New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	= 279782	43 (1.390)	4 (0.140)	4/144	2.9	9.3%	coding (871/1968 nt)	yagF	xylonate dehydratase YagF
	?	NZ_CP009273	= 279774	39 (1.400)					coding (863/1968 nt)	yagF	xylonate dehydratase YagF
Rejected: Frequency below/above cutoff threshold.

GGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/279685‑279782 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggatatccCGCGTGGTGATGCCGCGGCTATCCAGCTCGCTGACCGCGCGCGCCGACTGGCGGGCGATCTCCAGCCACACCGCCTGCCCGGACG  <  NZ_CP009273/279774‑279690                                                                                                                                                                                         GGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCCGTCAGCGAGCCGGATAGCCGCGGCATCACCACGCGG                                                                                              >  2:132742/1‑91       GTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATC                                                                                        >  1:163965/1‑91       GTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATC                                                                                        >  2:756828/1‑91        tcgGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCC                                                                                       <  2:672926/88‑1                                   GCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCCGCGTGGTGATGCCGCGGC                                                                    <  1:284224/83‑1                                       GCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCCGCGGGGTGATGCCGCGGCTATC                                                                <  1:652586/83‑1                                                                    GATAGCCGCGGCATCACCACGCGGGATATCCCGCGTGGTGATGCCGCGGCTATCCAGCTCGCTGACCGCGCGCGCCGACTGGC                                   <  2:652586/83‑1                                                                        GCCGCGGCATCACCACGCGGGTTATCCCGCGTGGTGATGCCGCGGCTATCCAGCTCGCTGACCGCGCGCGCCGACTGGCGGGC                               <  2:284224/83‑1                                                                                             ATATCCCGCGTGGTGATGCCGCGGCTATCCAGCTCGCTGACCGCGCGCGCCGACTGGCGGGCGATCTCCCGCCACACCCCCCGCCCGGACG  >  1:985967/1‑91                                                                                                                                                                                         GGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/279685‑279782 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggatatccCGCGTGGTGATGCCGCGGCTATCCAGCTCGCTGACCGCGCGCGCCGACTGGCGGGCGATCTCCAGCCACACCGCCTGCCCGGACG  <  NZ_CP009273/279774‑279690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.