BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,309,624	Δ3,364 bp		[alsA]–[alsR]	[alsA], alsB, [alsR]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4309624	4312987	3364	144 [1]	[1] 147	[alsA]–[alsR]	[alsA],alsB,[alsR]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4309623	1 (0.010)	138 (1.190)	87/272	0.2	99.3%	coding (1358/1533 nt)	alsA	D‑allose ABC transporter ATPase
*	?	NC_000913	4312988 =	1 (0.010)	138 (1.190)	87/272	0.2	99.3%	coding (4/891 nt)	alsR	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Only 100 of 147 total aligned reads displayed.

TAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATT  >  NC_000913/4312841‑4313136

tctcctcttcgctcatgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgattatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggct                                                                                                                                                       >  2:246728‑M2/148‑148 (MQ=255)

   cctcttcgctcatgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggctcat                                                                                                                                                    >  6:258818‑M2/145‑148 (MQ=255)

    ctcttcgctcatgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcagttagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggctcatc                                                                                                                                                   <  4:242205‑M2/5‑1 (MQ=255)

    ctcttcgctcatgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggctcatca                                                                                                                                                  <  4:57002‑M2/6‑1 (MQ=255)

     tcttcgctcatgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCAc                                                                                                                                                 >  2:342626‑M2/143‑149 (MQ=255)

              atgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggctcatc                                                                                                                                                   >  8:228161‑M2/134‑138 (MQ=255)

               tgtcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGt                                                                                                                                       >  2:73801‑M2/133‑149 (MQ=255)

                 tcatcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTcc                                                                                                                                     >  8:211660‑M2/131‑149 (MQ=255)

                   atcgcgattcgtcaggatatgcgtcagtcgtccttcgcagatctcggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCtt                                                                                                                                   <  4:25939‑M2/21‑1 (MQ=255)

                    tcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTcc                                                                                                                                     >  7:226852‑M2/128‑146 (MQ=255)

                    tcgcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTcc                                                                                                                                     <  8:226852‑M2/19‑1 (MQ=255)

                      gcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaa                                                                                                                                <  3:228261‑M2/24‑1 (MQ=255)

                      gcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaa                                                                                                                                >  2:503960‑M2/126‑149 (MQ=255)

                      gcgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttga                                                                                                                                 <  7:211660‑M2/23‑1 (MQ=255)

                       cgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTc                                                                                                                                      <  6:19036‑M2/18‑1 (MQ=255)

                       cgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTc                                                                                                                                      >  5:19036‑M2/125‑142 (MQ=255)

                       cgattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaat                                                                                                                               <  1:342626‑M2/25‑1 (MQ=255)

                        gattcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaatt                                                                                                                              >  8:87384‑M2/124‑149 (MQ=255)

                         attcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaatt                                                                                                                              >  2:222797‑M2/123‑148 (MQ=255)

                          ttcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaattga                                                                                                                            <  3:438680‑M2/28‑1 (MQ=255)

                          ttcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaattga                                                                                                                            <  7:248424‑M2/28‑1 (MQ=255)

                          ttcgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgaaaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaattga                                                                                                                            <  1:513545‑M2/28‑1 (MQ=255)

                            cgtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaattgaa                                                                                                                           <  3:203206‑M2/29‑1 (MQ=255)

                             gtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTc                                                                                                                                      <  4:13843‑M2/18‑1 (MQ=255)

                             gtcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAg                                                                                                                          <  1:73801‑M2/30‑1 (MQ=255)

                              tcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGAc                                                                                                                        <  6:237332‑M2/32‑1 (MQ=255)

                              tcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGAc                                                                                                                        >  6:409951‑M2/118‑149 (MQ=255)

                              tcaggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttcgcTCATCACGTTCTCTTGTCCTTGAATTGAAGAc                                                                                                                        >  6:5026‑M2/118‑149 (MQ=255)

                                aggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGc                                                                                                                      >  7:279360‑M2/116‑149 (MQ=255)

                                aggatttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGc                                                                                                                      >  8:498925‑M2/116‑149 (MQ=255)

                                    tttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaattga                                                                                                                            >  1:54226‑M2/112‑139 (MQ=255)

                                    tttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaattga                                                                                                                            <  2:54226‑M2/28‑1 (MQ=255)

                                    tttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCAc                                                                                                                   >  5:308077‑M2/112‑148 (MQ=255)

                                    tttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCAc                                                                                                                   >  3:340580‑M2/112‑148 (MQ=255)

                                    tttgcgtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACt                                                                                                                  <  7:87384‑M2/38‑1 (MQ=255)

                                         gtcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTatat                                                                                                              >  8:519367‑M2/107‑148 (MQ=255)

                                          tcagtcgtccttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttga                                                                                                                                 >  6:106370‑M2/106‑128 (MQ=255)

                                                    ttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCCCGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGc                                                                                                     >  1:128662‑M2/96‑146 (MQ=255)

                                                    ttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGc                                                                                                                      <  1:35715‑M2/34‑1 (MQ=255)

                                                    ttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATc                                                                                                             >  7:118656‑M2/96‑138 (MQ=255)

                                                    ttcgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATc                                                                                                             <  8:118656‑M2/43‑1 (MQ=255)

                                                      cgcagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggaagacttttccgtcgtccgccagttggcTCATCACGTTCTGTTGTCCTTGAATTCAAGACGCCACTATATCACCACAGCAAGaa                                                                                                >  8:330587‑M2/94‑149 (MQ=255)

                                                        cagaactcggcgttgcggtcgcagacggtgataatttctggtagttctgatgacaccatcaggatgtcttttccttcgtccgccagttggaTCATCACGCTCTCTTGTCCTTGTATTGAAGACGCCACTATATCACCACAGCAAGAACa                                                                                              <  8:52126‑M2/58‑1 (MQ=255)

                                                        cagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaat                                                                                                                               >  1:224288‑M2/92‑116 (MQ=255)

                                                        cagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttgaat                                                                                                                               <  2:224288‑M2/25‑1 (MQ=255)

                                                        cagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGaa                                                                                                >  2:37458‑M2/92‑147 (MQ=255)

                                                        cagaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGaa                                                                                                >  8:495576‑M2/92‑147 (MQ=255)

                                                         agaacacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCATGAACAg                                                                                             <  2:61709‑M2/59‑1 (MQ=255)

                                                            acacggcgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGaa                                                                                           >  8:154602‑M2/88‑148 (MQ=255)

                                                                gccgatgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtagtccgccagttggcTAATCACGTTCTCTTGTCCTTGATTTGAATACGCCACTATATCACCACAGCAAGAACAGAAAAGAt                                                                                      >  4:191319‑M2/84‑149 (MQ=255)

                                                                    atgcggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTg                                                                                  <  8:279360‑M2/70‑1 (MQ=255)

                                                                        ggtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGa                                                                              >  8:18949‑M2/76‑149 (MQ=255)

                                                                         gtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGaa                                                                                                <  6:62259‑M2/56‑1 (MQ=255)

                                                                         gtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGaa                                                                                                >  5:62259‑M2/75‑130 (MQ=255)

                                                                         gtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGAc                                                                             <  7:495576‑M2/75‑1 (MQ=255)

                                                                         gtcgcagacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGAc                                                                             >  8:393247‑M2/75‑149 (MQ=255)

                                                                              agacggtgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTca                                                                         >  1:385790‑M2/70‑148 (MQ=255)

                                                                                    tgataatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACaaaaa                                                                   >  8:8696‑M2/64‑148 (MQ=255)

                                                                                        aatttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACcaca                                                                                                       >  7:236669‑M2/60‑108 (MQ=255)

                                                                                         atttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaa‑taaa                                                            <  5:136190‑M2/91‑1 (MQ=255)

                                                                                         atttcaggtagttaagatgacagcatcaggatgacttttccttcttcagagagttggcTCATCACGTTCTCTTGTCATTGTATTGAATAGTCCAATATATGACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAaaaaa‑taat                                                            <  5:40928‑M2/91‑2 (MQ=255)

                                                                                          tttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaa‑taaaa                                                           >  4:394429‑M2/58‑149 (MQ=255)

                                                                                           ttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaa‑taaaat                                                          >  3:162172‑M2/57‑149 (MQ=255)

                                                                                           ttcaggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaa‑taaaa                                                           <  4:340580‑M2/92‑1 (MQ=255)

                                                                                              aggtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTta                                                       <  7:498925‑M2/96‑1 (MQ=255)

                                                                                                gtagttcagatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCAC‑ATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTtata                                                     >  2:149542‑M2/52‑148 (MQ=255)

                                                                                                       agatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCttg                                                                                                                                  >  5:499691‑M2/45‑66 (MQ=255)

                                                                                                       agatgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGttta                                              >  8:440976‑M2/45‑149 (MQ=255)

                                                                                                         atgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGtttatt                                            >  3:128039‑M2/43‑149 (MQ=255)

                                                                                                         atgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGtttatt                                            <  1:246728‑M2/107‑1 (MQ=255)

                                                                                                         atgacaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGtttatt                                            >  4:124274‑M2/43‑149 (MQ=255)

                                                                                                            acaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGtttatttta                                         <  6:289853‑M2/110‑1 (MQ=255)

                                                                                                            acaccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGtttatttta                                         <  7:440976‑M2/110‑1 (MQ=255)

                                                                                                             caccatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGtttattttat                                        >  2:500238‑M2/39‑149 (MQ=255)

                                                                                                              accatcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTttattttatt                                       <  5:258818‑M2/112‑1 (MQ=255)

                                                                                                              accatcaggatgacttttccgtcgtccgccagttggcTCATAACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTttattttatt                                       >  3:464486‑M2/38‑149 (MQ=255)

                                                                                                              accatcaggacgacttttccgtcgtccgccagttggcTCATCACGTTCTGTTGTCCTTGAATTCAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTttattttatt                                       <  7:330587‑M2/112‑1 (MQ=255)

                                                                                                                aatcaggatgacttttccttcgtccgccagttggcTCATCCCGTTCTCTTGTCCTTGAATTGAAGACGCAACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGAAGTATCAAAAAAA‑TAAAATTTATAAAGTATATTTTAAtgt                                     >  8:357336‑M2/36‑149 (MQ=255)

                                                                                                                 atcaggatgacttttccttcgtccgccagttggcTCATCACGTTCTCTTGACCTTGAATTGAAGACTCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATtgt                                     >  1:308940‑M2/35‑148 (MQ=255)

                                                                                                                 atcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATtgtg                                    <  7:13‑M2/115‑1 (MQ=255)

                                                                                                                  tcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTttattttatt                                       <  1:106638‑M2/112‑1 (MQ=255)

                                                                                                                  tcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTttattttatt                                       >  2:106638‑M2/34‑145 (MQ=255)

                                                                                                                  tcaggatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATtgtg                                    >  3:452044‑M2/34‑148 (MQ=255)

                                                                                                                      gatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTTAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAATGTTTATTTTATTGTGAACaa                               >  4:266252‑M2/30‑149 (MQ=255)

                                                                                                                      gatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACaa                               >  7:489841‑M2/30‑149 (MQ=255)

                                                                                                                      gatgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACaa                               >  4:177261‑M2/30‑149 (MQ=255)

                                                                                                                        tgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATCGTGAACAATg                             <  1:29727‑M2/122‑1 (MQ=255)

                                                                                                                        tgacttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATCGTGAACAATg                             <  1:37458‑M2/122‑1 (MQ=255)

                                                                                                                            ttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGc                           >  2:255366‑M2/24‑147 (MQ=255)

                                                                                                                            ttttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGc                           >  8:363926‑M2/24‑147 (MQ=255)

                                                                                                                              ttccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAGTAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGa                         <  7:519367‑M2/127‑1 (MQ=255)

                                                                                                                                ccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTg                      <  5:298804‑M2/129‑1 (MQ=255)

                                                                                                                                ccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTg                      <  6:308077‑M2/129‑1 (MQ=255)

                                                                                                                                ccgtcgtccgccagttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTg                      <  7:393247‑M2/129‑1 (MQ=255)

                                                                                                                                            agttggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTa         <  4:203343‑M2/142‑1 (MQ=255)

                                                                                                                                                ggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAttt      >  1:164220‑M2/4‑148 (MQ=255)

                                                                                                                                                ggcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAttt      <  2:336990‑M2/145‑1 (MQ=255)

                                                                                                                                                 gcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCa    <  7:154602‑M2/147‑1 (MQ=255)

                                                                                                                                                 gcTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCa    <  2:385790‑M2/147‑1 (MQ=255)

                                                                                                                                                  cTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAt   <  4:452044‑M2/148‑1 (MQ=255)

TAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAA‑TAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATT  >  NC_000913/4312841‑4313136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`