BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AC_000091	3,261,134	Δ1,199 bp		insH–[tdcD]	insH, [tdcD]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AC_000091	3261134–3262327	3262332	6–1199	160 [0]	[0] 160	insH–[tdcD]	insH,[tdcD]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AC_000091	= 3261133	0 (0.000)	156 (1.220)	104/170	0.0	100%	intergenic (‑59/‑69)	tdcE/insH	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
*	?	AC_000091	3262333 =	0 (0.000)	156 (1.220)	104/170	0.0	100%	coding (1183/1296 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic

Only 100 of 160 total aligned reads displayed.

GCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCT  >  AC_000091/3261044‑3261220

 cTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAc                                                                                        >  2:3301127‑M1/1‑89 (MQ=255)

  ttATCGCTGGTATCAATATCTTCCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAct                                                                                       <  2:3433750‑M1/90‑3 (MQ=255)

  ttATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAct                                                                                       <  1:1101299‑M1/90‑3 (MQ=255)

  ttATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAct                                                                                       <  2:332782‑M1/90‑3 (MQ=255)

   tATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActt                                                                                      >  2:2741882‑M1/1‑87 (MQ=255)

    aTCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActtt                                                                                     <  1:2101929‑M1/90‑5 (MQ=255)

     tCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttg                                                                                    >  1:218958‑M1/1‑85 (MQ=255)

      cGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgc                                                                                   <  2:98894‑M1/90‑7 (MQ=255)

       gCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcc                                                                                  <  1:729241‑M1/90‑8 (MQ=255)

       gCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcc                                                                                  >  2:2386770‑M1/1‑83 (MQ=255)

         tGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgccta                                                                                >  1:389943‑M1/1‑81 (MQ=255)

         tGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgccta                                                                                <  2:2437548‑M1/90‑10 (MQ=255)

         tGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgccta                                                                                >  2:2746177‑M1/1‑81 (MQ=255)

          ggTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaa                                                                               <  2:3423614‑M1/90‑11 (MQ=255)

           gTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaa                                                                              >  2:2969955‑M1/1‑79 (MQ=255)

           gTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaa                                                                              <  1:1002622‑M1/90‑12 (MQ=255)

            tATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaat                                                                             >  2:1451263‑M1/1‑78 (MQ=255)

            tATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaat                                                                             >  1:1691494‑M1/1‑78 (MQ=255)

               cAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgccttaatgaa                                                                          >  2:2235739‑M1/1‑75 (MQ=255)

                aaTATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaat                                                                         <  2:2456824‑M1/90‑17 (MQ=255)

                aaTATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaat                                                                         <  1:2676866‑M1/90‑17 (MQ=255)

                aaTATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaat                                                                         <  1:2330894‑M1/90‑17 (MQ=255)

                 atatCTACCTTCATGAAAAATAATCTGTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatg                                                                        <  1:514‑M1/90‑18 (MQ=255)

                 atatCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatcaatg                                                                        <  2:2819896‑M1/90‑18 (MQ=255)

                  tatCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatgg                                                                       <  2:751048‑M1/90‑19 (MQ=255)

                   atCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggc                                                                      >  1:707257‑M1/1‑71 (MQ=255)

                    tCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggca                                                                     <  2:1895063‑M1/90‑21 (MQ=255)

                     cTACCTTCATGAAAAATAATCTCTCTACAATACTTCAATTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcat                                                                    <  2:52146‑M1/90‑22 (MQ=255)

                     cTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcat                                                                    <  2:908793‑M1/90‑22 (MQ=255)

                     cTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcat                                                                    >  2:192073‑M1/1‑69 (MQ=255)

                      tACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatc                                                                   >  1:2477712‑M1/1‑68 (MQ=255)

                      tACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatc                                                                   <  2:1525901‑M1/90‑23 (MQ=255)

                         cTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaa                                                                >  2:1467725‑M1/1‑65 (MQ=255)

                         cTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaa                                                                <  1:499576‑M1/90‑26 (MQ=255)

                           tCATGAAAATTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaag                                                              <  1:29727‑M1/90‑28 (MQ=255)

                           tCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaag                                                              <  1:2792495‑M1/90‑28 (MQ=255)

                            cATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagc                                                             >  2:3414813‑M1/1‑62 (MQ=255)

                            cATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagc                                                             <  1:2747530‑M1/90‑29 (MQ=255)

                             aTGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagca                                                            >  1:3225552‑M1/1‑61 (MQ=255)

                                aaaaaTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActtttcctaaatgaatggcatccaaagcaatc                                                         >  1:21088‑M1/1‑58 (MQ=255)

                                aaaaaTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatc                                                         >  1:2200432‑M1/1‑58 (MQ=255)

                                 aaaaTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatca                                                        >  1:845114‑M1/1‑57 (MQ=255)

                                  aaaTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgagtggcatccaaagcaatcat                                                       >  1:124490‑M1/1‑56 (MQ=255)

                                  aaaTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcat                                                       >  2:1951975‑M1/1‑56 (MQ=255)

                                  aaaTAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcat                                                       <  1:1765763‑M1/90‑35 (MQ=255)

                                   aataatCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcatt                                                      >  1:2177448‑M1/1‑55 (MQ=255)

                                    ataatCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattt                                                     <  2:3039840‑M1/90‑37 (MQ=255)

                                    ataatCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattt                                                     >  1:3289340‑M1/1‑54 (MQ=255)

                                     taatCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcatttt                                                    >  1:592422‑M1/1‑53 (MQ=255)

                                      aatCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttt                                                   >  1:386835‑M1/1‑52 (MQ=255)

                                      aatCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttt                                                   <  2:395230‑M1/90‑39 (MQ=255)

                                       atCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcatttttt                                                  <  1:570027‑M1/90‑40 (MQ=255)

                                        tctctcTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttc                                                 <  2:2582541‑M1/90‑41 (MQ=255)

                                           ctctACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttc                                              >  2:2954952‑M1/1‑47 (MQ=255)

                                            tctACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcg                                             >  2:1973565‑M1/1‑46 (MQ=255)

                                              tACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgtt                                           >  1:1846313‑M1/1‑44 (MQ=255)

                                              tACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgtt                                           >  1:974098‑M1/1‑44 (MQ=255)

                                              tACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgtt                                           <  2:2568452‑M1/90‑47 (MQ=255)

                                               aCAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgtta                                          <  2:623496‑M1/90‑48 (MQ=255)

                                               aCAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgtta                                          <  1:1808587‑M1/90‑48 (MQ=255)

                                               aCAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgtta                                          <  1:179618‑M1/90‑48 (MQ=255)

                                               aCAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActatgcctaaatgaatggcatccaaagcaatcattttttcttcgtta                                          <  2:758959‑M1/90‑48 (MQ=255)

                                                cAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttag                                         <  1:1047820‑M1/90‑49 (MQ=255)

                                                 aaTACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagt                                        <  1:2581838‑M1/90‑50 (MQ=255)

                                                  aTACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtc                                       <  1:3069089‑M1/90‑51 (MQ=255)

                                                   tACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcg                                      <  2:3229723‑M1/90‑52 (MQ=255)

                                                      ttCAACTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaa                                   >  2:2678677‑M1/1‑36 (MQ=255)

                                                        cAAGTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaata                                 >  2:1975629‑M1/1‑34 (MQ=255)

                                                          aCTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataac                               <  2:547525‑M1/90‑59 (MQ=255)

                                                           cTAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacg                              >  2:338312‑M1/1‑31 (MQ=255)

                                                            tAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacgg                             >  2:2010149‑M1/1‑30 (MQ=255)

                                                            tAAATTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacgg                             >  1:2480802‑M1/1‑30 (MQ=255)

                                                             aaaTTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggc                            <  1:1487282‑M1/90‑62 (MQ=255)

                                                              aaTTATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggca                           <  1:2367796‑M1/90‑63 (MQ=255)

                                                                 tATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaa                        >  2:1066810‑M1/1‑25 (MQ=255)

                                                                 tATGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaa                        >  1:2876991‑M1/1‑25 (MQ=255)

                                                                  aTGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatnncatccaaagcaatcattttttcttcgttagtcggaataacggcacaaa                       <  2:2280020‑M1/90‑67 (MQ=255)

                                                                   tGCAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaat                      <  2:1120104‑M1/90‑68 (MQ=255)

                                                                     cAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatga                    <  1:3411500‑M1/90‑70 (MQ=255)

                                                                     cAAATTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatga                    >  2:2350220‑M1/1‑21 (MQ=255)

                                                                      aaaTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgac                   <  2:9047‑M1/90‑71 (MQ=255)

                                                                       aaTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgaca                  >  2:575834‑M1/1‑19 (MQ=255)

                                                                       aaTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgaca                  <  1:1442792‑M1/90‑72 (MQ=255)

                                                                       aaTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgaca                  <  2:3101809‑M1/90‑72 (MQ=255)

                                                                       aaTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgaca                  >  1:3178557‑M1/1‑19 (MQ=255)

                                                                        aTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacac                 <  1:962358‑M1/90‑73 (MQ=255)

                                                                        aTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacac                 <  1:1604601‑M1/90‑73 (MQ=255)

                                                                        aTTCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacac                 <  1:1277999‑M1/90‑73 (MQ=255)

                                                                          tCTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgc               >  2:1153284‑M1/1‑16 (MQ=255)

                                                                           cTGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcg              >  1:27189‑M1/1‑15 (MQ=255)

                                                                            tGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgc             >  1:602138‑M1/1‑14 (MQ=255)

                                                                            tGCGGGCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgc             >  2:2503810‑M1/1‑14 (MQ=255)

                                                                               gggCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcatt          <  1:1873194‑M1/90‑80 (MQ=255)

                                                                                ggCGCGTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattt         <  1:2683686‑M1/90‑81 (MQ=255)

                                                                                  cgcgTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattttc       >  1:323253‑M1/1‑8 (MQ=255)

                                                                                  cgcgTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattttc       <  2:1641966‑M1/90‑83 (MQ=255)

                                                                                   gcgTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattttca      <  1:3301776‑M1/90‑84 (MQ=255)

                                                                                   gcgTTAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattttca      >  1:1960456‑M1/1‑7 (MQ=255)

                                                                                      ttAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattttcactg   <  2:528852‑M1/90‑87 (MQ=255)

                                                                                       tAActttgcctaaatgaatggcatccaaagcaatcattttttcttcgttagtcggaataacggcacaaatgacacgcgcattttcactgg  >  1:3096133‑M1/1‑3 (MQ=255)

GCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCT  >  AC_000091/3261044‑3261220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`