| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NZ_CP009273 | 22,835 | (TGCTGGGCG)1→2 | coding (445/2817 nt) | ileS → | isoleucine‑‑tRNA ligase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP009273 | 22820 = | 4 (0.130) | 27 (0.990) | 21/152 | 0.2 | 93.7% | coding (430/2817 nt) | ileS | isoleucine‑‑tRNA ligase |
| ? | NZ_CP009273 | = 22828 | 0 (0.000) | coding (438/2817 nt) | ileS | isoleucine‑‑tRNA ligase | |||||
GAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCAT > NZ_CP009273/22735‑22908 | gaAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTggg < 2:566959/90‑1 (MQ=255) ttCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTg < 2:542196/90‑1 (MQ=255) ccgagttccgcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgctgggcgtgc > 2:750295‑M1/81‑90 (MQ=255) ccgccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGc < 2:263390/83‑1 (MQ=255) ccgccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGc > 1:263390/1‑83 (MQ=255) ccgcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGa < 1:130369‑M1/17‑1 (MQ=255) cgcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGAc > 2:252314‑M1/73‑90 (MQ=255) cgcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGAc > 1:331565‑M1/73‑90 (MQ=255) gcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACt < 1:192221‑M1/19‑1 (MQ=255) gcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACt > 2:40754‑M1/72‑90 (MQ=255) gcgccaagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACt < 2:501505‑M1/19‑1 (MQ=255) ccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgc > 2:750295‑M2/1‑80 (MQ=255) caagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTc > 2:142134‑M1/68‑90 (MQ=255) aagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCg < 1:219115‑M1/24‑1 (MQ=255) aagtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCg > 1:143589‑M1/67‑90 (MQ=255) gtgccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCa > 2:347216‑M1/65‑90 (MQ=255) ccGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcga < 1:130369‑M2/90‑18 (MQ=255) cgcgCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgac > 2:252314‑M2/1‑72 (MQ=255) cgcgCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgac > 1:331565‑M2/1‑72 (MQ=255) gcgcCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgact < 1:192221‑M2/90‑20 (MQ=255) gcgcCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgact > 2:40754‑M2/1‑71 (MQ=255) gcgcCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgact < 2:501505‑M2/90‑20 (MQ=255) gccgcgaatacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCAcc > 1:374325‑M1/63‑90 (MQ=255) cAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtc > 2:142134‑M2/1‑67 (MQ=255) aaGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcg > 1:143589‑M2/1‑66 (MQ=255) aaGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcg < 1:219115‑M2/90‑25 (MQ=255) gTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgca > 2:347216‑M2/1‑64 (MQ=255) tacgcggcgacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTg < 1:122181‑M1/36‑1 (MQ=255) gCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacc > 1:374325‑M2/1‑62 (MQ=255) gacccaggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGa < 1:177188‑M1/44‑1 (MQ=255) tACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctg < 1:122181‑M2/90‑37 (MQ=255) ggttgacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCaa > 2:105931‑M1/41‑90 (MQ=255) gACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatgga < 1:177188‑M2/90‑45 (MQ=255) gacggtcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGTACCCGTACCTGACCATGGACTTCAAAACt < 2:189925‑M1/54‑1 (MQ=255) tcaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGc > 2:549954‑M1/32‑90 (MQ=255) ggTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaa > 2:105931‑M2/1‑40 (MQ=255) ccaacgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGc < 1:75837‑M1/59‑1 (MQ=255) acgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCaa < 2:25166‑M1/62‑1 (MQ=255) acgcaaagactttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCaa < 2:543113‑M1/62‑1 (MQ=255) gACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgtacccgtacctgaccatggacttcaaaact < 2:189925‑M2/90‑55 (MQ=255) tCAACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagc > 2:549954‑M2/1‑31 (MQ=255) ccaACGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagc < 1:75837‑M2/89‑60 (MQ=255) aCGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaa < 2:25166‑M2/90‑63 (MQ=255) aCGCAAAGACTTTATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaa < 2:543113‑M2/90‑63 (MQ=255) tttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCcgc < 1:333250‑M1/72‑1 (MQ=255) tttatccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCcgc < 2:225909‑M1/72‑1 (MQ=255) tccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCgcgcgc > 2:377638‑M1/15‑90 (MQ=255) ccgtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCt > 1:838849‑M1/14‑90 (MQ=255) gtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTgg < 2:700328‑M1/79‑1 (MQ=255) gtctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTgg < 2:736088‑M1/79‑1 (MQ=255) tctgggcgtgCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTggg < 2:19875‑M1/80‑1 (MQ=255) tttATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgc < 1:333250‑M2/90‑73 (MQ=255) tttATCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgc < 2:225909‑M2/90‑73 (MQ=255) tCCGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgcgcgc > 2:377638‑M2/1‑14 (MQ=255) ccGTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgcgcgct > 1:838849‑M2/1‑13 (MQ=255) gTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgcgcgctgg < 2:700328‑M2/90‑80 (MQ=255) gTCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgcgcgctgg < 2:736088‑M2/90‑80 (MQ=255) tCTGGGCGTGctgggcgtgctgggcgactggtcgcacccgtacctgaccatggacttcaaaactgaagccaacatcatccgcgcgctggg < 2:19875‑M2/90‑81 (MQ=255) | GAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCAT > NZ_CP009273/22735‑22908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |