Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 3,559,357 Δ1,629 bp 100% [rtcR][glpG] [rtcR], glpR, glpR, [glpG]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3559357 3560985 1629 102 [0] [1] 103 [rtcR]–[glpG] [rtcR],glpR,glpR,[glpG]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35593560 (0.000)93 (1.310) 75/258 NT 99.5% coding (1089/1599 nt) rtcR sigma 54‑dependent transcriptional regulator of rtcBA expression
?NC_000913 3560986 = 1 (0.010)coding (467/831 nt) glpG rhomboid intramembrane serine protease

Only 100 of 102 total aligned reads displayed.

GCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGTTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTC  >  NC_000913/3559211‑3559504
                                                                                                                                                 |                                                                                                                                                    
gCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGag                                                                                                                                                    <  6:282775‑M1/148‑3 (MQ=255)
 cAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGag                                                                                                                                                    >  8:136504‑M1/1‑145 (MQ=255)
     gATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggt                                                                                                                                               >  7:60836‑M1/1‑141 (MQ=255)
     gATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggt                                                                                                                                               >  7:41681‑M1/1‑141 (MQ=255)
      aTCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggtt                                                                                                                                              >  7:302242‑M1/1‑140 (MQ=255)
         gCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggtta                                                                                                                                             >  8:20311‑M1/1‑137 (MQ=255)
          ccAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaaga                                                                                                                                         <  3:43568‑M1/149‑14 (MQ=255)
           cAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGCAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagag                                                                                                                                        <  6:228520‑M1/149‑15 (MQ=255)
           cAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagag                                                                                                                                        <  3:47167‑M1/149‑15 (MQ=255)
            aGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagagg                                                                                                                                       <  3:59644‑M1/149‑16 (MQ=255)
             ggTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagagga                                                                                                                                      <  2:75421‑M1/149‑17 (MQ=255)
              gTGAGCAGTGATTTTCAGCTTATAGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagagga                                                                                                                                      >  8:17679‑M1/1‑132 (MQ=255)
               tGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggata                                                                                                                                    <  8:107251‑M1/149‑19 (MQ=255)
                 aGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatat                                                                                                                                   <  1:46163‑M1/148‑20 (MQ=255)
                  gCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagcggatatgc                                                                                                                                 <  1:312430‑M1/149‑22 (MQ=255)
                  gCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagagga                                                                                                                                      >  5:196385‑M1/1‑128 (MQ=255)
                  gCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagagga                                                                                                                                      <  6:196385‑M1/144‑17 (MQ=255)
                     gTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGATCTACGCCAGagcaggttaaagaggatatgcat                                                                                                                               >  6:301893‑M1/1‑125 (MQ=255)
                      tGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcat                                                                                                                               >  3:37035‑M1/1‑124 (MQ=255)
                      tGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagc                                                                                                                                                   >  6:208556‑M1/1‑124 (MQ=255)
                      tGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagc                                                                                                                                                   <  5:208556‑M1/127‑4 (MQ=255)
                        aTTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcag                                                                                                                            <  4:211820‑M1/148‑27 (MQ=255)
                         ttttCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagc                                                                                                                           >  7:279230‑M1/1‑121 (MQ=255)
                            tCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgag                                                                                                                        >  6:57540‑M1/1‑118 (MQ=255)
                              aGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaa                                                                                                                      <  4:83890‑M1/148‑33 (MQ=255)
                               gCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagt                                                                                                                    >  7:208832‑M1/1‑115 (MQ=255)
                                 ttATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtg                                                                                                                   >  4:220130‑M1/1‑113 (MQ=255)
                                   aTCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcat                                                                                                                >  8:305938‑M1/1‑111 (MQ=255)
                                      gCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaa                                                                                                             >  7:249319‑M1/1‑108 (MQ=255)
                                      gCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGAACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattac                                                                                                             >  4:123567‑M1/1‑108 (MQ=255)
                                          gAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatat                                                                                                                                   >  4:144210‑M1/1‑104 (MQ=255)
                                          gAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatat                                                                                                                                   <  3:144210‑M1/123‑20 (MQ=255)
                                           aaCGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgt                                                                                                        >  4:120075‑M1/1‑103 (MQ=255)
                                           aaCGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgt                                                                                                        >  4:103190‑M1/1‑103 (MQ=255)
                                             cGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgg                                                                                                      <  2:240740‑M1/149‑49 (MQ=255)
                                                  cgcgATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtga                                                                                                  <  7:17679‑M1/148‑53 (MQ=255)
                                                   gcgATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaag                                                                                                >  3:12595‑M1/1‑95 (MQ=255)
                                                    cgATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagt                                                                                               <  3:3609‑M1/149‑56 (MQ=255)
                                                     gATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagt                                                                                               >  7:209592‑M1/1‑93 (MQ=255)
                                                       tttGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaac                                                                                            <  7:4607‑M1/149‑59 (MQ=255)
                                                       tttGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaac                                                                                            >  4:155255‑M1/1‑91 (MQ=255)
                                                               aGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatttgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaac                                                                                    <  1:9393‑M1/149‑67 (MQ=255)
                                                                    gTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaa                                                                                <  5:57540‑M1/148‑71 (MQ=255)
                                                                    gTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgg                                                                                                      >  8:307162‑M1/1‑78 (MQ=255)
                                                                        ccGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttc                                                                           <  7:124076‑M1/149‑76 (MQ=255)
                                                                         cGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcatgttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttca                                                                          <  5:93046‑M1/149‑77 (MQ=255)
                                                                         cGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttca                                                                          <  3:155255‑M1/149‑77 (MQ=255)
                                                                         cGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttca                                                                          <  8:60836‑M1/149‑77 (MQ=255)
                                                                           aaGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaattttagt                                                                        <  4:12595‑M1/149‑79 (MQ=255)
                                                                           aaGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcaga                                                                        <  1:143641‑M1/149‑79 (MQ=255)
                                                                            aGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtg                                                                       <  8:208832‑M1/149‑80 (MQ=255)
                                                                             ggCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgt                                                                      <  8:209592‑M1/149‑81 (MQ=255)
                                                                             ggCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgt                                                                      <  8:247725‑M1/149‑81 (MQ=255)
                                                                              gCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGTGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgtt                                                                     <  1:218912‑M1/149‑82 (MQ=255)
                                                                              gCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgtt                                                                     <  1:234286‑M1/149‑82 (MQ=255)
                                                                               cAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatctgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttg                                                                    <  7:305938‑M1/149‑83 (MQ=255)
                                                                               cAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaa                                                                                             <  7:313232‑M1/124‑58 (MQ=255)
                                                                               cAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaa                                                                                             >  8:313232‑M1/1‑67 (MQ=255)
                                                                                 aaTTTCGCGAAGATCTGTCCGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgtgtagtgcagtaacgcgtgggtgaagtaacgccagaactcaaatttaagtgttgga                                                                  >  4:66275‑M1/1‑65 (MQ=255)
                                                                                  aTTTCGAGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggat                                                                 <  5:301893‑M1/149‑86 (MQ=255)
                                                                                   tttCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatc                                                                <  7:95164‑M1/149‑87 (MQ=255)
                                                                                       gcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaa                                                             <  3:220130‑M1/148‑90 (MQ=255)
                                                                                       gcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaa                                                             <  8:41681‑M1/148‑90 (MQ=255)
                                                                                        cgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatg                                                           >  4:106817‑M1/1‑58 (MQ=255)
                                                                                         gAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaatgaggttatgcatcagcgagaagtgcattaaagggtggctgaagtaacgcaagaactcaaatttcagtgttggatcgaatgg                                                          >  2:304914‑M1/1‑57 (MQ=255)
                                                                                         gAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatgg                                                          >  3:1869‑M1/1‑57 (MQ=255)
                                                                                          aaGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggc                                                         <  5:289209‑M1/149‑94 (MQ=255)
                                                                                           aGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggc                                                         >  1:33796‑M1/1‑55 (MQ=255)
                                                                                            gATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggcca                                                       <  4:27787‑M1/149‑96 (MQ=255)
                                                                                            gATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggcca                                                       <  3:277975‑M1/149‑96 (MQ=255)
                                                                                             aTCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccag                                                      >  5:45088‑M1/1‑53 (MQ=255)
                                                                                                  tACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccag                                                 >  5:30909‑M1/1‑48 (MQ=255)
                                                                                                  tACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccag                                                      <  1:29418‑M1/144‑97 (MQ=255)
                                                                                                     gcgcgGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagcca                                              >  2:295062‑M1/1‑45 (MQ=255)
                                                                                                        cgGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataa                                           <  8:249319‑M1/149‑108 (MQ=255)
                                                                                                          gATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataaca                                         <  1:295062‑M1/149‑110 (MQ=255)
                                                                                                          gATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttgga                                                                  >  3:128381‑M1/1‑40 (MQ=255)
                                                                                                          gATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttgga                                                                  <  4:128381‑M1/124‑85 (MQ=255)
                                                                                                           aTCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacat                                        <  1:119839‑M1/149‑111 (MQ=255)
                                                                                                             cAATCTCTGGACCTTCATCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatca                                      >  3:42049‑M1/1‑37 (MQ=255)
                                                                                                                  tctGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcc                                 >  7:211114‑M1/1‑32 (MQ=255)
                                                                                                                    tGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttc                                  <  3:106817‑M1/146‑117 (MQ=255)
                                                                                                                    tGGACCTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttc                                  <  6:45088‑M1/146‑117 (MQ=255)
                                                                                                                         cTTCACCCTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgc                          >  3:56818‑M1/1‑25 (MQ=255)
                                                                                                                              cccTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcatatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgaga                     <  8:279230‑M1/149‑130 (MQ=255)
                                                                                                                              cccTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgag                      >  4:130188‑M1/1‑20 (MQ=255)
                                                                                                                               ccTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaa                    >  1:67539‑M1/1‑19 (MQ=255)
                                                                                                                               ccTGCCGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaa                    >  6:174892‑M1/1‑19 (MQ=255)
                                                                                                                                    cGGGTCTACGCCAGagcaggttaaagaggatatgcatcagcgagtagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggcctggccagccataacatcacttcctgatcgccgagaatttgc               <  8:302242‑M1/149‑136 (MQ=255)
                                                                                                                                     gggTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgca              <  4:37035‑M1/149‑137 (MQ=255)
                                                                                                                                     gggTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgca              >  2:269761‑M1/1‑13 (MQ=255)
                                                                                                                                     gggTCTAAGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttcgaacgaatggaaaggccagccataacatcacttcctgatcgccgagaatttgca              >  7:1961‑M1/1‑13 (MQ=255)
                                                                                                                                      ggTCTACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggctcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcat             >  2:98737‑M1/1‑12 (MQ=255)
                                                                                                                                        tCTACGCCAGagcaggttaaagaggatattcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccttatcatcacttcctgatcgccgagaatttgcatgg           <  3:123567‑M1/149‑140 (MQ=255)
                                                                                                                                        tCTACGCCAGagcaggttaaagaggatatgcatcagagagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcatgg           >  5:117413‑M1/1‑10 (MQ=255)
                                                                                                                                          tACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcatggc          >  4:228770‑M1/1‑8 (MQ=255)
                                                                                                                                          tACGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcatggc          >  8:37305‑M1/1‑8 (MQ=255)
                                                                                                                                            cGCCAGagcaggttaaagaggatatgcatcagcgagaagttcatttacgcgtgggtgaagttacgccagaactcatatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcattgcaa        <  8:1961‑M1/148‑143 (MQ=255)
                                                                                                                                            cGCCAGagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcatggcaa        <  3:115197‑M1/148‑143 (MQ=255)
                                                                                                                                                 gagcaggttaaagaggatatgcatcagcgagaagtgcattaacgcgtgggtgaagtaacgccagaactcaaatttcagtgttggatcgaatggccaggccagccataacatcacttcctgatcgccgagaatttgcatggcaataaaca  <  7:136504‑M1/149‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCAGCGATCGCCAGGTGAGCAGTGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGTTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTC  >  NC_000913/3559211‑3559504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: