breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→T P21L (CCG→CTG)  folA → type 3 dihydrofolate reductase
RA 214,743 A→C Y174D (TAC→GAC)  proS ← proline‑‑tRNA ligase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase
RA 4,267,454 C→T R20C (CGC→TGC)  soxR → redox‑sensitive transcriptional activator SoxR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357567 587 7 [0] [0] 4 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 518507 518536 30 2 [0] [1] 2 ybbP/rhsD ABC transporter permease/rhs element protein RhsD
* * ÷ NZ_CP009273 524131 524190 60 3 [1] [1] 2 BW25113_RS24960 hypothetical protein
* * ÷ NZ_CP009273 578977 579071 95 3 [1] [1] 3 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 982708 982728 21 2 [1] [0] 2 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase
* * ÷ NZ_CP009273 993132 993177 46 2 [1] [1] 3 ssuE/elfA NADPH‑dependent FMN reductase/fimbrial protein
* * ÷ NZ_CP009273 1205059 1205074 16 9 [0] [0] 21 BW25113_RS06005 phage tail protein
* * ÷ NZ_CP009273 1214925 1214928 4 4 [0] [0] 3 BW25113_RS06070/BW25113_RS25990 hypothetical protein/autotransporter outer membrane beta‑barrel domain‑containing protein
* * ÷ NZ_CP009273 1320848 1320905 58 2 [1] [1] 2 [yciQ] [yciQ]
* * ÷ NZ_CP009273 1781552 1781562 11 2 [1] [0] 3 ppsA/ppsR phosphoenolpyruvate synthase/posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
* * ÷ NZ_CP009273 1807537 1807567 31 2 [1] [1] 3 BW25113_RS09050/cedA hypothetical protein/cell division activator CedA
* * ÷ NZ_CP009273 2027066 2027107 42 2 [1] [1] 2 drpB/ompC cell division protein DrpB/porin OmpC
* * ÷ NZ_CP009273 2185908 2185923 16 2 [1] [1] 6 yehD/yehE fimbrial protein/YehE family protein
* * ÷ NZ_CP009273 2300310 2300324 15 6 [0] [1] 2 mqo/yojI malate dehydrogenase (quinone)/microcin J25 efflux ABC transporter YojI

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)83 (2.510) 35/166 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =1 (0.030)17 (0.640) 13/134 0.5 97.7% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 0 (0.000)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032611 (0.030)8 (0.300) 8/134 1.1 95.2% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 0 (0.000)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)6 (0.180) 4/164 2.2 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727511 (0.030)36 (1.090) 23/166 0.2 97.3% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP