| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | AM260479 | 3,399,144 | Δ1,321 bp | [leuS]–h16_A3140 | [leuS], h16_A3140 | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | AM260479 | 3399144–3400455 | 3400464 | 10–1321 | 23 [0] | [0] 23 | [leuS]–h16_A3140 | [leuS],h16_A3140 |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AM260479 | = 3399143 | NA (NA) | 21 (0.850) | 19/198 | 0.3 | NA | noncoding (2/1313 nt) | ISAE1 | insertion sequence ISAE1 |
| ? | AM260479 | 3400465 = | NA (NA) | intergenic (+32/+53) | h16_A3140/dapB | Transposase ISAE1/dihydrodipicolinate reductase | |||||
GGTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAGCCTCTTTTGAATTTCAAGTGGGTGGTATCGTGGCGGGTTTCAGGTAAAGAGCAACAAGAGCGATGCATAGCAAACTGTTTGAAGCAGCCCTTGGCATAAGCGATCCGTGGTTCGTCCGGG > AM260479/3399015‑3399264 | ggTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgat > 2:273417‑M1/1‑129 (MQ=255)ggTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgat > 1:824683‑M1/1‑129 (MQ=255) cTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgc > 1:894898‑M1/1‑92 (MQ=255) ccGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggc > 1:621420‑M1/1‑84 (MQ=255) ccGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcgg < 2:824683‑M1/133‑50 (MQ=255) cATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgcct > 1:1054009‑M1/1‑80 (MQ=255) gggggAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttactt < 1:37179‑M1/134‑60 (MQ=255) ggggAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttc < 2:621420‑M1/134‑61 (MQ=255) ggAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccc < 1:981910‑M1/116‑45 (MQ=255) ggAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAAGGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccc > 2:981910‑M1/1‑72 (MQ=255) tGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcc > 1:184160‑M1/1‑64 (MQ=255) tGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcc < 2:184160‑M1/129‑66 (MQ=255) tGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggc > 2:1045721‑M1/1‑64 (MQ=255) tGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggc < 1:1045721‑M1/114‑51 (MQ=255) gAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacg < 2:1054009‑M1/134‑73 (MQ=255) tGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcc > 2:1041331‑M1/1‑59 (MQ=255) ttGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttg > 1:15769‑M1/1‑38 (MQ=255) cggcgCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggctt < 2:894898‑M1/134‑102 (MQ=255) ggcgCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggcttg < 1:273417‑M1/134‑103 (MQ=255) ccgGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggcttgtcggcgag > 1:180978‑M1/1‑24 (MQ=255) aTGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggcttgtcggcgaggaaacgggcgg > 1:262827‑M1/1‑13 (MQ=255) agaagacgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggccc < 2:584305‑M1/72‑67 (MQ=255) agaagacgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggccc > 1:584305‑M1/1‑6 (MQ=255) | GGTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAGCCTCTTTTGAATTTCAAGTGGGTGGTATCGTGGCGGGTTTCAGGTAAAGAGCAACAAGAGCGATGCATAGCAAACTGTTTGAAGCAGCCCTTGGCATAAGCGATCCGTGGTTCGTCCGGG > AM260479/3399015‑3399264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |