BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AC_000091	3,261,134	Δ1,199 bp		insH–[tdcD]	insH, [tdcD]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AC_000091	3261134–3262327	3262332	6–1199	160 [0]	[0] 160	insH–[tdcD]	insH,[tdcD]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AC_000091	= 3261133	0 (0.000)	156 (1.220)	104/170	0.0	100%	intergenic (‑59/‑69)	tdcE/insH	pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
*	?	AC_000091	3262333 =	0 (0.000)	156 (1.220)	104/170	0.0	100%	coding (1183/1296 nt)	tdcD	propionate kinase/acetate kinase C, anaerobic

Only 100 of 160 total aligned reads displayed.

TACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAA  >  AC_000091/3262244‑3262422

cttatcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaac                                                                                           >  2:3327477‑M2/90‑90 (MQ=255)

cttatcgctggtatcaatatctaccttcatgaaaaataatctcgctacaatacttcaactaaattatgcaaattctgcgggcgcgttaac                                                                                           >  2:1986822‑M2/90‑90 (MQ=255)

 ttatcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCt                                                                                          <  1:3310807‑M2/2‑1 (MQ=255)

 ttatcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCt                                                                                          <  1:1101299‑M2/2‑1 (MQ=255)

 ttatcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCt                                                                                          >  2:3419606‑M2/89‑90 (MQ=255)

  tatcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCtt                                                                                         >  2:2741882‑M2/88‑90 (MQ=255)

   atcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCttt                                                                                        >  1:2675724‑M2/87‑90 (MQ=255)

   atcgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCttt                                                                                        <  1:2101929‑M2/4‑1 (MQ=255)

     cgctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGc                                                                                      <  2:98894‑M2/6‑1 (MQ=255)

      gctggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGcc                                                                                     <  2:191672‑M2/7‑1 (MQ=255)

         ggtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTaa                                                                                  <  2:3423614‑M2/10‑1 (MQ=255)

          gtatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTaaa                                                                                 <  1:1002622‑M2/11‑1 (MQ=255)

          gtaccaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTaaa                                                                                 <  2:2442894‑M2/11‑1 (MQ=255)

           tatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaat                                                                                >  1:1691494‑M2/79‑90 (MQ=255)

           tatcaatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaat                                                                                >  2:1451263‑M2/79‑90 (MQ=255)

              caatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTTaatgaa                                                                             >  2:2235739‑M2/76‑90 (MQ=255)

               aatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaatgaat                                                                            <  2:2456824‑M2/16‑1 (MQ=255)

               aatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaatgaat                                                                            <  1:205148‑M2/16‑1 (MQ=255)

               aatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaatgaat                                                                            <  1:2676866‑M2/16‑1 (MQ=255)

               aatatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaatgaat                                                                            <  1:2330894‑M2/16‑1 (MQ=255)

                atatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAaatcaatg                                                                           <  2:2819896‑M2/17‑1 (MQ=255)

                 tatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATgg                                                                          <  2:1476613‑M2/18‑1 (MQ=255)

                 tatctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATgg                                                                          <  2:751048‑M2/18‑1 (MQ=255)

                  atctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGc                                                                         <  2:1216411‑M2/19‑1 (MQ=255)

                  atctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGc                                                                         >  1:707257‑M2/72‑90 (MQ=255)

                    ctaccttcatgaaaaataatctctctacaatacttcaattaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCAt                                                                       <  2:52146‑M2/21‑1 (MQ=255)

                    ctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCAt                                                                       <  2:908793‑M2/21‑1 (MQ=255)

                    ctaccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCAt                                                                       >  2:192073‑M2/70‑90 (MQ=255)

                     taccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATc                                                                      <  2:1525901‑M2/22‑1 (MQ=255)

                     taccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATc                                                                      >  1:2477712‑M2/69‑90 (MQ=255)

                     taccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATc                                                                      >  1:2487465‑M2/69‑90 (MQ=255)

                     taccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATc                                                                      <  1:3040169‑M2/22‑1 (MQ=255)

                       ccttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCa                                                                    >  1:899759‑M2/67‑90 (MQ=255)

                        cttcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCaa                                                                   <  1:499576‑M2/25‑1 (MQ=255)

                          tcatgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAg                                                                 >  1:2843753‑M2/64‑90 (MQ=255)

                            atgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttcaCTTTGCCTAAATGAATGGCATCCAAAGCa                                                               >  2:1955435‑M2/62‑90 (MQ=255)

                            atgaaaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCa                                                               >  1:3225552‑M2/62‑90 (MQ=255)

                                aaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCa                                                           <  2:2512074‑M2/33‑1 (MQ=255)

                                aaaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCa                                                           <  1:557451‑M2/33‑1 (MQ=255)

                                 aaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAGTGGCATCCAAAGCAATCAt                                                          >  1:124490‑M2/57‑90 (MQ=255)

                                 aaataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAt                                                          <  1:1765763‑M2/34‑1 (MQ=255)

                                  aataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAtt                                                         >  1:2177448‑M2/56‑90 (MQ=255)

                                   ataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAttt                                                        >  1:3289340‑M2/55‑90 (MQ=255)

                                   ataatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAttt                                                        <  2:3039840‑M2/36‑1 (MQ=255)

                                    taatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAtttt                                                       <  1:2405384‑M2/37‑1 (MQ=255)

                                    taatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAtttt                                                       >  1:2192633‑M2/54‑90 (MQ=255)

                                    taatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAtttt                                                       >  1:592422‑M2/54‑90 (MQ=255)

                                     aatctctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCAttttt                                                      >  1:386835‑M2/53‑90 (MQ=255)

                                          ctctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTttcttc                                                 <  1:2118492‑M2/43‑1 (MQ=255)

                                           tctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCg                                                <  1:656825‑M2/44‑1 (MQ=255)

                                           tctacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCg                                                >  2:1973565‑M2/47‑90 (MQ=255)

                                             tacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGtt                                              <  2:2568452‑M2/46‑1 (MQ=255)

                                             tacaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGtt                                              >  1:1846313‑M2/45‑90 (MQ=255)

                                              acaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTa                                             <  1:823105‑M2/47‑1 (MQ=255)

                                              acaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTa                                             <  2:623496‑M2/47‑1 (MQ=255)

                                              acaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTa                                             <  1:179618‑M2/47‑1 (MQ=255)

                                              acaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTa                                             <  1:1808587‑M2/47‑1 (MQ=255)

                                              acaatacttcaactaaattatgcaaattctgcgggcgcgttaaCTATGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTa                                             <  2:758959‑M2/47‑1 (MQ=255)

                                               caatacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAg                                            <  1:1047820‑M2/48‑1 (MQ=255)

                                                 atacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTc                                          <  1:3069089‑M2/50‑1 (MQ=255)

                                                 atacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATAAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTc                                          >  2:2415679‑M2/41‑90 (MQ=255)

                                                  tacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCg                                         <  2:3229723‑M2/51‑1 (MQ=255)

                                                  tacttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCg                                         <  1:578947‑M2/51‑1 (MQ=255)

                                                     ttcaactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                                      >  2:2077270‑M2/37‑90 (MQ=255)

                                                       caactaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATa                                    <  2:2963981‑M2/56‑1 (MQ=255)

                                                         actaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAc                                  <  2:547525‑M2/58‑1 (MQ=255)

                                                         actaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAc                                  <  1:312955‑M2/58‑1 (MQ=255)

                                                         actaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAc                                  >  2:1142346‑M2/33‑90 (MQ=255)

                                                          ctaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACg                                 >  2:338312‑M2/32‑90 (MQ=255)

                                                          ctaaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACg                                 >  1:2763304‑M2/32‑90 (MQ=255)

                                                           taaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGAATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACgg                                <  2:3154160‑M2/60‑1 (MQ=255)

                                                            aaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGc                               <  1:1487282‑M2/61‑1 (MQ=255)

                                                            aaattatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGc                               >  2:1061354‑M2/30‑90 (MQ=255)

                                                              attatgccaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGcac                             <  2:3365251‑M2/63‑1 (MQ=255)

                                                                tatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACaa                           >  1:2876991‑M2/26‑90 (MQ=255)

                                                                tatgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACaa                           >  2:1066810‑M2/26‑90 (MQ=255)

                                                                 atgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACaaa                          <  1:349816‑M2/66‑1 (MQ=255)

                                                                  tgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAt                         >  1:2275429‑M2/24‑90 (MQ=255)

                                                                  tgcaaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAt                         <  1:3253997‑M2/67‑1 (MQ=255)

                                                                    caaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGa                       <  2:3021526‑M2/69‑1 (MQ=255)

                                                                    caaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGa                       >  2:2350220‑M2/22‑90 (MQ=255)

                                                                     aaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGac                      <  2:9047‑M2/70‑1 (MQ=255)

                                                                     aaattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGac                      <  1:1957565‑M2/70‑1 (MQ=255)

                                                                      aattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGaca                     >  2:575834‑M2/20‑90 (MQ=255)

                                                                      aattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGaca                     <  2:2288841‑M2/71‑1 (MQ=255)

                                                                      aattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGaca                     <  1:412554‑M2/71‑1 (MQ=255)

                                                                      aattctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGaca                     <  1:1442792‑M2/71‑1 (MQ=255)

                                                                       attctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGacac                    <  1:1277999‑M2/72‑1 (MQ=255)

                                                                       attctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGacac                    >  1:3191892‑M2/19‑90 (MQ=255)

                                                                       attctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGacac                    <  1:1604601‑M2/72‑1 (MQ=255)

                                                                       attctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGacac                    <  1:962358‑M2/72‑1 (MQ=255)

                                                                       attctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGacac                    >  2:2756717‑M2/19‑90 (MQ=255)

                                                                        ttctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACAcg                   >  2:1683420‑M2/18‑90 (MQ=255)

                                                                         tctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACAcgc                  >  2:2727935‑M2/17‑90 (MQ=255)

                                                                          ctgcgggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACAcgcg                 >  1:27189‑M2/16‑90 (MQ=255)

                                                                              gggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCAtt             <  1:1873194‑M2/79‑1 (MQ=255)

                                                                              gggcgcgttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGAAAAAATGACACGCGAAtt             >  2:3438542‑M2/12‑90 (MQ=255)

                                                                                    gttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACt       <  1:271074‑M2/85‑1 (MQ=255)

                                                                                     ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTg      <  2:528852‑M2/86‑1 (MQ=255)

                                                                                       aaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGa    >  2:2599539‑M2/3‑90 (MQ=255)

TACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAA  >  AC_000091/3262244‑3262422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`