New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3462350 =	51 (1.290)	5 (0.140)	4/250	4.3	10.2%	coding (377/378 nt) coding (7/588 nt)	gspI gspJ	general secretory pathway component, cryptic putative general secretory pathway component, cryptic
	?	NC_000913	3462401 =	41 (1.120)					coding (58/588 nt)	gspJ	putative general secretory pathway component, cryptic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTC  >  NC_000913/3462255‑3462541                                                                                                                                                   |                                                                                                                                             aCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                                                                                                                            <  2:165487/149‑1 (MQ=255)     aCCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCaa                                                                                                                                        >  1:46051/1‑149 (MQ=255)           aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTa                                                                                                                                  <  2:46051/149‑1 (MQ=255)                   aTTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                                                                                                                          >  7:61764/1‑149 (MQ=255)                   aTTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                                                                                                                          >  4:130769/1‑149 (MQ=255)                       cccAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGt                                                                                                                      <  3:130769/149‑1 (MQ=255)                        ccAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATCGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                                                                                                                     >  8:107908/1‑149 (MQ=255)                         cAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                                                                                                                    <  7:94647/149‑1 (MQ=255)                                           gCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                                                                                                                  >  8:67460/1‑133 (MQ=255)                                           gCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTAt                                                                                                                  <  7:67460/133‑1 (MQ=255)                                            cTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTGTTCGCAAGCtt                                                                                                 >  3:7434/1‑149 (MQ=255)                                                cgcgTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGa                                                                                             <  3:104630/149‑1 (MQ=255)                                                   gTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGAGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGtt                                                                                          >  2:186413/1‑149 (MQ=255)                                                      aTCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGc                                                                                        <  7:107908/148‑1 (MQ=255)                                                       tCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTc                                                                                                         >  6:44722/1‑130 (MQ=255)                                                       tCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTc                                                                                                         <  5:44722/130‑1 (MQ=255)                                                        cAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                                                                                                                            >  2:122902/1‑94 (MQ=255)                                                        cAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtc                                                                                                                                            <  1:122902/94‑1 (MQ=255)                                                          aaGCATCGATAATAAATCCGGGTAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCaa                                                                                   >  3:69742/1‑149 (MQ=255)                                                          aaGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCaa                                                                                   >  4:117979/1‑149 (MQ=255)                                                              aTCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCt                                                                               <  8:61764/149‑1 (MQ=255)                                                               tCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGACATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTATTTCGCAAGCTTCCGAGTTGCATCAACgaa                                                                               >  4:57151/1‑146 (MQ=255)                                                                      taaATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCATTGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaa                                                                       <  3:57151/149‑1 (MQ=255)                                                                      taaATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTGTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaa                                                                       <  4:7434/149‑1 (MQ=255)                                                                         aTCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaaga                                                                     >  7:135164/1‑148 (MQ=255)                                                                              ggcaggctCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCca                                                               <  3:117979/149‑1 (MQ=255)                                                                                 aggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagca                                                            <  8:135164/149‑1 (MQ=255)                                                                                 aggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagca                                                            <  1:186413/149‑1 (MQ=255)                                                                                   gcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTca                                                                             <  6:84593/130‑1 (MQ=255)                                                                                   gcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTca                                                                             >  5:84593/1‑130 (MQ=255)                                                                                        aGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAAt                                                     <  4:122410/149‑1 (MQ=255)                                                                                         gatgatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                                                                                                                    >  6:176465/1‑85 (MQ=255)                                                                                         gatgatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                                                                                                                    <  5:176465/85‑1 (MQ=255)                                                                                            gatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGc                                                 >  2:89174/1‑149 (MQ=255)                                                                                              ttAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTa                                               >  7:128338/1‑149 (MQ=255)                                                                                              ttAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTa                                               >  1:51498/1‑149 (MQ=255)                                                                                               tAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTa                                               >  7:69514/1‑148 (MQ=255)                                                                                                                  acaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                                         >  7:133340/1‑119 (MQ=255)                                                                                                                  acaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                                         <  8:133340/119‑1 (MQ=255)                                                                                                                  acaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTc                            <  4:69742/148‑1 (MQ=255)                                                                                                                    aTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCtt                          >  7:94882/1‑148 (MQ=255)                                                                                                                                          cTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGt                                                          >  2:199425/1‑94 (MQ=255)                                                                                                                                          cTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGt                                                          <  1:199425/94‑1 (MQ=255)                                                                                                                                             gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTc  <  1:89174/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                             ACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTC  >  NC_000913/3462255‑3462541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.