breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsERR2455336_R1.good.fq1,488,516318,695,961100.0%214.1 bases240 bases99.6%
errorsERR2455336_R2.good.fq1,488,516278,656,259100.0%187.2 bases240 bases97.2%
total2,977,032597,352,220100.0%200.7 bases240 bases98.4%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0092734,631,469127.07.3100.0%Escherichia coli BW25113, complete genome.
total4,631,469100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000006438
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000380
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.040

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0092730.79615

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input22:47:57 03 Mar 202322:48:51 03 Mar 202354 seconds
Read alignment to reference genome22:48:51 03 Mar 202322:56:51 03 Mar 20238 minutes 0 seconds
Preprocessing alignments for candidate junction identification22:56:51 03 Mar 202322:57:36 03 Mar 202345 seconds
Preliminary analysis of coverage distribution22:57:36 03 Mar 202323:00:32 03 Mar 20232 minutes 56 seconds
Identifying junction candidates23:00:32 03 Mar 202323:00:40 03 Mar 20238 seconds
Re-alignment to junction candidates23:00:40 03 Mar 202323:03:05 03 Mar 20232 minutes 25 seconds
Resolving best read alignments23:03:05 03 Mar 202323:04:08 03 Mar 20231 minute 3 seconds
Creating BAM files23:04:08 03 Mar 202323:06:43 03 Mar 20232 minutes 35 seconds
Tabulating error counts23:06:43 03 Mar 202323:07:37 03 Mar 202354 seconds
Re-calibrating base error rates23:07:37 03 Mar 202323:07:38 03 Mar 20231 second
Examining read alignment evidence23:07:38 03 Mar 202323:18:39 03 Mar 202311 minutes 1 second
Polymorphism statistics23:18:39 03 Mar 202323:18:39 03 Mar 20230 seconds
Output23:18:39 03 Mar 202323:18:52 03 Mar 202313 seconds
Total 30 minutes 55 seconds