breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 129,798 A→T 9.1% I488N (ATC→AAC)  yacH ← DUF3300 domain‑containing protein YacH
RA 238,472 C→G 7.1% A89P (GCC→CCC)  ykfM ← uncharacterized protein YkfM
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 349,859 C→G 5.0% intergenic (+287/‑153) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 1,466,210 G→A 6.1% pseudogene (819/2518 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,529,175 T→C 8.5% pseudogene (1286/2049 nt) rhsE → RhsE protein in rhs element
RA 1,529,180 T→C 8.2% pseudogene (1291/2049 nt) rhsE → RhsE protein in rhs element
RA 1,529,185 C→T 8.1% pseudogene (1296/2049 nt) rhsE → RhsE protein in rhs element
RA 1,529,203 C→A 10.8% pseudogene (1314/2049 nt) rhsE → RhsE protein in rhs element
RA 1,529,218 C→T 21.5% pseudogene (1329/2049 nt) rhsE → RhsE protein in rhs element
RA 1,616,197 C→A 5.8% V145V (GTC→GTA yneK → protein YneK
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,103,900 T→C 5.7% H296H (CAT→CAC mutY → adenine DNA glycosylase
RA 3,253,930 C→A 5.6% W95C (TGG→TGT yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,560,455:1 +G 93.8% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,688,287 A→C 15.0% V296G (GTG→GGG)  bcsC ← cellulose biosynthesis protein BcsC
RA 3,815,809 Δ1 bp 6.1% intergenic (‑41/+54) pyrE ← / ← rph orotate phosphoribosyltransferase/truncated RNase PH
RA 3,815,822 T→G 9.4% intergenic (‑54/+41) pyrE ← / ← rph orotate phosphoribosyltransferase/truncated RNase PH
JC 3,815,859 Δ82 bp 10.9% [rph] [rph]
RA 4,184,809 G→T 41.4% G1189C (GGT→TGT)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 23.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,190 A→G 29.2% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,191 A→C 25.5% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =82 (0.720)32 (0.300) 27/458 NT 30.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 73 (0.690)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780582 (0.720)52 (0.490) 39/458 NT 41.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 73 (0.690)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)68 (0.610) 58/482 NT 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 454269019 (0.170)3 (0.030) 3/472 NT 6.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 73 (0.670)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit