 (MQ=255)                tGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATGAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                                                                                                                                                                      >  1:44910/1‑275 (MQ=255)                                        tAATGAAGCCTGAAACCAGACAGGCAAAGACTAAAGCACAGGTCAATATCTTCACAGTACATAAAGAAACCTTGATCGAAACCATTTACACGCACAAAATCTGAAAACAGTACCAGCATAAATGATCCTGCGCTCCAATCAACAACCGTATCAGAATAGAGACTTTATTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTa                                                                                                                                                              <  1:145018/275‑1 (MQ=37)                                         aaTGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGACTAGATACTTTCTTTATGAATTTTCGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCATTCATTTAATTTCTTCATGTTTGAGCCACTTTTTTCTCCCGTa                                                                                                                                                              >  1:94846/1‑274 (MQ=255)                                                   gACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATGAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                                                                                                                   <  2:44910/275‑1 (MQ=255)                                                     cacCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATc                                                                                                                                                 <  1:151531/275‑1 (MQ=255)                                                                   aGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGa                                                                                                                                                                                                             >  1:40235/1‑201 (MQ=255)                                                                    gCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcatt                                                                                                                                                                                              >  1:139205/1‑215 (MQ=255)                                                                    gCCTCAAGCACAGGCCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACACAATCTGAAAAACGTACCAGCATAATTGATCCTGCGCACTACTCAACATCCGTATCAATATAGATACTTTCTTTAGCACTTTTTGTGTTCTTAAGGAAGGTGCGAATAAGCggg                                                                                                                                                                                                                               >  1:62545/1‑182 (MQ=255)                                                                       tCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                                                                                                                         <  2:40235/201‑1 (MQ=255)                                                                        cAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                                                                                                                          <  2:139205/215‑1 (MQ=255)                                                                                                   cATAAAGTAACCGTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAAAGATCCTGGGCACCAATAAACAACCGTACCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGATCCGTTTTTTTCACCCGTAAATGCCTTGAATACGCCTATTTAGACCGTTTTTTCGCCATTTTAGGGGTTATCCCCAGt                                                                                                   >  2:77224/1‑275 (MQ=255)                                                                                                           aaCCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGACTCAGATTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGAAAGGTGCGCATAAGCGGGGAACTTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                                                                                                                   >  1:98338/1‑219 (MQ=255)                                                                                                             ccTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATGAAGGAAGGTGCGAATAAGCGGGGAAAGTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgag                                                                                         <  1:210504/275‑1 (MQ=255)                                                                                                                 gATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaagg                                                                                                                                                                                                                                                 <  2:247207/119‑1 (MQ=255)                                                                                                                      aaGCCATGTAGACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                                                                                                                            <  1:83622/151‑1 (MQ=255)                                                                                                                         ccATTTAACCGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAATGTGGGAATAAGCGGGGTAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACt                                                                             <  1:77224/275‑1 (MQ=255)                                                                                                                                                                      ccTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgaga                                                                                        >  1:99009/1‑219 (MQ=255)                                                                                                                                                                          cgcACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGttt                                                                                                                               >  2:37020/1‑176 (MQ=255)                                                                                                                                                                          cgcACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtctc                                                                                    <  2:99009/219‑1 (MQ=255)                                                                                                                                                                              ccAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                                                                                                                           <  1:37020/176‑1 (MQ=255)                                                                                                                                                                                 aTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGt                     >  1:10532/1‑275 (MQ=255)                                                                                                                                                                                         ccGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATATGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTTAATCAGCCTCttt                                                                                                                                        >  1:221730/1‑152 (MQ=255)                                                                                                                                                                                                aGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCTAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa                                                                                                                   <  1:142434/166‑1 (MQ=255)                                                                                                                                                                                                    tattttttttcttgAGTATTTTTTTTTTTTTTAAGGAAGGTGCGAATAAGCGTGGAAATTTTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAATGTTTTTCCCGTAAATGCCTTGCATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTGTTAGTGAGATCTCTCCCACTGACGAATCATTTGGTCCGCCCGAAACAGGTTGGCAAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCa  <  2:10532/268‑1 (MQ=11)                                                                                                                                                                                                    tAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCa  <  1:133978/275‑1 (MQ=255)                                                                                                                                                                                                       aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAg                                                                                             >  2:223087/1‑181 (MQ=255)                                                                                                                                                                                                           tttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgag                                                                                         <  1:223087/181‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 TCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCA  >  NC_000913/2101520‑2101989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.