New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3462350 =	51 (1.290)	5 (0.140)	4/250	4.3	10.2%	coding (377/378 nt) coding (7/588 nt)	gspI gspJ	general secretory pathway component, cryptic putative general secretory pathway component, cryptic
	?	NC_000913	3462401 =	41 (1.120)					coding (58/588 nt)	gspJ	putative general secretory pathway component, cryptic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTA  >  NC_000913/3462203‑3462496                                                                                                                                                    |                                                                                                                                                   agTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTaa                                                                                                                                                   <  4:125562/149‑1 (MQ=255)     gAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGc                                                                                                                                               <  8:114484/149‑1 (MQ=255)      aaCTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGcc                                                                                                                                              <  6:138958/149‑1 (MQ=255)            cgcAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAgg                                                                                                                                        <  4:161376/149‑1 (MQ=255)               aGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGtt                                                                                                                                     >  7:16939/1‑149 (MQ=255)                      tCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAtt                                                                                                                              >  4:155577/1‑149 (MQ=255)                      tCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAt                                                                                                                               <  8:16939/148‑1 (MQ=255)                        cACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTgg                                                                                                                            <  3:155577/149‑2 (MQ=255)                             aaGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACACAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAg                                                                                                                       >  3:122410/1‑149 (MQ=255)                                    gACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAgg                                                                                                                                        >  1:183187/1‑125 (MQ=255)                                    gACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAgg                                                                                                                                        <  2:183187/125‑1 (MQ=255)                                       ggCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggcgg                                                                                                             >  4:104630/1‑149 (MQ=255)                                                     aCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                                                                               <  2:165487/149‑1 (MQ=255)                                                         aCCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCaa                                                                                           >  1:46051/1‑149 (MQ=255)                                                               aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTa                                                                                     <  2:46051/149‑1 (MQ=255)                                                                       aTTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                                                                             >  7:61764/1‑149 (MQ=255)                                                                       aTTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                                                                             >  4:130769/1‑149 (MQ=255)                                                                           cccAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGt                                                                         <  3:130769/149‑1 (MQ=255)                                                                            ccAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATCGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                                                                        >  8:107908/1‑149 (MQ=255)                                                                             cAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                                                                       <  7:94647/149‑1 (MQ=255)                                                                                               gCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                                                                     >  8:67460/1‑133 (MQ=255)                                                                                               gCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                                                                     <  7:67460/133‑1 (MQ=255)                                                                                                cTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTGTTCGCAAGCtt                                                    >  3:7434/1‑149 (MQ=255)                                                                                                    cgcgTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGa                                                <  3:104630/149‑1 (MQ=255)                                                                                                       gTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGAGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGtt                                             >  2:186413/1‑149 (MQ=255)                                                                                                          aTCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGc                                           <  7:107908/148‑1 (MQ=255)                                                                                                           tCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTc                                                            >  6:44722/1‑130 (MQ=255)                                                                                                           tCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTc                                                            <  5:44722/130‑1 (MQ=255)                                                                                                            cAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                                                                               <  1:122902/94‑1 (MQ=255)                                                                                                            cAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                                                                               >  2:122902/1‑94 (MQ=255)                                                                                                              aaGCATCGATAATAAATCCGGGTAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCaa                                      >  3:69742/1‑149 (MQ=255)                                                                                                              aaGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCaa                                      >  4:117979/1‑149 (MQ=255)                                                                                                                  aTCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCt                                  <  8:61764/149‑1 (MQ=255)                                                                                                                   tCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGACATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTATTTCGCAAGCTTCCGAGTTGCATCAACgaa                                  >  4:57151/1‑146 (MQ=255)                                                                                                                      ataataAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCAt                                                                                                                  >  3:159282/1‑65 (MQ=255)                                                                                                                      ataataAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCAt                                                                                                                  <  4:159282/65‑1 (MQ=255)                                                                                                                          taaATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCATTGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaa                          <  3:57151/149‑1 (MQ=255)                                                                                                                          taaATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTGTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaa                          <  4:7434/149‑1 (MQ=255)                                                                                                                             aTCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaaga                        >  7:135164/1‑148 (MQ=255)                                                                                                                                  ggcaggctCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCca                  <  3:117979/149‑1 (MQ=255)                                                                                                                                     aggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagca               <  1:186413/149‑1 (MQ=255)                                                                                                                                     aggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagca               <  8:135164/149‑1 (MQ=255)                                                                                                                                       gcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTca                                >  5:84593/1‑130 (MQ=255)                                                                                                                                       gcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTca                                <  6:84593/130‑1 (MQ=255)                                                                                                                                            aGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAAt        <  4:122410/149‑1 (MQ=255)                                                                                                                                             gatgatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                                                                       >  6:176465/1‑85 (MQ=255)                                                                                                                                             gatgatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                                                                       <  5:176465/85‑1 (MQ=255)                                                                                                                                                gatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGc    >  2:89174/1‑149 (MQ=255)                                                                                                                                                  ttAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTa  >  7:128338/1‑149 (MQ=255)                                                                                                                                                  ttAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTa  >  1:51498/1‑149 (MQ=255)                                                                                                                                                   tAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTa  >  7:69514/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTA  >  NC_000913/3462203‑3462496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.