| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | AM260479 | 3,399,144 | Δ1,321 bp | [leuS]–h16_A3140 | [leuS], h16_A3140 | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | AM260479 | 3399144–3400455 | 3400464 | 10–1321 | 23 [0] | [0] 23 | [leuS]–h16_A3140 | [leuS],h16_A3140 |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AM260479 | = 3399143 | NA (NA) | 21 (0.850) | 19/198 | 0.3 | NA | noncoding (2/1313 nt) | ISAE1 | insertion sequence ISAE1 |
| ? | AM260479 | 3400465 = | NA (NA) | intergenic (+32/+53) | h16_A3140/dapB | Transposase ISAE1/dihydrodipicolinate reductase | |||||
AGGCCCGCGGTTATGTGAGCTTCAAAACCATGCGCACCGTGATCTTCCTGATCGCCGGAAAGCTCGACTTCTCCGCGATCAACCCGCATGCCGCATAACCCACTCATTTTTCAAAAGAGCCAAAGAAGACGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGGGCGG > AM260479/3400336‑3400585 | ggtcgggacagcagtcaggacaacgtttgcgcggcaactggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGAt > 2:273417‑M2/130‑133 (MQ=255)ggtcgggacagcagtcaggacaacgtttgcgcggcaactggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGAt > 1:824683‑M2/130‑133 (MQ=255) ctggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTgcgcgc > 1:894898‑M2/93‑134 (MQ=255) ccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcggc > 1:621420‑M2/85‑134 (MQ=255) ccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcgg < 2:824683‑M2/49‑1 (MQ=255) catccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCt > 1:1054009‑M2/81‑134 (MQ=255) gggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACtt < 1:37179‑M2/59‑1 (MQ=255) ggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTc < 2:621420‑M2/60‑1 (MQ=255) ggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGccc < 1:981910‑M2/44‑1 (MQ=255) ggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaaggcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGccc > 2:981910‑M2/73‑116 (MQ=255) tggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcggc > 2:1045721‑M2/65‑114 (MQ=255) tggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcggc < 1:1045721‑M2/50‑1 (MQ=255) tggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGcc > 1:184160‑M2/65‑129 (MQ=255) tggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGcc < 2:184160‑M2/65‑1 (MQ=255) gaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACg < 2:1054009‑M2/72‑1 (MQ=255) tgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTcc > 2:1041331‑M2/60‑134 (MQ=255) ttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTg > 1:15769‑M2/39‑134 (MQ=255) cggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCtt < 2:894898‑M2/101‑1 (MQ=255) ggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTg < 1:273417‑M2/102‑1 (MQ=255) ccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAg > 1:180978‑M2/25‑134 (MQ=255) atgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGggcgg > 1:262827‑M2/14‑134 (MQ=255) agaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGccc < 2:584305‑M2/66‑1 (MQ=255) agaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGccc > 1:584305‑M2/7‑72 (MQ=255) | AGGCCCGCGGTTATGTGAGCTTCAAAACCATGCGCACCGTGATCTTCCTGATCGCCGGAAAGCTCGACTTCTCCGCGATCAACCCGCATGCCGCATAACCCACTCATTTTTCAAAAGAGCCAAAGAAGACGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGGGCGG > AM260479/3400336‑3400585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |