New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 382371362 (1.270)3 (0.070) 3/226 3.4 4.9% intergenic (+17/‑104) xanP/yicH xanthine/proton symporter XanP/AsmA family protein
?NZ_CP009273 = 3823718 59 (1.320)intergenic (+22/‑99) xanP/yicH xanthine/proton symporter XanP/AsmA family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTAC  >  NZ_CP009273/3823579‑3823846
                                                                                                                                       |                                                                                                                                     
gcCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATa                                                                                                                                   <  2:335793/139‑1 (MQ=255)
          ttGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTAccgcc                                                                                                                          <  2:698638/138‑1 (MQ=255)
          ttGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTAccgcc                                                                                                                          <  1:942594/138‑1 (MQ=255)
            gCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACcgccgc                                                                                                                        <  1:54748/138‑1 (MQ=255)
            gCAGTTGGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACcgccgc                                                                                                                        <  2:31086/138‑1 (MQ=255)
                 ttGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACcgccgccagtg                                                                                                                   >  2:892560/1‑133 (MQ=255)
                 ttGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGtct                                                                                                                   >  1:35362/1‑138 (MQ=255)
                  tGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                 >  1:894086/1‑139 (MQ=255)
                   gCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                 <  1:63250/138‑1 (MQ=255)
                   gCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                 <  2:241265/138‑1 (MQ=255)
                               cTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCAt                                                                                                    >  2:160664/1‑139 (MQ=255)
                               cTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCAt                                                                                                    >  1:917133/1‑139 (MQ=255)
                               cTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTATCACCTCTTTAACCAt                                                                                                    >  2:805/1‑139 (MQ=255)
                                 aaaaaaCCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCCCCAGAAAAACAGTAATTCACTGGGGGCGGTAAGAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTc                                                                                                  <  2:470164/138‑1 (MQ=255)
                                   aaacCCTGCCCCCCTCCGGGTCCCCCGCGGGGGGTATTACGCCCATCGTGCTGAATCTGATTTCCCCCCCAGAAAAACAGTAATTCACTGGGGGCGTTAAGAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTccc                                                                                                <  2:825822/139‑1 (MQ=255)
                                    aaaCCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCg                                                                                               >  1:241168/1‑139 (MQ=255)
                                              ccctccGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGAt                                                                                     <  1:286276/138‑1 (MQ=255)
                                                ctccGGGACCGCCGCGGGGGTTATTACTGCCATCGTGCTGAATTTGTTTTCCCCCCCAAAAAAACAGTAATTCCCTGGCGGCGGTAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTg                                                                                   <  2:515687/139‑1 (MQ=255)
                                                  ccGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAACCAGTAATTCACTGGCGGCGGTAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTgcg                                                                                 <  1:158795/139‑1 (MQ=255)
                                                     ggATCCCCGCGGGCGGTATTACTCCCCTCGTTCTGAATCTGATTTTCCCACCAAAAAAACAGTATTTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGt                                                                              <  1:860203/139‑1 (MQ=255)
                                                        tCGCCGGGGGCGTTATTCTTCCTATCGTCCTGAATCTGATTTCCCCCCCAAAAAAACAGTAATTCGCGGGGGGCGGAAAAAAAATTACCGCCGGAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTaaa                                                                           <  2:894086/139‑1 (MQ=255)
                                                          gccgcGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATc                                                                         >  1:172775/1‑139 (MQ=255)
                                                          gccgcGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATc                                                                         >  1:665437/1‑139 (MQ=255)
                                                          gccgcGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATc                                                                         >  1:515687/1‑139 (MQ=255)
                                                           ccgcGGGCGGTTTTACTCCCATCGTGCTGAATCTGATTTCCCCCCAAGAAAAACAGTAATTCCCTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCg                                                                        <  1:420386/139‑1 (MQ=255)
                                                               gggcggTATTCCTGCCATCGTGCTGAATCTGATTTCCCCCCCAAAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCa                                                                    <  1:671238/139‑1 (MQ=255)
                                                               gggcggTATTACTGCCATCGTGCTGAATCTGATTTCCCCACCAAAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCa                                                                    <  1:662408/139‑1 (MQ=255)
                                                               gcaagttATTCCGGCCATGGTGTTAAATTTGATTTCCCCCCCAAAAAAACAGTATTTCACGGGCGGCGGTAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCa                                                                    <  2:506716/133‑1 (MQ=255)
                                                                 gcggTATTATTGCCATCGTGCTGAATCTGATTTCCCCACCAGAAAAACAGTAATTCATTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATa                                                                  <  1:5691/139‑1 (MQ=255)
                                                                 gcggTATTACTGCCATCGTGCGGAATCTGATTTTCCCCCCAAAAAAACAGTAATTCCCTGGCGGGGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATa                                                                  <  1:888131/139‑1 (MQ=255)
                                                                  cgttATTACTGCCATCGTGCTAAATCTGATTTCCCCACCAGAAAAACATTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATaa                                                                 <  2:241168/136‑1 (MQ=255)
                                                                    ttATTACTCCCACCGTGCTGATTTTGTTTTTCCCACCAGAAAACCAGTAATTCACTGGCGGCGGTAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACt                                                               <  2:169299/138‑1 (MQ=255)
                                                                    gTATTACTGCCATCGTGCTGAATCGGATTTCCCCCCCAAAAAAACAGTAATTCACTGGGGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACt                                                               <  2:790088/139‑1 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTc                                                                                                  >  2:353291/1‑93 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTc                                                                                                  <  1:353291/93‑1 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTactgtc                                                    >  1:506716/1‑134 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  2:731337/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  2:158793/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  1:926842/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  1:916331/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  1:878269/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  2:946247/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  1:743391/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                     >  1:689553/1‑138 (MQ=255)
                                                                               aTCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGc                                                    >  2:661188/1‑139 (MQ=255)
                                                                                 cGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCAt                                                  >  2:256499/1‑139 (MQ=255)
                                                                                 cGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCAt                                                  >  1:88839/1‑139 (MQ=255)
                                                                                 cGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCAt                                                  >  1:70946/1‑139 (MQ=255)
                                                                                     ctaattCTGATTTCCCCACCAGAAAATCATAATTCCACTGGCGGCGTTAAGACATTTCCCGCCGCAGTCTCACCTCTTTATCCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCa                                              <  2:70946/134‑1 (MQ=255)
                                                                                     cgaaaTCTGATTCTCCCCCCAAAAAAACAGTAATACACAGGCGCGGGTAAGATATTTCCCGCCGCAGTCTCACCTCTTTACCCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCa                                              <  2:64702/136‑1 (MQ=255)
                                                                                       gAATCTGATTTCCCCCCCAGAAAAACATTAATTCCCGGGGGGCGGAAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACg                                            <  2:128128/139‑1 (MQ=255)
                                                                                                  tCCCACCAGAAAAAAAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGAc                                 >  1:167156/1‑139 (MQ=255)
                                                                                                                        ccACGGGGGGGGGAAAGAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAgc             <  2:878269/137‑1 (MQ=255)
                                                                                                                          aCTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAgctg           <  1:160664/138‑1 (MQ=255)
                                                                                                                           cgggcggcGGTAAGAAAATTTCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAgctgct         <  2:592084/137‑1 (MQ=255)
                                                                                                                              gcggggGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTg                                                                      <  1:799873/75‑1 (MQ=255)
                                                                                                                              gcggcgGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTg                                                                      >  2:799873/1‑75 (MQ=255)
                                                                                                                              gcggcgGAAAGAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTtct      <  2:214627/139‑1 (MQ=255)
                                                                                                                                 gcggTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTa   >  2:145778/1‑139 (MQ=255)
                                                                                                                                 gcggTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTa   >  1:953236/1‑139 (MQ=255)
                                                                                                                                  cggTAATATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTAc  <  2:665437/139‑1 (MQ=255)
                                                                                                                                     tAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCtt                                                          >  1:906285/1‑80 (MQ=255)
                                                                                                                                     tAAGAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCtt                                                          <  2:906285/80‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GCCGCTGATTTTGCAGTTTGCCCCTGAATGGCTGAAAAACCTGCTCTCCTCCGGGATCGCCGCGGGCGGTATTACTGCCATCGTGCTGAATCTGATTTTCCCACCAGAAAAACAGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTAC  >  NZ_CP009273/3823579‑3823846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.